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Citations for New England Biolabs :
7801 - 7850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 1 kb plus DNA ladder (NEB)
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bioRxiv - Biochemistry 2023Quote: ... The cDNA was then amplified with Phusion High-Fidelity DNA Polymerase (New England Biolabs, catalog #M0530S) using the following steps ...
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bioRxiv - Neuroscience 2023Quote: ... and the resin was then incubated with TEV protease (New England Biolabs, Cat# P8112S) overnight at 4° C ...
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bioRxiv - Microbiology 2023Quote: ... A second DNA adapter (containing a random-mer of 10 (N10) random bases at the 5′ end) was then ligated to the cDNA fragment 3′ end (T4 RNA Ligase, NEB) in the presence of a high concentration of PEG8000 and dimethyl sulfoxide (DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... pJMP6957 was digested with XhoI (New England Biolabs Cat. No. R0146) and purified (QIAquick PCR Purification Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 20 mg/ml RNase A (NEB) was added ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were generated for this study using Gibson Assembly following the manufacturer’s protocol (E2611; NEB). All pBP73-G vectors were cut with XbaI and XhoI ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... by Gibson assembly (NEB). The resulting vector was pIM179 ...
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bioRxiv - Developmental Biology 2023Quote: ... mixed in a ratio 1:3 (insert:backbone plasmid) and ligated with T4 ligase according to manufacturer’s instructions (Quick Ligation kit, NEB M2200). The ligation reaction was transformed into competent TOP-10 bacteria and plated on agarose plates with Ampicillin ...
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bioRxiv - Bioengineering 2023Quote: ... and NEBuilder® HiFi DNA Assembly Master Mix were purchased from New England Biolabs (NEB, Ipswich, MA, USA). Oligonucleotides for genetic manipulations were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmids were ligated based on Gibson assembly (New England BioLabs master mix) of plasmid backbone and insert sequences ...
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bioRxiv - Bioengineering 2023Quote: ... and 1.25 μl of 10 μM each primer using Phusion high-fidelity DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Plant Biology 2023Quote: ... PolyA mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Afterward ...
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bioRxiv - Plant Biology 2023Quote: ... coli Shuffle cells (NEB). Cultures were grown in LB medium at a temperature of 37 °C to an OD600 of 1.0 – 1.2 ...
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bioRxiv - Plant Biology 2023Quote: ... PCR amplicons were ligated with AgeI- and XhoI- (New England BioLabs) linearized pEAQ-HT vector27 using HiFi DNA assembly mix (New England BioLabs) ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR steps were carried out using Q5 DNA polymerase (NEB).
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bioRxiv - Neuroscience 2023Quote: ... and murine RNase inhibitor (NEB, M0314) at 37 °C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... the RNAs were phosphorylated with T4 PNK (NEB, M0201) supplemented with murine RNase inhibitor at 37 °C for 20 min and purified using MyONETM Silane beads.
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bioRxiv - Neuroscience 2023Quote: ... the 3′ RNA adapter was ligated to the purified RNAs using T4 RNA ligase 1 (NEB, M0437) at 25 °C for 75 min (with gentle agitation every 10 min) ...
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bioRxiv - Plant Biology 2023Quote: ... EM-seq libraries were prepared from sheared DNA using an enzymatic methyl-seq kit following the standard instructions (New England BioLabs), and were subjected to NextSeq550 using 75bp paired-end sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... and plasmid fusion/reassembly were performed with the NEBuilder HiFi DNA Assembly Master Mix (NEB). To create the empty shRNA plasmid (shE) ...
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bioRxiv - Pathology 2023Quote: Total RNA extracted from hearts of Ctrl and Eprs1cKO-homoat 2 weeks post tamoxifen injection were treated with DNase I (NEB) to remove potential genomic DNA in the RNA samples ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA enrichment was done with the NEBNext Poly(A) mRNA magnetic isolation Module (NEB # E7490) (New England Biolabs (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA enrichment was done with the NEBNext Poly(A) mRNA magnetic isolation Module (NEB # E7490) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using an established protocol with NEBNExt Ultra II Directional Library Prep Kit (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences and the Illumina sequencing done by Medgenome (Foster City ...
