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Citations for New England Biolabs :
7601 - 7650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Poly(A)+ RNA was further isolated using double selection with poly-dT beads (E7490, NEB). RNA-seq libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (E7760 ...
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bioRxiv - Genomics 2023Quote: ... The resulting digested DNA (4 ml in total) was divided into four aliquots and diluted in 8 ml of ligation buffer (1X ligation buffer NEB without ATP ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21(DE3) strain (C2527I, New England Biolabs) and grown in M9 medium for 15N labeling ...
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bioRxiv - Cell Biology 2023Quote: ... and ligated using T4 DNA Ligase (M0202, New England Biolabs) to the pET vector digested with the same restriction enzymes ...
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bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
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bioRxiv - Biochemistry 2023Quote: ... and carried out using the KLD enzyme mix (NEB) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were amplified by PCR with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs #174M0541S) for 9 cycles in 96-well Thermal cycler (Biorad ...
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bioRxiv - Microbiology 2023Quote: ... This product was then digested with the respective restriction endonuclease enzymes (New England Biolabs) listed in S4 Table ...
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bioRxiv - Microbiology 2023Quote: ... and used to prepare the library with the Next ultra II DNA library prep kit (E7645, NEB) using 1 μg of DNA per sample as input ...
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bioRxiv - Microbiology 2023Quote: ... Linear HDR templates for knockout were PCR amplified using Phusion DNA polymerase (New England Biolabs) with T7 Forward and M13 Reverse primers ...
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bioRxiv - Genomics 2023Quote: ... 10 µg total RNA or 15 µL of polysomal RNA (half of 30 µL eluate, volumetric inputs) was treated with DNaseI (NEB, M0303) in a 100 µL reaction at 37 °C for 15 min ...
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bioRxiv - Genomics 2023Quote: 1-2 µg of extracted total RNA and polysomal RNA was treated with DNaseI (NEB, M0303) in a 100 µL reaction at 37 °C for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... and UP* in the list below) were generated at a concentration of 40 μM in NEBuffer#2.1 (NEB) buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The templates were then incubated with 50 units of exo-Klenow enzyme (NEB) and 1 mM dNTP with 0.35 mM DIG-dUTP (biorbyt ...
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bioRxiv - Biochemistry 2023Quote: ... Broad Range (10-250 kDa) (New England Biolabs #P7719S) was used as a protein standard ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs were performed using Phusion DNA polymerase (New England Biolabs) and the supplied ‘GC buffer’ under manufacturer-recommended conditions ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain NEB 10-beta (New England Biolabs) was used for all cloning ...
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bioRxiv - Neuroscience 2023Quote: ... C-sbGluc and p2a dTomato by Gibson cloning (New England Biolabs HiFi DNA Master Mix). Coding sequences from plasmids for GCaMP6f ...
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bioRxiv - Molecular Biology 2023Quote: The cloning procedures were performed as previously described (Düsterhöft et al., 2021): NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) was utilised to produce different plasmids with the desired inserts according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... was followed by cDNA synthesis (NEB, Invitrogen) and quantification of relative mRNA levels using a LightCycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... which were then inserted using NEBuilder® HiFi DNA Assembly kit (NEB). The expression cassette for WT or mutant RAD6 in the pRS41H backbone was excised with Kpn1 and Sac1 and inserted into the same sites in vector pRS305 (LEU2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The LGE1 promoter and terminator amplicons were then inserted into Not1-Xho1-digested vector pRS304 (TRP1, CEN)49using NEBuilder® HiFi DNA Assembly (NEB) to obtain pMC12 ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: Purified vector populations from input and output samples were linearized by addition of 24 µL of SacI restriction enzyme (NEB R0156L) with 16 µL of NEB r1.1 buffer and overnight incubation at 37 °C ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... A NotI-HF (NEB R3189S) and AflII (NEB R0520S ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and AflII (NEB R0520S) double digest was performed on the four now recombined eluates ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase inhibitor (Biolabs, 40,000U/ml)) ...
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bioRxiv - Cell Biology 2023Quote: ... The sequences were cloned into the pUASz1.0 vector (54) with the eGFP ORF at the C-terminal end of the Top3β ORF using a ligation kit (NEB). The UASz vector allows one to induce the constructs in any tissue ...
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bioRxiv - Cell Biology 2023Quote: Protein G magnetic beads were washed 2-3x with 1ml blocking buffer (20 mM Hepes, 150 mM KCl, 20% Glycerol, 0.5 % Tween, BSA (Biolabs), Heparin 0.2 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase inhibitor (Biolabs, 40,000U/ml)) ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Assembled arrays were preserved through ligation with T4 DNA ligase and its corresponding buffer (NEB M0202L) for 1.5 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA) was used per manufacturer’s protocols to assemble the expression plasmid encoding lanM ...
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bioRxiv - Microbiology 2023Quote: ... coli competent cells (New England Biolabs, Ipswich, MA, USA) ...
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bioRxiv - Genomics 2023Quote: ... Commercial and breeding program samples were extracted from frozen tissue using the Monarch Genomic DNA Purification Kit (NEB), as per manufacturer’ s instructions using the enzymatic cleanup protocol with both proteinase K and RNase A ...
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bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37 °C for 20 min.
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bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37°C for 20 min ...
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bioRxiv - Genomics 2023Quote: A final Q5 PCR reaction was carried out for 8 cycles with the same formulation as PCR2 but using NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB, E7600S) according to manufacturer’s recommendations (65 °C annealing) ...
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bioRxiv - Genomics 2023Quote: 1 µg of each library in pDONR223 was digested with Blp1 (NEB) for 1 h 15 min at 37 °C ...
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bioRxiv - Genomics 2023Quote: Purified PCR1 products (landing pad insert) were amplified for each of COMT target regions in a 50 µL NEB Q5 reaction (NEB, M0491) for 8 cycles ...
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bioRxiv - Genomics 2023Quote: Nucleic acids (gDNA, 1st strand cDNA) were amplified with Q5 polymerase (NEB, M0491) in 50 µL PCR reactions for 16 cycles with 500 nM landing-pad-specific primers (pDEST_HC_Rec_Bxb_v2_F/R ...
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bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
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bioRxiv - Cell Biology 2023Quote: ... phosphorylated at 5’-termini by T4 Polynucleotide Kinase (M0201, New England Biolabs) and ligated using T4 DNA Ligase (M0202 ...
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bioRxiv - Biochemistry 2023Quote: ... Primers for generating mutations for MsbA were designed using the online tool NEBaseChanger (NEB) and carried out using the KLD enzyme mix (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... and at 37 °C for MscI (New England Biolabs, R0534) and BstEII-HF (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... or with 20 U each of KpnI-HF and SpeI-HF (New England Biolabs, R3142 and R3133) at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... with pUC19 (New England BioLabs, N3041S) used as filler to ensure efficient transfection ...
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bioRxiv - Bioengineering 2023Quote: ... and BstEII-HF (New England Biolabs, R3162).
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bioRxiv - Bioengineering 2023Quote: ... Double-stranded DNA was synthesized by Integrated DNA Technologies and inserted into pAAV backbones with NEBuilder HIFI (New England Biolabs, E2621). sgRNA sequences were synthesized as overlapping single-stranded DNA oligos (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... at 25 °C for SmaI (New England Biolabs, R0141), and at 37 °C for MscI (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... sgRNA sequences were synthesized as overlapping single-stranded DNA oligos (Integrated DNA Technologies) that were then annealed together and ligated into sgRNA expression cassettes using T4 DNA ligase (New England Biolabs, M0202).