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Citations for New England Biolabs :
7751 - 7800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... vector that removed the original scaffold sequence (referred as CRISPRmap-CROPseq) using NEBuilder HiFi DNA Assembly (New England Biolabs E2621). Next ...
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bioRxiv - Genomics 2023Quote: ... resuspended with T4 DNA ligation mastermix (40 µl of T4 DNA Ligase Reaction buffer (NEB), 10% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... Both libraries were ordered as synthesized oligo pools (Integrated DNA Technologies) and PCR-amplified with Q5 DNA polymerase (New England Biolabs M0492) using an optimized two-round amplification strategy to minimize barcode-sgRNA recombination ...
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bioRxiv - Genetics 2023Quote: ... oligonucleotide pools were amplified by 8 to 10 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544X), digested with BstXI and BlpI ...
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bioRxiv - Genetics 2023Quote: ... DNA was amplified with Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs; catalog no. M0494S). For the 1st stage PCR ...
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bioRxiv - Genetics 2023Quote: ... and p.V126V were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA; catalog no. E0552). Primers used for site-directed mutagenesis are given in Appendix 1-table 7 ...
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bioRxiv - Genetics 2023Quote: Twenty nonfunctional 9 base pair barcodes “CellTags” were subcloned into pLentiV_Blast using Q5® Site-Directed Mutagenesis kit (New England Biolabs; catalog no. E0552) (Biddy et al. ...
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bioRxiv - Immunology 2023Quote: ... The pDNA was digested using BsmBI-v2 restriction endonuclease (NEB), followed by purification of the product by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... coli (NEB, C2566) and transformed cells were plated on selective Kan LB agar plates and incubated overnight at 30 °C.
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bioRxiv - Microbiology 2023Quote: ... and strains were obtained from New England Biolabs (NEB, Ipswich, MA) unless otherwise noted ...
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bioRxiv - Microbiology 2023Quote: ... pmacroH2A-3Xflag and pLJM1-BclI were grown in dam-/dcm- competent E.coli (NEB). EGFP-H2A ...
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bioRxiv - Microbiology 2023Quote: ... Six microliters of proteinase K (20 mg/ml) (NEB, P8107S) were added ...
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bioRxiv - Microbiology 2023Quote: ... Six microliters of proteinase K (20 mg/ml) (NEB, P8107S) were added ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA extraction was done using a Monarch® Total RNA Miniprep Kit (New England Biolabs) prior to treatment with TURBO DNAse (Ambion) ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis performed using a LunaScript RT SuperMix kit (New England Biolabs). RT-qPCR was performed on the resulting cDNA using a LightCycler 96 Real-Time PCR system (Roche ...
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bioRxiv - Microbiology 2023Quote: ... U6:sgUPRT (87) by Q5 site-directed mutagenesis (New England Biolabs) to alter the 20-nt sgRNA sequence ...
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bioRxiv - Microbiology 2023Quote: ... followed by the second DNA chain synthesis using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA). The obtained cDNA was used to prepare a NEBNext DNA Library Prep Set for Ion Torrent (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The obtained cDNA was used to prepare a NEBNext DNA Library Prep Set for Ion Torrent (New England Biolabs, Ipswich, MA, USA) and barcoded with the use of Ion Code Barcode Adapters (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... using Quick Ligase kit (New England Biolabs). Ligation was used to transform E ...
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bioRxiv - Microbiology 2023Quote: ... using the Quick Ligase kit (New England Biolabs), and the ligation mix was used to transform the Escherichia coli M15 strain ...
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bioRxiv - Microbiology 2023Quote: ... and digested with SacI and SmaI restriction enzymes (New England Biolabs). This fragment was then ligated in the SacI and SmaI digested pQE80 vector (Qiagen GmbH ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 50 pmol of the in-vitro -transcribed RNA were dephosphorylated using 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal qPCR Master Mix kit (New England Biolabs). Reactions were performed as technical replicates within each of three biological replicates ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 20 pmol of the dephosporylated RNA were 5’-end-labeled (20 µCi of 32P-γATP) in a 20 µL reaction for 1 h at 37°C using 1 U polynucleotide kinase (NEB). Labeled RNA was purified on a G50-column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and assembled in vitro using T4 ligase (M0202M, NEB) along with hairpins at both ends ...
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bioRxiv - Microbiology 2023Quote: ... plasmid backbone using Gibson Assembly (E2621L, NEB). These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210 ...
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bioRxiv - Microbiology 2023Quote: ... the F1 to F7 plasmids were digested with the PaqCI enzyme (R0745L, NEB) and assembled in vitro using T4 ligase (M0202M ...
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bioRxiv - Microbiology 2023Quote: ... and Micrococcal nuclease (2000 U, New England Biolabs) to digest remaining free-floating eukaryotic and bacterial nucleic acids ...
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bioRxiv - Microbiology 2023Quote: The eluted gDNA was resuspended by pipetting to make the beads and elution as homogenous as possible before 20 µL was used in a 40 µL digest using 1 µL of nucleoside digestion mix and 4 µL 10X buffer following the protocol for Nucleoside Digestion Mix (NEB) in an unskirted PCR plate ...
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bioRxiv - Immunology 2023Quote: ... and BspEI (New England Biolabs, R0540) were used to clone the scFv fragment in a vector optimized for mRNA expression (pDA ...
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bioRxiv - Immunology 2023Quote: Plasmids with CAR constructs were linearized by digestion with the restriction enzyme SalI (New England Biolabs). mRNA was produced using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit ( AMB13455 ...
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bioRxiv - Immunology 2023Quote: ... the restriction enzymes BamHI-HF (New England Biolabs, R3136) and BspEI (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... RNA-seq libraries were prepared using a protocol modified from the NEBNext Ultradirectional (Illumina® -NEB Cat# E7760L) library preparation protocol ...
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bioRxiv - Microbiology 2023Quote: ... and the DNA library preparation was performed using Q5 high-fidelity DNA polymerase (New England Biolabs). Peak-calling was performed as previously described [65].
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bioRxiv - Biophysics 2023Quote: DraIII-HF (New England Biolabs (NEB), R3510S ...
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bioRxiv - Biophysics 2023Quote: ... DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: DraIII-HF (New England Biolabs (NEB), R3510S ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Escherichia coli Topoisomerase I (NEB, M0301) was used to relax a supercoiled circular DNA plasmid ...
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bioRxiv - Biophysics 2023Quote: ... RNA was purified using Monarch RNA Clean-up Kit (50 μg) (NEB, T2040S).
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bioRxiv - Molecular Biology 2023Quote: ... a site-directed mutagenesis kit (New England Biolabs Q5) was used ...
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bioRxiv - Biophysics 2023Quote: ... After 4 hours of incubation (required for transcription) RNA was directly treated with 4 units of DNase I (NEB, M0303S) to remove linear or circular DNA used as a template for transcription (see details in Supplementary Methods) ...
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bioRxiv - Biophysics 2023Quote: Purified linear and circular DNA were both in vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S). 240 ng reaction was prepared according to the manufacturer’s recommendation (see details in Supplementary Methods) ...
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bioRxiv - Biochemistry 2023Quote: ... and Exo I exonuclease (NEB, USA) with the DNAs listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Genetics 2023Quote: ... which were subsequently inserted into the plasmid backbone using NEBuilder (NEB, E2621). The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).