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Citations for New England Biolabs :
7851 - 7900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S), 1mM ATP ...
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bioRxiv - Biochemistry 2023Quote: IVT and co-transcriptional capping reactions were performed in 30 μL reactions containing 1x T7 RNA polymerase buffer (40 mM Tris-HCl, 20 mM MgCl2, 1 mM DTT, 2 mM spermidine, pH 7.9; New England Biolabs), 5 mM each NTPs ...
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bioRxiv - Biochemistry 2023Quote: ... The gRNA oligos were incorporated into gRNA-Cas9 vector using NEBuilder HiFi DNA Assembly kit (New England Biolabs). Each donor oligo contains a point mutation that replace original codon with alanine or stop codon flanked by 40 nt homologous recombination sequences on both 5’ and 3’ (all donor sequences and gRNA vectors are shown in supplementary files) ...
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bioRxiv - Biochemistry 2023Quote: ... Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL, New England Biolabs, M0201) and 0.42 µM [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 50 units of alkaline phosphatase (calf intestinal, QuickCIP, New England BioLabs) overnight at 37 °C while shaking ...
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bioRxiv - Biochemistry 2023Quote: ... All MeOp mutants were created using site-directed mutagenesis (NEB) from the parent construct ...
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: ... “no SDS” loading dye (New England Biolabs, UK; 4 µL) was added to each sample (15 µL ...
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bioRxiv - Biophysics 2023Quote: ... The probes were then digested with 4 uL RNaseH (New England Biolabs M0297S) and 4 uL RNaseA (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... and KpnI-HF (NEB R3142), after which the largest fragment (11,552 bp ...
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bioRxiv - Biophysics 2023Quote: ... This plasmid was used as a template for G2019S and I2020T site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Catalog # E0445S). These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... and XbaI (NEB) and purified using NEB minprep kit and ligated with a Cy5 oligo following a previous protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Duplex PCR was performed using Q5 Hotstart 2x Master Mix (NEB) with final primer concentrations of 0.5uM ...
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bioRxiv - Cell Biology 2023Quote: ... were transfected and incubated with 0.5 µM 647-SiR SNAP ligand (S9102S, NEB) and 0.5 µg/ml Hoechst 34580 in DMEM (11965-092 ...
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bioRxiv - Bioengineering 2023Quote: After probe ligation cells were counted and added to the ligase buffer (1X T4 DNA Ligase Reaction Buffer (NEB), 0.4 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: ... according to the manufacturer’s protocol and treated with DNAse I (New England Biolabs). Quantitative real time PCR was run on Applied Biosystems QuantStudio 7 Flex (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo) and dephosphorylation was done later using the Shrimp alkaline phosphatase (rSAP, NEB). An overnight ligation was performed by mixing digested vector and annealed gRNA inserts using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... we added 0.2 ml of DdeI (NEB, R0175L) for the 2×601 ...
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bioRxiv - Cancer Biology 2023Quote: ... by first linearizing these backbones with EcoRI and then performing a Gibson assembly with NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L). To generate the plasmid coding for PIKFYVE-N1939K ...
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bioRxiv - Cancer Biology 2023Quote: ... a Poly(A) tailing reaction was performed for 1 hour according to the HiScribe T7 ARCA manual (NEB, E2060S). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... with the restriction enzymes AvrII and HindIII and cloned into the digested plasmid pGJJ191 (described in 24) using T4 ligase (New England Biolabs). AbundancePCA (aPCA ...
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bioRxiv - Cancer Biology 2023Quote: ... These products were then purified by separation on a 1.0% agarose gel at 100 volts constant for 1 hour and were then purified via the Monarch DNA gel extraction kit (NEB, #T1020L). Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... and the DNA of the SL5-6 domains was then digested using BsaI (New England Biolabs #R0535) followed by Mung Bean Nuclease (New England Biolabs #M0250) ...
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bioRxiv - Biophysics 2023Quote: ... The residual linker was deadenylated using a 5ʹ deadenylase (New England Biolabs #M0331, 50 units per sample) in NEBuffer 1 (New England Biolabs #B7030 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then transformed into 100μL NEB Stables (NEB Cat. #C3040H), followed by maxiprep plasmid isolation.
