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Citations for New England Biolabs :
8101 - 8150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... and polyA enrichment module (NEB E7490S). Up to 1µg of total RNA was used to make each library ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amplification was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L). The PCR reaction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... dGTP and dTTP by DNA Polymerase I Klenow Fragment (NEB, #M0210L). The resulting blunt-end fragments were religated by T4 DNA ligase (Enzymatics ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), targeting the insert size of 250 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was treated with T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Developmental Biology 2023Quote: ... the pre- microRNA sequence of mir-51 was amplified from genomic DNA using PCR and primers 5’-cggcatcgacgacgacgacggtccgaaaagtccgtctacc-3’ and 3’- cagttggaattctacgaatgaactgtattgctgctgggc-5’ and the vector containing the sequence of rgef-1p and unc-54 3’UTR amplified from plasmid rgef-1p::aak-2::unc-54 3’UTR using PCR and primers 5’-cattcgtagaattccaactgagc-3’ and 3’-cgtcgtcgtcgtcgatgc-5’ were used to generate rgef-1p::mir-51::unc-54 3’UTR by using Gibson assembly (NEB E2611).
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and Magnetic Separation Rack (NEB S1507S) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and T4 DNA ligase (NEB M0202S), then verified through sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... The fragments were cloned into a pCS2+ plasmid linearized with Xho1 and Xba1 restriction enzymes using a Quick Ligation Kit (NEB). Each plasmid was then linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription (using the mMESSAGE mMACHINE sp6 Ultra kit AM1340 ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Prepared CUT&RUN libraries of PRC1ctrl and PRC1cKO Aundiff were sequenced on the NovaSeq 6000 system (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... the mRNA was separated from the isolated RNA using the Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) and Magnetic Separation Rack (NEB S1507S ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... by T4 PNK (polynucleotide kinase, NEB, M0201), purified through a MicroSpinTM G-25 spin column (Cytiva AmershamTM ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were ligated to pUCm-T vector (B522211, Sangon) with Blunt/TA Ligase Master Mix (M0367S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Sequencing libraries were validated using the Agilent Tapestation 4200 (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Both pDN0605 and pKB11 were digested with I-SceI (New England Biolabs, R0694S) and I-CeuI (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and I-SceI (New England Biolabs, R0694S) digested pDN0509 backbone ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligation reactions were performed using T4 DNA ligase (New England Biolabs, M0202S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and subjected to in vitro DNA assembly (Gibson et al. 2009) with SpeI-HF (New England Biolabs, R3133S) digested pDN0603 as backbone ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This was achieved by inserting a synthesized dsDNA fragment dsOPE001 to the SpeI (New England Biolabs, R3133S) and I-SceI (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and PCR-amplified 2.5 mM DNTPs (NEB, CAT#N0447S), 10X PCR buffer (Klentaq ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The backbones of pKB11 and pKB12 were treated with calf intestinal alkaline phosphatase (CIP) (New England Biolabs, M0525S) to prevent self ligation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The digested product of pKB11 was treated with CIP (New England Biolabs, M0525S) to prevent self ligation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This was achieved by first digesting pDN0520 with I-SceI (New England Biolabs, R0694S), I-CeuI (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mEGFP insert was column purified after DpnI (New England Biolabs, R0176S) treatment ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs E6440S) and polyA enrichment module (NEB E7490S) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The Homology-Directed Repair (HDR) template used for gene replacement was assembled into plasmid pBluscript KS-using HiFi assembly (NEB, E2621S). Two independent mutants were obtained based on two different protoplast pools and different sgRNAs (Supplemental Table 1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNAseq libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs E7760S) with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Developmental Biology 2023Quote: ... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 mM dNTPs (NEB, N0447L), 5 mM RNAseOUT (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Then, 2.5 μL of MNase master mix (MNase buffer, 0.125 U/μL MNase (NEB, M0247S), 2 mM DTT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cross-linked DNA was digested with 200 U MboI (NEB, #R0147M), and the digested fragment ends were filled with biotin-14-dATP (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the DNAs of one Jomon dog (MD1) and three late 8th century dogs (SWD_2, SWD_5, and SWD_7) were treated by USER enzyme (NEB) for uracil removal ...
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bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product was subjected to T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Cell Biology 2023Quote: ... Top and bottom oligos were annealed and then phosphorylated by T4 PNK (NEB, Ipswich, MA). Guides were then cloned into PX459 that was cut using BbsI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Guides were then cloned into PX459 that was cut using BbsI (NEB, Ipswich, MA) and ligated using T4 ligase (NEB Ipswich ...
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bioRxiv - Cell Biology 2023Quote: ... using the Gibson Assembly Cloning Kit as instructed by the manufacturer (New England BioLabs). The resulting vector was termed pCEP4-MFAP4 coding for the amino acid sequence M1KAL…IRRA255 ...
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bioRxiv - Cell Biology 2023Quote: ... The template plasmid was digested with DpnI (NEB), and the PCR amplicon was purified using the DNA Clean & Concentrator-5 (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid was amplified using Q5 DNA polymerase (NEB). The template plasmid was digested with DpnI (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... libraries were prepared using NEBNext Ultra kit (New England Biolabs #E7490, #E7770, #E7335, #E7500) and sequenced using a Nextseq500/550 sequencer ...
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bioRxiv - Cell Biology 2023Quote: mNeonGreen-CFTR-WT and mNeonGreen-CFTR-F508del were cloned into pMCB497-pTRE-polyA(-)-pPGK-blastR vector backbone (Leto et al., 2019) using the NEB HiFi Assembly mix (NEB, Ipswitch, MA). CFTR was amplified from pShu-eGFP-CFTR-WT and pShu-eGFP-CFTR-F508del (gift from Jon Hanrahan ...
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bioRxiv - Cell Biology 2023Quote: ... coli cells (New England Biolabs, C2523). Nanobodies were expressed in E ...