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bioRxiv - Bioengineering 2023Quote: ... oligonucleotides encoding the crRNA targeting sequences were synthesized (Integrated DNA Technologies) and incubated with T4 polynucleotide kinase (NEB) for 30 min at 37° ...
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bioRxiv - Biochemistry 2023Quote: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT ...
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bioRxiv - Biochemistry 2023Quote: ... crosslinking of Pex1-AzF was performed after a 10 min 30 deg C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S ...
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bioRxiv - Biochemistry 2023Quote: ... coli T7 Express lysY (New England Biolabs, UK) from pET28a/Cas9-Cys (Addgene plasmid # 53261) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified using GeneAid and ∼100 ng of DNA subjected to digestion by NaeI (NEB). Clones that showed no apparent digestion product (due to the modification of the NaeI restriction site ...
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bioRxiv - Biochemistry 2023Quote: ... Inserts were digested with XhoI and HindIII (NEB) for 1 hour at 37 °C and ligated into pcDNA3.1(- ...
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bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were performed using the QuikChange protocol as directed by the manufacturer (Agilent Technologies, New England Biolabs). Upon completion of mutagenesis ...
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bioRxiv - Biochemistry 2023Quote: ... and cloned into BamHI- and XhoI-digested pcDNA5/FRT vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The resultant construct ...
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bioRxiv - Biochemistry 2023Quote: ... a HiFi Assembly kit (New England BioLabs) and a TOPO cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 plasmids targeting sequences BLTP2 open reading frame were prepared by digesting pSpCas9(BB)-2A-Puro plasmid with BbsI (R3539S, NEB) and inserting duplexed primers caccGAGCCAAACACGGTTCCGGA and aaacTCCGGAACCGTGTTTGGCTC or caccGTGCAAGATCTGACGCCGAC and aaacGTCGGCGTCAGATCTTGCAC ...
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bioRxiv - Cell Biology 2023Quote: ... RNA of the best quality from three replicates of each experiment were used for preparation of cDNA libraries for NGS using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis of TBK1 and Rab7 genes was performed using Q5 High-Fidelity 2X Master Mix (NEB). Plasmids are listed in Table 3 and were confirmed by Sanger Sequencing (Keck DNA Sequencing facility ...
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bioRxiv - Developmental Biology 2023Quote: ... Protruding 5’ ends were filled in with Klenow enzyme (NEB) in the presence of [P]-32 dCTP (Hartmann Analytics ...
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bioRxiv - Pathology 2023Quote: ... The two U6-gRNA-trRNA cassettes were amplified and cloned by assembly (NEBuilder, New England Biolabs) in a pAAV already containing a pCAG-eGFP cassette.
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bioRxiv - Biochemistry 2023Quote: ... amylose resin (NEB), or Pierce streptavidin magnetic beads (ThermoScientific) ...
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bioRxiv - Biochemistry 2023Quote: ... and XhoI (NEB) restriction enzymes ...
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bioRxiv - Biochemistry 2023Quote: ... Labelled handles were ligated with the central part with T4 DNA Ligase (New England Biolabs) for 15 h at 16°C followed by 1 h at 37°C before heat inactivation ...
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bioRxiv - Biochemistry 2023Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The whole coding sequence was verified by automated sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... Amylose resin (E8021S, NEB) was added to bind MBP-fused target protein ...
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bioRxiv - Biochemistry 2023Quote: ... then 0.8 units of proteinase K from Tritirachium album (New England Biolabs Inc.) were added ...
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bioRxiv - Biochemistry 2023Quote: ... 500 µL supernatant was applied to 35 µL amylose resin (New England Biolabs) or glutathione resin (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: DNA plasmids were generated using Gibson Assembly (NEB). The unc-54 promoter and unc-54 3’-UTR sequences were amplified from C ...
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bioRxiv - Cell Biology 2023Quote: ... 200 pmol MBP or MBP-Mps11–250 was immobilized on amylose resin (New England BioLabs) equilibrated in lysis buffer ...