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Citations for New England Biolabs :
8401 - 8450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Mutagenic PCR constructs were generated by amplifying the upstream and downstream DNA fragments flanking the gene(s) of interest followed by fusion of these fragments with the Janus Cassette using a HiFi assembly master mix (NEB). Transformation of TIGR4 with the mutagenic construct (100ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was amplified in triplicate 25 μl reactions consisting of primers at final concentrations of 0.2 μM and 1X PCR reagent (Q5® Hot Start High-Fidelity 2X Master Mix, New England BioLabs, UK). Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881 ...
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bioRxiv - Microbiology 2023Quote: ... The Q5 Site-Directed Mutagenesis Kit protocol (New England Biolabs) was used for site-directed mutagenesis on previously generated vectors ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (NEB™) was added to a final concentration of 1X per reaction and incubated at 37°C for an additional 15 min ...
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bioRxiv - Microbiology 2023Quote: ... AvcI was then purified using Monarch® RNA Cleanup Kit (NEB™). Purity of the product was evaluated using a 1.0% TBE agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... The AvcI DNA template for in vitro transcription was PCR amplified from pAvcI using Q5 High-Fidelity DNA Polymerase (NEB™). To incorporate the T7 promoter into the final AvcI DNA template ...
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bioRxiv - Microbiology 2023Quote: ... or Q5 polymerase (New England BioLabs).
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bioRxiv - Microbiology 2023Quote: ... or Q5 polymerase (New England BioLabs). Each fragment consisted of an antibiotic resistance cassette (kanamycin or gentamicin resistance genes encoded by aph and aacC1 ...
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bioRxiv - Microbiology 2023Quote: ... the pABA plasmid (from E. coli INV110) was methylated with Dam methylase (New England BioLabs), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The two flanking regions of the chosen genomic exchange region were amplified and joined with PCR fragment carrying antibiotic resistance cassette by overlapping PCR using Q5 polymerase (New England BioLabs). Next ...
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bioRxiv - Microbiology 2023Quote: ... was performed with enzymes and buffers from New England Biolabs® (NEB, Ipswich, MA, USA) according to respective protocols ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of sample was incubated with 1.25 μL of proteinase K (NEB P8107S) for 1 hour at 55°C then 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Microbiology 2023Quote: ... the Phusion DNA polymerase (New England Biolabs) was used ...
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bioRxiv - Microbiology 2023Quote: ... and a 1848 bp fragment (containing the 1131 bp Pfcytb open reading frame) was amplified with primers (SI Appendix, Fig. S2A,B) and Phusion DNA polymerase (NEB). PCR product was verified by gel electrophoresis as single band of predicted size ...
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bioRxiv - Microbiology 2023Quote: ... we used the NEBNext Ultra II End Repair/dATailing module (E7546S, NEB, USA) to prepare 1000 ng DNA from each sample ...
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bioRxiv - Microbiology 2023Quote: HTS was performed on the three RNA extracts after a step of rRNA depletion with the rRNA depletion kit (NEB, Evry, France), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... 1x UDG buffer (NEB), and 1.75 U RNase A at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of RNase inhibitor and 3 μl of T4 RNA ligase 2 (truncated) (New England Biolabs) were added and mix well by pipetting ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μl of T4 RNA ligase (New England Biolabs) and mixed ...
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bioRxiv - Microbiology 2023Quote: ... and polynucleotide kinase (PNK) (New England Biolabs) were sequentially applied on the samples ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using Monarch® Total RNA Miniprep Kits (New England Biolabs) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 μl of Phusion high-fidelity DNA polymerase (New England Biolabs), then reached to final reaction volume of 50 μl by addition of 26.5 μl of Nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared from an equal amount of RNA by reverse transcription using NEB mMuLV reverse transcriptase (New England Biolabs, MA, USA). The list of primers used for amplification is shown in supplementary table 2 (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (NEB; 18 U), and lysozyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... upon restriction with HindIII and XbaI (NEB). The resulting construct was called pybtP:GFP28 ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Microbiology 2023Quote: Mutagenesis vectors were constructed by Gibson Assembly (New England Biolabs) on the pSEVA612S backbone and were maintained in E ...
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bioRxiv - Microbiology 2023Quote: Protein expression vector pMAL-c5Xa (NEB) was used as the backbone for constructing the mCherry reporter ...
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bioRxiv - Microbiology 2023Quote: ... coli cells using the manufacturer’s protocol (NEB, C3019). The cells were plated on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... predigested with NotI-HF (NEB, Cat# R3189L) and BamHI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression of NTCP, inserts encoding NTCP cDNA were cloned into a pCAGGS vector (Niwa et al., 1991) predigested with EcoRI-HF (NEB, Cat# R3101M) and NheI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High-Fidelity DNA Polymerase (New England Biolabs), a forward primer binding to the spliced leader sequence and a reverse binding to the VSG 3’untranslated region ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis with ProtoScript II First Strand cDNA Synthesis (New England Biolabs). The sequences were then amplified ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High-Fidelity DNA Polymerase (New England Biolabs), a forward primer binding to the spliced leader sequence and a reverse binding to the VSG 3’ untranslated region ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of sample was incubated with 1.25 μL of proteinase K (NEB P8107S) for 1 hr at 55°C then 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... The eluted gp120 coree protein was deglycosylated overnight in a 37 °C water bath with Endoglycosidase Hf (New England Biolabs). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... the lentiCRISPRv2 vector was digested with BsmBI (NEB), gel purified using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 0.02% RNase free bovine serum albumin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Immunology 2023Quote: ... and KpnI (NEB, R3142S), using the In-Fusion kit (Takara ...
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bioRxiv - Immunology 2023Quote: Genomic DNA corresponding to selected and diversity control samples were PCR amplified with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) as described in20 ...
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bioRxiv - Immunology 2023Quote: ... Successful ligation was confirmed by Sanger sequencing of DH5a (NEB) colonies transformed with the ligation reaction ...
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bioRxiv - Immunology 2023Quote: ... using Gibson assembly (New England Biolabs). After verification of the correct sequence ...
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bioRxiv - Immunology 2023Quote: ... The multiplex products were used as templates to the second PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs Inc., M0530L) that barcodes each well/perturbation separately ...
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bioRxiv - Immunology 2023Quote: Phage DNA was subjected to two rounds of PCR using Q5 High-Fidelity 2X Mastermix (NEB), as previously described (Garrett et al. ...
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bioRxiv - Microbiology 2023Quote: ... Proteinase K (NEB) and incubated at 56 degrees C overnight ...
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bioRxiv - Immunology 2023Quote: ... Library preparation was done using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, USA) following manufacturer recommendations ...