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Cell Biology 2023Quote: ... libraries were constructed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and NeBNext Multiplex Adapters (NEB ...
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bioRxiv - Immunology 2023Quote: ... were used along with 10% BSA (NEB #B9200) as a blocking agent ...
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bioRxiv - Neuroscience 2023Quote: ... The bead bound α-βγδε fragments were digested with BsaI-HF (NEB) to prepare the 3’ end of the DNA fragments for the subsequent ligation step.
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared by overnight digestion with NlaIII (New England Biolabs (NEB)) and ligation for 4.5 hours with 100 units T4 DNA ligase (Promega ...
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bioRxiv - Biochemistry 2023Quote: Cell pellet from panning experiment containing ∼106 cells was resuspended with 50 μL 0.1 mg/mL RNase A in 1x Phusion® HF reaction buffer (NEB, Cat. B0518S). Cells were vortexed at maximum speed for 1 min ...
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bioRxiv - Biochemistry 2023Quote: ... 5% (v/v) DMSO and 0.5 U Phusion High-Fidelity DNA polymerase (NEB, M0530S). Cycling was performed using the following thermocycler settings ...
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bioRxiv - Microbiology 2023Quote: ... The mRNA was converted to cDNA using the Invitrogen PhotoScript II First-Strand Synthesis System (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 10µM of each primer and 10 μl of OneTaq 2X Master Mix with Standard Buffer (New England Biolabs) was used for double-strand DNA synthesis ...
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bioRxiv - Biochemistry 2023Quote: ... The eluant was then flowed on an amylose resin (NEB), washed with NiA buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... 72°C for 1 min) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Molecular Biology 2023Quote: The resulting linear DNA fragments were assembled using the Gibson Assembly master mix (New England Biolabs). 5 μL of mix was added to 15-20 fmol of linearised vector and 4× excess of insert and topped up to 10 μL with double-distilled water (ddH2O) ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 5 μL of a sense oligo and then added 40 μL of phosphorylation reaction mix containing 1 μL of T4 Polynucleotide Kinase (PNK; NEB, cat. no. M0201S), 5 μL of T4 PNK buffer (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA concentration of each sample was measured with Nanodrop (IMPLEN, Nordic Biolabs) and Agilent TapeStation QC (Agilent Technologies Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The purified library insert was ligated into the digested pBAD33-eCPX backbone using T4 DNA ligase (NEB) for 1 hour at 25℃ ...
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bioRxiv - Cancer Biology 2023Quote: ... via a 20 cycle 50 μL PCR reaction with Q5 high-fidelity DNA polymerase (NEB), using Lib Prep 2 Forward (5’-CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTG-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... followed by PNGase A (NEB; pH 6). The released N-glycans were purified using initially a cation exchange material (Dowex AG50 H+ form ...
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bioRxiv - Bioengineering 2023Quote: ... 10 U of T7 endonuclease I (NEB) was added and incubated for 15 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Cassettes were confirmed by PCR using Q5® High-Fidelity DNA polymerase (NEB) with primers from IDT (Leuven ...
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bioRxiv - Bioengineering 2023Quote: ... 1.0 µL T4 Ligase Buffer (NEB), and 0.5 µL BsaI (10,000 U/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... The expression of each engineered component was validated pre- and post-sort by staining Jurkat cells with an anti-Myc antibody (#2233S, NEB, MA, USA), followed by performing flow cytometry on a FACSCanto (BD Biosciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μl anti-MBP Monoclonal Antibody (New England Biolabs, #E8032L) and 6 μl anti-E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 µg of purified DNA (PCR product after 2nd round PCR after selections) was incubated with PstI-HF (New England Biolabs) at 37 °C for 1 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells and streaked onto Luria-Burtani (LB) plates containing carbenicillin. All-by-all repressor constructs (Fig. 5c) were cloned by digestion with BsiWI-HF (NEB) and BbsI (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...