Labshake search
Citations for New England Biolabs :
7951 - 8000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... dNTPs (NEB N0447L)
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Biochemistry 2023Quote: ... the point mutations to synthesize AID-C12*-dCas9-BE4max and AID-C12*-n’Cas9-BE4max were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat #E0554S).
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bioRxiv - Biochemistry 2023Quote: ... or Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB, Cat # M0531S). PCR products were purified via QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... or Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB, Cat # M0531S). PCR products were purified via QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Indexed DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, Cat # E7645S) with the following specifications ...
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bioRxiv - Plant Biology 2023Quote: ... and then ligated by T4 DNA ligase (NEB). After reversing cross-links ...
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Genetics 2023Quote: ... which were subsequently inserted into the plasmid backbone using NEBuilder (NEB, E2621). The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... and G118V were generated from EGFP-PFN1 plasmid with site-directed mutagenesis (Q5 New England Biolabs) using the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were incubated in NGS (NGS; New England BioLabs, Hitchin, Hertfordshire, UK) diluted in 0.1 M PBS (pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: Small RNA libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs), and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... coli (New England Biolabs) (NiCo21 ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). For both types of libraries ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: Purified CBC or CBC-ARS2147-871 and their mixtures with different His-MBP-tagged partners were loaded onto Amylose resin columns (NEB). Columns were then extensively washed with a buffer containing 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... The pCR8[mZC3H18] plasmid was used as a template to generate subsequent ZC3H18 cDNA constructs that were cloned into a piggyBAC (pB) vector containing an N-terminal MYC tag and BSD selection marker using NEBbuilder HiFI DNA assembly (NEB). OsTIR1-HA ...
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bioRxiv - Microbiology 2023Quote: ... and RNase H in NEBNext® Second Strand Synthesis Reaction Buffer (NEB) at 16 ºC for three hours ...
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bioRxiv - Biochemistry 2023Quote: ... coli inorganic PPase (New England Biolabs, Inc). All reagents were purchased from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μg/ml BSA (New England Biolabs, Inc.), 0.005% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... Each pool was treated with Exonuclease I (NEB) for 30’ at 37°C ...
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bioRxiv - Immunology 2023Quote: ... in vitro transcription was performed over-night with the T7 High Yield RNA polymerase IVT kit (NEB) for 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... RNA was then ligated to the MARSseq ligation adaptor with T4 RNA Ligase I (NEB) at 22°C for 2h ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Molecular Biology 2023Quote: ... Linearized cDNA was PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S).
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bioRxiv - Cell Biology 2023Quote: ... E117G and G118V were generated from GFP-PFN1 plasmid using site-directed mutagenesis (Q5 New England Biolabs) with the following primers ...
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bioRxiv - Microbiology 2023Quote: ... Subtraction of both bacterial and mammalian rRNA was done using the NEBNext rRNA Depletion Kits (NEB), and sequenced on a Novaseq 6000 instrument (Novogene) ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Molecular Biology 2023Quote: All plasmids were constructed with NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and T4 DNA ligase were from NEB Canada (Whitby ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and amplified using NEBNext® High-Fidelity 2x PCR Master Mix (NEB, Cat. #M0541s) for 9 cycles ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB, Cat.#E7103S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Three cDNA samples derived from three independent RNA extractions were synthesized and amplified using Luna®Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the entire region of pET15b-His-sdeA except for the region encoding 1-199 aa of SdeA was amplified with primers 2649/2712 and then the fragment was self-ligated with a Gibson assembly kit (New England Biolabs). For construction of pmGFP-sdeAΔDUB ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... Quick CIP and T4 DNA ligase (New England Biolabs) described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... and m7Gppp5′N cap was added using Vaccinia Capping System (NEB). To induce superovulation ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Poly(A) polymerase (NEB), and m7Gppp5′N cap was added using Vaccinia Capping System (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... and DNase I (NEB) were added to lysate and was then incubated at room temperature on a Nutator mixer ...
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bioRxiv - Bioengineering 2023Quote: ... RNase I (NEB) and DNase I (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Taq DNA polymerase (New England Biolabs) was used for error-prone PCR (EP-PCR) ...
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bioRxiv - Microbiology 2023Quote: ... and HindIII (New England Biolabs, Ipswich, MT, USA) at 37°C for one hour ...