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Citations for New England Biolabs :
7901 - 7950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The ligations were transformed into high efficiency (1-3 × 109 CFU/μg pUC19 DNA) competent cells (NEB C3040). The transformations were serially diluted and plated on LB with ampicillin (100 μg/ml) ...
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bioRxiv - Synthetic Biology 2023Quote: The in vivo and in vitro synthesized DNA were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance ...
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bioRxiv - Synthetic Biology 2023Quote: ... small insertions or mutations were inserted into plasmids using Q5 site-directed mutagenesis (NEB CN# E0554S) according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019) ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.
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bioRxiv - Systems Biology 2023Quote: ... T4 ligation (NEB) was performed for 20 minutes at 37°C and the 5-10 μL ligation reaction was transformed into Stbl3 E ...
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bioRxiv - Synthetic Biology 2023Quote: ... Isothermal DNA assembly [33] using the HiFi DNA Assembly Master Mix was used according to manufacturer’s protocols (NEBuilder, New England Biolabs, Ipswich, MA) to assemble pathway gene overexpression plasmid constructs ...
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bioRxiv - Systems Biology 2023Quote: sgRNAs were transcribed in vitro using the EnGen sgRNA Synthesis kit (NEB) with oligonucleotides containing the protospacers ...
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bioRxiv - Cancer Biology 2023Quote: ... and Calf Intestine Phosphatase (NEB) was added to 10 U/mL ...
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bioRxiv - Systems Biology 2023Quote: ... and 5 μL of 400 U/μl T4 DNA ligase (NEB). The ligation reaction was incubated for 4 h at 16°C with occasional inversion of the tube (every 1 h) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (New England Biolabs) were transformed with either of the five plasmids encoding CFP-E3-Switch ...
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bioRxiv - Synthetic Biology 2023Quote: DNA ladder: 1 kb DNA Ladder (NEB) for gel electrophoresis
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 500 ng of genomic DNA was fragmented using NEBNext Ultra II FS DNA Fragmentation module (New England Biolabs) for 20 min and 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Benzonase was inactivated using 50 mM EDTA and DNA was then extracted using Monarch Genomic DNA purification kit (New England Biolabs, Ipswich, MA, USA, #T3010S).
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bioRxiv - Synthetic Biology 2023Quote: ... NEBNext ULTRA II FS DNA Library Prep (New England Biolabs, Ipswich, MA, USA, #E6177) was used ...
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bioRxiv - Synthetic Biology 2023Quote: ... The inserted barcoding sequences were annealed using randomized overlapping single stranded oligonucleotides by adding 3 µl of each oligonucleotide (100 µM) to 500 µl 1x 2.1 NEB-buffer (New England Biolabs, Ipswich, MA, USA, #B6002S) followed by boiling at 100 °C for 3 minutes to finally let them associate during the cooling to room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: The targeted intermediate bacmids pBWH-C5-pIX-Kan or pBWH-fC5-E1Kn were digested by SwaI (New England Biolabs, Ipswich, MA, USA, #R0604S) overnight and heat inactivated at 65 °C after which these preparations were used as vectors in the genomic assembly reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng purified linear vector fragments were assembled with 5 µl of annealed oligonucleotide pool using NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA, #E2621L) according to the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (New England Biolabs). Cloning was performed for eight gRNAs at a time ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli culture carrying the gRNA plasmids was cultivated for ∼12 hours in LB medium before plasmids were extracted via mini-prep (New England Biolabs).
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB10beta (New England Biolabs, Ipswich, MA, USA, #C3020K) were transformed by 4 µl of dialyzed bacmid assemblies according to the manufacturer instructions and plated on chloramphenicol (25 µg/ml)-containing LB-agar plates and incubated overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). The quality of the libraries was assessed using the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Synthetic Biology 2023Quote: NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for all assembly reactions ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and BamHI (New England Biolabs, Cat# R3136S) and inserted into the corresponding site of the pLVX-EF1alpha-IRES-Puro vector (PMID ...
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bioRxiv - Microbiology 2023Quote: ... 6 µL of Proteinase K (New England Biolabs) was added to the cell resuspension ...
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bioRxiv - Molecular Biology 2023Quote: ... or HiFi DNA Assembly (New England Biolabs #E2621L) and incubated for 1 hour at 50°C ...
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bioRxiv - Microbiology 2023Quote: ... 3 to 5 ng of RNA from input and corresponding m6A-IPs were used for NGS library production following the protocol NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs), treating samples as rRNA-depleted and fragmented RNAs ...
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bioRxiv - Microbiology 2023Quote: ... was generated using primers 2238–2027 The RNAs were synthesized in 50 μl reactions containing T7 RNA polymerase (25 units; New England Biolabs), 40 mM Tris–HCl (pH 7.9) ...
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bioRxiv - Molecular Biology 2023Quote: ... and a kappa mid-fragment using Gibson Master Mix (New England Biolabs #E2611L) or HiFi DNA Assembly (New England Biolabs #E2621L ...
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Microbiology 2023Quote: ... Spike chimeras were generated through Gibson assembly of PCR generated fragments (NEB). Primers used for site directed mutagenesis and Gibson assembly are described in table 1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... All of the constructs were cloned via Gibson assembly (New England Biolabs, part #E2611S). Primers used in this study were designed with the J5 algorithm and are listed in Table S3.74
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bioRxiv - Synthetic Biology 2023Quote: ... The transcripts were digested with Turbo DNAse (New England Biolabs) at 37 °C for 20 minutes before being gel purified from a denaturing 10% polyacrylamide gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... Q5® High-Fidelity 2X Master Mix (NEB) was used for all polymerase chain reactions (PCRs).
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bioRxiv - Synthetic Biology 2023Quote: ... These sequences were ordered as gBlocks from IDT and assembled (NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... These sequences were ordered as gBlocks from IDT and assembled (NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch Plasmid MiniPrep kit (NEB) for isolating template plasmid and assembled plasmid from E ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB 10β (NEB). L-arabinose (A3256-25 g) ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch DNA Gel Extraction kit (NEB), for purifying PCR products products prior to assembly (Note ...
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bioRxiv - Microbiology 2023Quote: Nucleotide substitutions were introduced into the p239SpE3’:K180S hemiviral plasmid by site-directed mutagenesis (New England Biolabs). Mutated p239SpE3’:K180S hemiviral plasmids were digested with SphI-XhoI and joined with the 5’ half of SIVmac239 SpX and SIVmac239 SpX ΔVif ...
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bioRxiv - Microbiology 2023Quote: ... and DNase treatment using DNase I (New England Biolabs, Ipswich, USA). Library preparation and sequencing were performed by the Australian Genome Research Facility (https://www.agrf.org.au/) ...
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bioRxiv - Molecular Biology 2023Quote: ... The VSV plasmid was digested with restriction enzymes KpnI and HpaI (New England Biolabs, NEB), excising a C-terminal part of G ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of poly(A)+ RNA were digested with a nucleoside digestion mix (New England Biolabs) at 37°C for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.25X T4 RNA ligase buffer (NEB) and resuspended in 5’ adapter mix (10 pmol 5’ adapter ...
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bioRxiv - Molecular Biology 2023Quote: ChIP-seq libraries were prepared with 2 ng of input or IP DNA using the NEBNext Ultra II DNA Library kit for Illumina (New England Biolabs). The quality of the libraries was assessed using the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Synthetic Biology 2023Quote: ... OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (New England BioLabs, Cat.#M0486) was used ...