Labshake search
Citations for New England Biolabs :
601 - 650 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
-
bioRxiv - Genomics 2021Quote: Decapping of 5 µg total RNA was performed with 200 units of yDcpS (NEB M0463S) in 10mM Bis-Tris-HCl pH 6.5 ...
-
bioRxiv - Biophysics 2021Quote: ... 15 µg of the purified nicked minicircle was incubated with T4 ligase (New England Biolabs) and ligase buffer containing 25 µg mL-1 BSA ...
-
bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
-
bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 25 µg of pRS193 lentiviral cloning backbone plasmid was digested by EcoRI-HF (NEB #R3101S) and XbaI (NEB #R0145 ...
-
bioRxiv - Microbiology 2023Quote: ... and DNase was removed using the Monarch RNA Cleanup Kit (50 µg; New England Biolabs). LunaScript RT SuperMix Kit (New England Biolabs ...
-
bioRxiv - Biochemistry 2023Quote: ... Total RNA (2 µg) was first annealed with 200 ng random nonamer primers (NEB S1254S) by incubation at 70°C for 5 min ...
-
bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA), 0.2 μM of each primer and 2 μl of template DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 mM EDTA] plus 1 µL 20 µg/µL lambda phage DNA (NEB, cat # N3011S) and was separated on a 6% polyacrylamide / 0.5x TBE / 1.5 mm gel for 2 hr at 110 V in a Novex electrophoresis system ...
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed on 0.5 µg DNase-treated total RNA using Lunascript RT (NEB) in 20µl reactions ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
-
bioRxiv - Plant Biology 2023Quote: ... 100 µg of dual crosslinked enriched chloroplasts were resuspended in 1X DpnII reaction buffer (NEB) and digested with 20 U DpnII at 37°C overnight with gentle shaking ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
-
bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 µg of total RNA was used with D(T)23VN primer (New England Biolabs), 10 mM dNTPs (New England Biolabs) ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 µg of this plasmid along with 50 units of MfeI restriction enzyme (NEB R3589), which cuts at a single site within the PfHO gene just upstream of the homologous sequence cloned into the donor-repair pGDB plasmid ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µg of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 µg of RNA was used to synthesize cDNA by oligo dT (New England Biolabs) and reverse transcriptase (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of the vector backbone (pSP0769) was linearized using PmeI (NEB, Cat. No. R0560S) for 1h and gel purified.
-
bioRxiv - Systems Biology 2024Quote: ... and VPS4B_KO cell lines.200 µg of proteins were digested with LysC (New England Biolabs) by adding 0.4 µg LysC (Promega ...
-
bioRxiv - Synthetic Biology 2024Quote: ... was coated with 25 µL/well of 2 µg/µL streptavidin (New England Biolabs; N7021S) diluted in 1x HBS and incubated overnight at 4 °C ...
-
bioRxiv - Biophysics 2024Quote: ... was then 5’-end labeled with 2 pmol of ATP [Ψ32P] (12 uCi/uL, 5000 Ci/mmol, Hartmann Analytic GmbH) using 10 units of T4 polynucleotide kinase (NEB, 10 U/µL) in T4 polynucleotide kinase buffer (NEB ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and +1 sites of the promoters controlling transcription of both RNAs were used to amplify the standard pUC19 plasmid (NEB). After PCR amplification samples were digested with DpnI (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples with RNA Integrity Number > 8 were used for library preparation following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Library construction and sequences was carried out at the Biomedical Research Centre Sequencing Facility (University of British Columbia ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA library preparation was performed according to the standard protocol of the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs). The synthesized double-stranded cDNA was end-repaired using NEBNext End Prep Enzyme Mix before ligation with NEBNext Adaptor for Illumina ...
-
bioRxiv - Cell Biology 2022Quote: ... 250ng of RNA from each sample was used to generate cDNA using a standard protocol for ProtoscriptII Reverse Transcriptase (NEB), except with 1uL of Oligo(dT)12-18 (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Riboprobe templates were synthesized from cDNA via standard PCR using opsin specific primers (Table S1) and Q5 High Fidelity DNA polymerase (New England Biolabs). Primers were designed to bind to the coding sequence of target opsins (SWS2B ...
-
bioRxiv - Cancer Biology 2021Quote: ... Qualifying samples (RNA Integrity Number ≥ 9) were then prepped following the standard protocol for the NEB next Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads ...
-
bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction was performed using a 50 µL master mix consisting of 1x standard Taq buffer (New England Biolabs), LCO1490 primer (0.2 mM) ...
-
bioRxiv - Biochemistry 2020Quote: ... λex = 488 nm and λem = 526 nm were used for detection of covalently bound flavin and λex = 670 nm and λem = 633 nm for visualisation of the Blue Prestained Protein Standard (New England Biolabs).
-
bioRxiv - Synthetic Biology 2021Quote: ... Divergent 5’ phosphorylated primers carrying the mutations were used to amplify the whole plasmid and introducer the mutations using the standard Phusion DNA polymerase protocol from NEB. Mutagenesis primers are listed in Supplementary Table 5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... All cDNA libraries were constructed according to the standard protocol of the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs). The synthesized double-stranded cDNA was end-repaired using NEBNext End Prep Enzyme Mix before ligation with NEBNext Adaptor for Illumina ...
-
Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... The vectors for the ΔS2 and ΔS2S3 mutants were obtained via standard site-directed mutagenesis using the Q5 system (New England Biolabs), according to manufacturer specifications ...
-
bioRxiv - Genomics 2022Quote: ... The TdT reation was prepared using 4 μL of the resulting elutant and 5 μL of ice-cold TdT mix (standard Quartz-seq2 condition: 1× Thermopol buffer [New England Biolabs] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Each pool was then converted using an enzyme-based conversion to increase the recovery of single cell gDNA compared to standard bisulfite conversion (NEBNext Enzymatic Methyl-seq, New England Biolabs)102 ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA copies were quantified in 10% of the obtained eluate volume with a one-step RT-qPCR reaction using a standard curve and the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) and previously published TaqMan primers and probe (Corman et al. ...
-
bioRxiv - Microbiology 2020Quote: ... the V1-V3 region of 16S rRNA genes was amplified from 2 ng of DNA in 50 μl reactions containing the following components: 1x Standard Taq Reaction Buffer (NEB), 3 mM MgCl2 (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... at λex = 488 nm and λem = 526 nm for detection of covalently bound flavin and at λex = 670 nm and λem = 633 nm for visualization of the Blue Prestained Protein Standard (NEB).
-
bioRxiv - Microbiology 2020Quote: ... library prep was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). For insertion junction sequencing ...
-
bioRxiv - Immunology 2022Quote: All CER constructs were generated by standard PCR cloning techniques and the PCR products were assembled using the NEBuilder HiFi DNA Assembly (New England Biolabs). CER21 was generated by linking the extracellular and transmembrane domain of human TIM-4 (GenBank™ accession number AAH08988.1 ...
-
bioRxiv - Synthetic Biology 2020Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Vent DNA Polymerase (New England Biolabs (NEB)) ...
-
bioRxiv - Synthetic Biology 2020Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Vent DNA Polymerase (New England Biolabs (NEB)) ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... proteolytic subunits were removed from the expression constructs using blunt end deletion following a standard Q5 mutagenesis workflow (New England Biolabs). Additionally ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from cells grown to OD600 0.4–0.6 in 6 mL of SD[MSG] -ura -his using standard hot-phenol procedure (Köhrer and Domdey 1991) and RNA samples were treated with DNase I (New England BioLabs, USA) according to manufacturer’s protocol ...