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Citations for New England Biolabs :
751 - 800 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA (1 µg) was digested with the methylation-sensitive restriction enzyme HpaII (New England Biolabs) and analyzed by Southern hybridization with a specific biotin-labeled DNA probe (300 ng ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 µg of the lysate was digested with 1000 U micrococcal nuclease (NEB, Cat. # M0247S) for 30 min at 22°C ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using 2 µg of total RNA and M-MuLV Reverse Transcriptase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µg of HMW DNA were de-phosphorylated using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30 min at 37°C followed by 5 min at 65°C ...
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bioRxiv - Genomics 2023Quote: ... 20 µg of total RNA was then treated with 4 units of DNAse I (NEB, #M0303S) for 1 h at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 4 µg of each sample was reverse transcribed using dT priming with Protoscript II (NEB, M0368L) and subsequently treated with 0.5 µL each of Rnase H and Rnase A (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Systems Biology 2024Quote: ... we digested 10 µg of each vector with AvrII (#ER1561; Thermo Fischer) and XcmI (#R0533; NEB) and performed a gel-purification ...
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bioRxiv - Neuroscience 2024Quote: ... using the manufacturer’s protocol except that a Monarch® RNA Cleanup Kit (10 µg; NEB# T2030) was used to purify the rRNA-depleted RNA instead of magnetic beads ...
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bioRxiv - Microbiology 2024Quote: ... Twenty µg of protein was treated with PNGase F as per manufacturer instructions (New England Biolabs). Water was used as the mock treatment ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA in the reaction was purified using the Monarch RNA Cleanup Kit (50 µg, NEB).
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bioRxiv - Microbiology 2024Quote: ... 1 µg of total RNA was processed using NEBNext® rRNA Depletion Kit (Bacteria) (NEB, E7850). Sequencing libraries for RNA-Seq were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genomics 2024Quote: ... 50 nl of RNase I (0.2 U/µl lysate; 500 U/µg RNA, New England Biolabs) was added to each well ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of genomic DNA was incubated with 30 U of T4 DNA Polymerase (NEB # M0203L), 25 µM dATP/dGTP (NEB # N0440S/N0442S respectively ...
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bioRxiv - Molecular Biology 2024Quote: 10 µg of DNase-treated total RNA was digested with 5 U of RNase H (NEB) and 2 picomoles of a chimeric oligonucleotide targeting the MSRs for 20 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2021Quote: ... The RNAs were co-transcriptionally capped with m7G anti-reverse cap analog or ApppG Cap Analog (New England Biolabs, 1411 and Cat#1406). The RNAs were purified using a Purelink RNA Mini Kit (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA extraction was performed using TRI reagent (Cosmo Bio Co., Ltd., Tokyo, Japan) and the Monarch Total RNA Miniprep Kit (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and then the two PCR products were co-transformed into yeast along with pCB05 digested with BstXI (New England Biolabs [NEB], Catalog # R0113S) and XhoI (NEB ...
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bioRxiv - Genomics 2023Quote: ... The resulting cDNA was sent to Biotools Co., Ltd (New Taipei City, Taiwan) for library preparation using the NEBNext® DNA Library Prep Kit (NEB, USA,20015828,20015829), and sequenced for 150bp paired-ends on an Illumina Hiseq 2500 sequencer ...
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bioRxiv - Biochemistry 2021Quote: ... 1μL m6A antibody per sample was coupled to pre-washed Protein G Magnetic Beads (NEB) in 1x reaction buffer (150mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... G or C) and assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The randomized N12-oligonucleotides were commercially synthesized and purified on a reversed-phase column (FASMAC ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... IRES mRNAs were capped with the A(5’)ppp(5’)G cap analog (NEB # S1406L). Post-transcriptional polyadenylation was performed using E ...
