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Citations for New England Biolabs :
8251 - 8300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Genomics 2023Quote: ... Lambda DNA was ordered from New England Biolabs (NEB). For experiments on the human genome ...
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bioRxiv - Genomics 2023Quote: ... 1 uL of QuickCIP (New England Biolabs #M0525S) was added directly to the backbone digestions and incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used to enrich for polyA mRNA ...
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bioRxiv - Genomics 2023Quote: ... CBS 112042+ and CBS 124.78+ were sequenced on R9.4.1 flowcells using the LSK108 kit with 3 μg DNA as input for the end prep reaction (NEB ULTRA-II EP ...
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bioRxiv - Genomics 2023Quote: ... 1.25 uL T4 ligase (New England Biolabs), 3 uL of 10x T4 ligase buffer (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... The two oligos were combined in equimolar amounts and phosphorylated using T4 PNK enzyme (New England Biolabs #M0201S) for 30 minutes at 37°C and then slowly annealed in a thermocycler to make the final double-stranded fragment with sticky ends for cloning into the all-in-one AAV constructs ...
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bioRxiv - Genomics 2023Quote: ... a hybridization reaction was carried out in 1X Buffer 2 (NEB). 10 µM pool of designed oligomers and 10 µM of a complementary-overlap-containing-oligomer were first denatured at 95°C for 15 seconds and allowed to hybridize at 43°C for 5min ...
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bioRxiv - Genomics 2023Quote: ... This involved repairing and 3’ adenylation of 1 µg of HMW genomic DNA with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Modules (New Englang Biolab, NEB). Sequencing adapters were then ligated using the NEBNext Quick Ligation Module (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... adding 1-2ul ApaLI (NEB #R0507L) and 5ul 10X Cutsmart to 43-ul samples and incubating at 37°C for at least 2 hr (or up to overnight).
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bioRxiv - Genetics 2023Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... YCp50-RD was digested with restriction enzymes AgeI-HF (NEB Cat#R3552S) and BstEII-HF (NEB Cat#R3162S) ...
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bioRxiv - Genetics 2023Quote: Total protein extracts were prepared with 2.106 cells re-suspended in 50 μL of loading buffer (B7709S, Biolabs) containing 1% SDS ...
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bioRxiv - Genetics 2023Quote: ... 1ug of RNA was used to synthesize cDNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat #E6560L). 50ng of cDNA was used to perform qPCR with PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
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bioRxiv - Genetics 2023Quote: ... using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7-Rnf212bC9A ...
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bioRxiv - Genetics 2023Quote: ... this mismatch creates a PstI (New England Biolabs, #R3140) restriction enzyme site in the amplified product derived from the wild-type DNA template ...
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bioRxiv - Genetics 2023Quote: pASHH3::3xFLAG-ASHH3-HA was constructed using NEBuilder HiFi DNA assembly Mix (New England Biolabs) and pPLV01 vector41 ...
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bioRxiv - Genetics 2023Quote: ... PCR products (380bp in size) were then digested using AvaI (NEB #R0152L) and separated on agarose gel ...
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bioRxiv - Genetics 2023Quote: ... YCp50-RD was digested with restriction enzymes NotI-HF (NEB Cat#R3189S) and PvuII-HF (NEB Cat#R3151S) ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 5 mM 7-deaza-dGTP (NEB), 1 ul of 25 mM MgCl2 (Qiagen ...
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bioRxiv - Genetics 2023Quote: CAAGCAGAAGACGGCATACGAGAT-3’) and Phusion DNA polymerase (New England Biolabs,
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Inc.) at Novogene and sequenced on an Illumina NovaSeq 6000 (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... Library preparation was done using the NEBNext® UltraTM II DNA Library preparation kit (NEB, #E7103L). Libraries were sequenced on single-end 50 bp (1×50 bp ...
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bioRxiv - Genomics 2023Quote: ... Each reaction contained 3 uL BbsI (New England Biolabs), 1.25 uL T4 ligase (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Genomics 2023Quote: ... The ligation products were treated with Exonuclease V (New England Biolabs) in each 96-well plate ...
