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Citations for New England Biolabs :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs, Ipswich, MA, USA) according to the recommendations of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... 1μg of NotI-linearized plasmid was subjected to in vitro transcription using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, Ipswich, MA, USA). For use in ELISpot ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Semi-quantitative PCR was performed using Taq DNA polymerase with standard Taq buffer (New England BioLabs Cat # M0273S). PCR products were analyzed on 2% Agarose gels with 0.5 ng/L Ethidium bromide using a 1kb Plus DNA Ladder (New England BioLabs Cat # N3200S ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The washed pellet was resuspended in TM2 buffer with 1 mM CaCl2 and 12000 U of MNase (New England Biolabs), incubated at 23°C for 15 minutes and the reactions stopped by addition of 0.5 mM EGTA ...
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bioRxiv - Genomics 2023Quote: ... run and qPCR with NEBNext Library Quant kit (New England Biolabs) respectively ...
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bioRxiv - Genomics 2023Quote: ... and 1x CutSmart buffer (NEB) at 37 °C for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... 3 µL 10x NEBuffer 4 (NEB), 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP ...
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bioRxiv - Genomics 2023Quote: ... nick ligation was then performed in a 30 µL reaction with 3 µL of 10x rCutSmart Buffer (NEB), 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+ ...
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bioRxiv - Genomics 2023Quote: ... 9.6 U Bst 2.0 (NEB), 9.6 U Bst 3.0 (NEB) ...
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bioRxiv - Genomics 2023Quote: ... in a 70 µl reaction with 1x CutSmart buffer (NEB) for 20 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 1 mM ATP (NEB), 4 mM DTT (Promega) ...
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bioRxiv - Genomics 2023Quote: ... 9.6 U Bst 3.0 (NEB), or 9 U Isopol SD+ (ArcticZymes) ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Genomics 2023Quote: ... and 7.5 U of Klenow fragment 3’→5’ exo-(NEB), except for input DNA that is degraded (e.g. ...
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bioRxiv - Genomics 2023Quote: Input DNA was fragmented with 0.14 U/µL of Hpy166II restriction enzyme (NEB) in a 70 µl reaction with 1x CutSmart buffer (NEB ...
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bioRxiv - Genomics 2023Quote: ... 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+) (NEB), and 15U of E ...
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bioRxiv - Genomics 2023Quote: ... 170-200 ng of library was indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The manufacturer’s protocol was followed with the following deviations ...
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bioRxiv - Genomics 2023Quote: ... pOPO374 was linearized with primer introduced ends through PCR by Q5 polymerase (NEB). The resulting PCR product was then circularized by treatment with PNK (NEB ...
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bioRxiv - Genomics 2023Quote: ... The resulting PCR product was then circularized by treatment with PNK (NEB) and T4 ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... and T4 ligase (NEB) in an overnight reaction at 16C.
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bioRxiv - Genomics 2023Quote: ... DNA was digested in situ with MboI (NEB) and Csp6I (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA from single adult male flies was extracted using using the Monarch Genomic DNA Purification Kit (New England Biolabs), using a protocol we developed (dx.doi.org/10.17504/protocols.io.bp2l694qklqe/ v1 ...
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bioRxiv - Genetics 2023Quote: ... 2 μL of genomic DNA prep was fragmented by restriction digest in 20 μL reactions with EcoRV-HF and HinDIII-HF (New England Biolabs) for 2h ...
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bioRxiv - Genetics 2023Quote: ... 1 ng of oligoarray library DNA was PCR amplified for 12 cycles in 40 μL reactions using Q5 High-Fidelity DNA Polymerase (NEB) and primers specific to the right or left end library ...
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bioRxiv - Genetics 2023Quote: ... 5 U StuI (NEB), CutSmart buffer (NEB) ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Genetics 2023Quote: ... After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646, NEB, MA, USA), 1nL mixture was micro-injected into single-cell embryos ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Genetics 2023Quote: ... and ligated into pWS1728 (BsrGI and AflII digested) using high concentration T4 DNA ligase (NEB M0202T). Ligations were transformed into DH5⍺ E ...
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bioRxiv - Genetics 2023Quote: The yeast plasmids recovered after thermoselection were subject to a shortened PCR with 15 cycles with oligonucleotide pairs oWS1408 (5°-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAGCATATAATCCCTGCTTTA-3°) and oWS1409 (5°-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCCAGGGGTGGTGCAAACTATG-3°) using a high-fidelity Q5 polymerase (New England Biolabs, Ipswich, MA) to attach overhang sequences ...
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bioRxiv - Developmental Biology 2023Quote: ... per manufacturer ’s instructions (NEB). Library quality was assessed by Fragment Analyzer NGS (Agilent) ...
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bioRxiv - Developmental Biology 2023Quote: ... 50μM dNTPs and 0.1U of Bst3.0 (NEB) or 0.8U of MRT (ThermoScientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... then poly(A) RNA was extracted using the Magnetic mRNA Isolation Kit (NEB). Immunoprecipitation of methylated RNA (MeRIP ...
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bioRxiv - Developmental Biology 2023Quote: ... and then ligated using T4 DNA ligase (NEB, M0202S) to obtain the plasmid pAW63-Tex10 ...
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bioRxiv - Developmental Biology 2023Quote: ... and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were digested with Cac8I (R0579, New England Biolabs), which cuts the wildtype allele ...
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bioRxiv - Developmental Biology 2023Quote: ... transcribed with T7 polymerase (E2040S, New England Biolabs) and purified using mirVana miRNA isolation kit (AM1560 ...
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bioRxiv - Cell Biology 2023Quote: Libraries were prepared on an automated liquid handler (Biomek i7) using the NEBNext Ultra II DNA library preparation kit (NEB, E7645), according to the manufacturer’s instructions and without size selection ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl of 10× CutSmart buffer (NEB), 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei ...
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bioRxiv - Immunology 2023Quote: ... Digested bovine serum albumin (New England Biolabs cat # P8108S) was analysed between each sample to avoid sample carryover and to assure stability of the instrument and QCloud (Chiva et al. ...
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bioRxiv - Cell Biology 2023Quote: ... NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) was used to prepare libraries for further sequencing according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... coli (NEB). Rabbit polyclonal antibodies were raised against the purified polypeptides.
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bioRxiv - Cell Biology 2023Quote: ... 500ng of total RNA were used for RNA-Seq library preparation with the NEBNext Ultra II RNA Library Prep Illumina Kit (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center within the NYUAD Core Technology Platform) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and mutated by PCR to generate the disease variant alleles (New England Biolabs #E0554S). The utilized mutagenic primers are as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... 13.5% PEG8000(NEB) was added to the RT solution ...
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bioRxiv - Developmental Biology 2023Quote: ... after digesting the plasmid with BsmBI (NEB). The sgRNA was transcribed from the BamHI linearized pT7-gRNA vector using the MEGAshortscript T7 kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...