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bioRxiv - Genomics 2020Quote: ... The reaction was subjected to end-repair in the same reaction by adding 1 μl of Klenow fragment (New England Biolabs cat. M0210S, 5000 U/ml) and 1 μl of dNTP mix (10 mM dA ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were washed once with PBS for 3 minutes and subjected to nuclear staining with Hoechst (New England BioLabs; 4082S; diluted 1:5000 in PBS) for 5 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were washed once with PBS for 3 minutes and subjected to nuclear staining with Hoechst (New England BioLabs; 4082S; diluted 1:5000 in PBS) for 5 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were synthesized from Integrated DNA Technologies (IDT) and ligated into empty pX459V2.0 vector following standard Golden Gate Assembly protocol using BbsI restriction enzyme (NEB, Cat. No. R3539). The constructed plasmids were purified from 3mL E ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ∼ 1.5 μl of DNA extract was added to 20 μl of PCR reaction mix containing standard PCR buffer and 1 unit of Taq DNA polymerase (Cat # = M0273, New England Biolabs, Beverly, MA). For long-range PCR ...
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bioRxiv - Microbiology 2020Quote: ... were used to clone plasmids using a combination of standard molecular cloning techniques and Gibson Assembly (master mix from New England Biolabs, Ipswich, MA). The plasmid pJS167 (45 ...
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bioRxiv - Microbiology 2022Quote: ... 50 fmol of each amplicon and 15 fmol of YCpBAC vector were assembled using a standard Gibson assembly reaction (New England Biolabs, Ipswich, MA), transformed into E ...
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bioRxiv - Developmental Biology 2021Quote: ... The template for probe synthesis was generated through two rounds of standard PCR method using OneTaq® 2x Master Mix (New England Biolabs, NEB): the first PCRs from cDNA used the gene-specific primers including the T7 linker sequence ...
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bioRxiv - Genetics 2020Quote: ... This exonuclease-treated Klenow amplification was then used as a template in a standard 15-cycle PCR (Taq 2X Master Mix; New England Biolabs, MA. USA) using Illumina IDT-NXT primers carrying dual indexes (Supplemental Data 4).
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Microbiology 2021Quote: ... RNA-Seq libraries were prepared from these enriched samples and from unenriched control “gold standard” samples (i.e. “Unenriched”) using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765) and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Systems Biology 2023Quote: ... The two intermediate libraries were cloned together using high-efficiency cloning and standard restriction enzyme-mediated cloning with enzymes AvrII (New England Biolabs, Ipswitch, MA) and HindIII-HF (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutant libraries were constructed via overlap extension PCR and standard ligation using T4 DNA ligase (New England Biolabs, Beverly, MA). Oligonucleotide primers carrying NNB base triplets were purchased from Integrated DNA Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: 3 µl of cDNA samples or ssDNA standards were added to a 20µl reaction with Luna Universal qPCR Master Mix (NEB, Cat, No. M3003) according to the provided protocol ...
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bioRxiv - Genomics 2024Quote: ... was used for library preparation (Table S1) using standard procedures with the NEB Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). Whole-genome sequencing was outsourced to BGI Japan (Shenzhen ...
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bioRxiv - Immunology 2024Quote: The oligo pool was duplexed by a single cycle of PCR using OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (NEB). A reverse primer (5’-TGGCCCCCGAGGCC-3’ ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the entire plate included 10 µl of OneTaq Quick-Load 2X Master Mix with standard buffer (New England Biolabs, MA, USA), 0.5 µl of each primer (10 µM) ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA templates for the gRNAs were generated by a template-free Phusion polymerase (New England Biolabs) PCR reaction using a common scaffold primer (gRNA scaffold ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Genomics 2021Quote: ... The pellet was dissolved in 200 μl of nuclease free water (New England BioLabs, Massachusetts, USA) over 2 days ...
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bioRxiv - Biochemistry 2020Quote: ... The cell-free extract obtained post centrifugation was applied to an amylose column (New England Biolabs) equilibrated in TNG with 1 mM DTT at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA samples were first treated with DNase I (RNase-free New England Biolabs® Inc.), per the manufacturer’s instructions ...