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bioRxiv - Genetics 2023Quote: ... PCR on cDNA was performed with Q5 polymerase (NEB, M0491) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... we PCR amplified the DNA using Q5 polymerase (NEB, M0491) and Lambda DNA (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... and SmaI (Catalog no. R0141S, NEB). These sites were subsequently used to introduce the PCR product into linearized pmiRFP670-N1 plasmid (Catalog no.79987 ...
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bioRxiv - Developmental Biology 2023Quote: ... the initial backbone was linearized with XbaI (Catalog no. R0145T, NEB) and BbsI (Catalog no ...
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bioRxiv - Developmental Biology 2023Quote: ... Using MluI (Catalog no. R3198S, NEB) the expression vector was linearized ...
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bioRxiv - Developmental Biology 2023Quote: ... the 3′ ends of small RNA were ligated to an adapter using T4 truncated RNA ligase (Lucigen, Cat# LR2D11310K and NEB, Cat# M0242L), followed by the ligation of the 5′-end adaptors by T4 ligase ...
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bioRxiv - Genomics 2023Quote: Hi-C single-index library preparation of MCF7 cells was performed as previously described using MboI (New England Biolabs) restriction enzyme (30).
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bioRxiv - Genomics 2023Quote: ... all final Illumina compatible ERα STARRseq and ERα-focused STARR-seq capture libraries were prepared by PCR amplification (7 cycles) with NEBNext universal and single indexing primers (NEB), and were sequenced on Illumina NovaSeq 6000 (150bp Paired-End).
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bioRxiv - Genomics 2023Quote: Each 900 ng of high molecular weight NA12878 DNA was preprocessed by first blocking at the 3’ ends with Klenow (exo-) (NEB) in the presence of 10 µM dideoxynucleotides and 1X NEBuffer 3.1 for 30m at 37°C ...
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bioRxiv - Genomics 2023Quote: ... an exonuclease treatment was carried out at 37°C for 1h with 10U of exonuclease I (NEB) in 1X exonuclease buffer (NEB) ...
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bioRxiv - Genomics 2023Quote: ... and SalI-HF (NEB) digested hSTARR-ORI plasmid (Addgene plasmid #99296 ...
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bioRxiv - Genomics 2023Quote: ... was pre-incubated in 1× NEBuffer 3.1 (NEB) with 25 pmol sgRNA at 37°C for 15min to form Cas9-Ribonucleoprotein complex ...
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bioRxiv - Genomics 2023Quote: ... 1000 ng of Lambda Phage (NEB) was added to the tube ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from dissected E14.5 DRG was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was synthesized with iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... using T4 DNA Ligase (Catalog no. M0202S, NEB). The protein coding sequence of H2B-miRFP670 was then amplified via PCR introducing attB sites to eventually clone the PCR product into pDONR221 (Catalog no ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs). The comparative 2-ΔΔCT method was used to determine relative expression of the G9a cKO samples to compared the expression level of controls ...
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bioRxiv - Developmental Biology 2023Quote: ... and BbsI (Catalog no. R0539S, NEB) and a gBlock (IDT ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 5-10 ng of input RNA was used to produce libraries with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) which were sequenced on an Illumina HiSeq 3000 to produce 50 bp single-end reads ...
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bioRxiv - Developmental Biology 2023Quote: ... The DNA was PCR amplified for 12 cycles using indexed primers (New England BioLabs, E7335S or E7500S) to barcode samples ...
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bioRxiv - Developmental Biology 2023Quote: ... Then <5 ng ChIP sample DNA or <20 ng input DNA was used for sequencing libraries using the NEB Next Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645). In Step 1.3 (End Prep) ...
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bioRxiv - Developmental Biology 2023Quote: ... using the Q5 site directed mutagenesis kit (NEB, Ipswich, MA) and primers RA1318 and RA1063 ...
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bioRxiv - Genetics 2023Quote: ... The annealed oligos were then Klenow filled by combining 0.8 μl Klenow polymerase (exonuclease negative, NEB) with 1.35 μl of 1mM dNTPs with 40 μl of product to fill in the barcode oligo ...
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bioRxiv - Genetics 2023Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped with a midiprep kit ...