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Citations for New England Biolabs :
8201 - 8250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and 15 units of Phi29 polymerase (New England Biolabs). We used a PCR machine (SimpliAmp ...
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bioRxiv - Genomics 2023Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB8_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Molecular Biology 2023Quote: ... These immuno-enriched DNA samples could be used for NGS after DNA library construction with NEBNext® UltraTM II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co. ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Microbiology 2023Quote: ... Cloning was performed using restriction enzymes (New England Biolabs & Vivantis Technologies), Quick CIP and T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The template was gel-purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Massachusettes, USA) and transformed into UKMCC1015 electrocompetent cells ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol and cDNA library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). DNA library quality control was done by measuring concentration using the Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Taq DNA polymerases (New England Biolabs) and 2xTaq PCR MasterMix (SolarBio ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB9_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Genomics 2023Quote: ... 1× Phi29 reaction buffer (New England Biolabs), and 15 units of Phi29 polymerase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) (New England Biolabs C2527H) was transformed with pRSET B-GA-MatryoshCaMP6s ...
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bioRxiv - Genomics 2023Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of Phi29 Random Hexamer Primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain was purchased from Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DH5α (NEB). Escherichia coli strains and plasmids used are listed in Supporting Information ...
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bioRxiv - Synthetic Biology 2023Quote: ... and T4 DNA Ligase (NEB), cycling as described above ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (all NEB) at 37°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... we amplified the oligos for each tile (Q5 Hot Start High-Fidelity 2X Master Mix, NEB). In parallel ...
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bioRxiv - Synthetic Biology 2023Quote: ... BbsI-HF (NEB), and T4 DNA Ligase (NEB) ...
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bioRxiv - Genomics 2023Quote: ... Twist BioScience) and then assembled into pNL3.3 enhancer reporter plasmids using Gibson Assembly (New England Biolabs). Each cloned reporter plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Genomics 2023Quote: ... then ligated into a PB-CRISPRia expression plasmid linearized with BstXI and BlpI (New England Biolabs). Chemically competent Stable E ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼40 µg of bulk RNA was subjected to poly(A) selection using Oligo d(T)25 magnetic beads (NEB) followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and then subjected to end healing using T4 PNK (New England BioLabs). Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs) ...
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bioRxiv - Genomics 2023Quote: ... one female and one male HRFI) were artificially methylated using the M.Sss1 Enzyme (New England BioLabs) following the manufacturer instruction ...
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bioRxiv - Genomics 2023Quote: ... treated the digestion with calf intestinal alkaline phosphatase (CIP, New England Biolabs) and purified the digested plasmid using SPRI beads ...
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bioRxiv - Genomics 2023Quote: ... We prepared a miniprep of the first Gibson assembly and validated its restriction profile with EcoRV (New England Biolabs). The miniprep of the first Gibson assembly was digested with NotI (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... and the mutagenesis was confirmed by digesting the amplicon with HinfI (New England Biolabs) (the PEX11 gRNA sequence contains a HinfI restriction site ...
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bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... We digested the plasmid pRS31N with SacII (New England Biolabs), treated the digestion with calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Genomics 2023Quote: ... 0.64 μL 10 U μL-1 T5 exonuclease (New England Biolabs), 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... A LEU2 single integration vector was used for cloning the fragments by Gibson assembly (NEB). In the strains carrying overexpression transgenes ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 ng of the intermediate plasmid products were incubated with 10 uM random hexamer primers and 1X phi29 DNA polymerase buffer (NEB) at 95°C for 3 minutes and then cooled to room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cross-linked DNA was digested using 400 U of EcoRI-HF® (R3101 New England BioLabs® Inc) overnight at 37 °C and the enzyme was inactivated with 1% SDS at 65 °C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... appropriate number of amplification cycles x (98C - 10sec, 63C – 30sec, 72C – 1 min) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs). Library quality was assessed using High Sensitivity DNA Chips with the Agilent 2100 Bioanalyzer System (Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... PolyA mRNA was isolated using the NEBNext® Poly(A) mRNA magnetic isolation module (E7490, New England Biolabs, Ipswich, MA). RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB) for 3 h at 37⁰C ...
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bioRxiv - Neuroscience 2023Quote: ... The region of interest was amplified using Phusion® High-Fidelity PCR Kit (New England Biolabs, Cat. No. E0553S). The PCR product was purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... and amplified using NEBnext High-Fidelity 2x PCR Master Mix (NEB, M0541L) and custom indexed primers68 ...
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bioRxiv - Microbiology 2023Quote: ... DH5α (NEB), DH10ß/TOP10 (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... The TRAK1 construct was made through assembling His-ZZ-TEV-SNAP tag and TRAK1(1-395) into a pACEBac1 vector via HIFI DNA assembly kit (NEB). All constructs were verified using whole plasmid sequencing from Plasmidsaurus.
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bioRxiv - Biophysics 2023Quote: ... Amylose affinity resin (NEB) was used for purification of fusion protein in batch which was eluted in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Neuroscience 2023Quote: Quantitative (q) RT-PCR was carried out using the Luna Universal qPCR Master Mix (NEB) in the CFX384 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The isolated mRNA samples were used to synthesize cDNA using ProtoScript II Reverse Transcriptase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Whole metagenome sequencing libraries were prepared from 26 µL of DNA solution using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs). The DNA was purified and size selected to remove excess adaptors and adaptor dimers using Ampure XP beads (Beckman Coulter Life Sciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... as well as on the website for the original pLentiCRISPRv2 plasmid (Addgene #52961).48,49 Plasmids were transformed into NEB Stable bacteria (New England Biolabs, MA) and colonies were screened for sgRNA inserts by Sanger sequencing of the ligation site ...
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bioRxiv - Microbiology 2023Quote: ... For the restriction digest 1 µg of genomic DNA from UACa20 and als4112Δ was incubated for 1 hour at 37 °C with the restriction endonuclease BamHI-HF (New England Biolabs (NEB), Ipswich ...