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Citations for New England Biolabs :
7551 - 7600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were run alongside 5 μL of 1kb Plus DNA Ladder (New England Biolabs®) or pHAPE ladder [24] and separated at 90V (TAE ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared using a polyA selection method using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA) and quantified using Qubit 4.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... was digested with XbaI(New England BioLabs, Japan) and used as a template for in vitro transcription with MEGAshortscript T7 kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Plugs were then equilibrated twice for 30 min at room temperature in 1 ml of 1x NEBuffer 3.1 (New England Biolabs). After the buffer was completely discarded ...
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bioRxiv - Neuroscience 2023Quote: ... The recipient vector was dephosphorylated with shrimp alkaline phosphatase (rSAP NEB #M0371S) during the digest ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3 μL USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Microbiology 2023Quote: ... the product was transformed into DH5-α cells (NEB, Cat. No. C2987H). Following culture on LB-ampicillin plates (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... dephosphorylated using rSAP (NEB, Cat No. R0146L), and amplified by PCR using primers that extend outward from the extremes of barcode region (Forward ...
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bioRxiv - Microbiology 2023Quote: ... A second PCR purification was performed prior to insertion of the Ultramers into the vector by DNA assembly with the NEBuilder HiFi DNA Assembly Kit (NEB, Cat. No. E2621). Next ...
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bioRxiv - Microbiology 2023Quote: ... samples were amplified using 0.5 units of Q5 high-fidelity DNA polymerase (NEB #M0491S) in 25 μl reaction with 1X Q5 reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... using Gibson assembly (New England Biolabs, Frankfurt, Germany). The resulting vector was transformed into chemically competent E ...
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bioRxiv - Microbiology 2023Quote: ... and 1 unit/µL of T4 DNA Ligase (NEB #M0202). A no-insert with backbone only control was prepared in parallel with each ligation reaction to serve as a backbone self-ligation control ...
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bioRxiv - Microbiology 2023Quote: ... Gibson Assembly® Master Mix (NEB #E2611) was used for all Gibson assembly reactions following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... each plug was incubated in 160 μl of 1x NEBuffer 3.1 containing 1 unit/μl of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was clarified by centrifugation at 5°C at 18 500 rpm for 30min and loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB1_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... was used as per manufacturer protocol to isolate mRNA for input into the NEBNext® Ultra™ II RNA Library Prep Kit (New England Biolabs) using NEBNext Unique Dual Index Primers (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... The genes were subcloned into pFastBac using NEBuilder HiFi DNA assembly (NEB E2621S). zz-TEV-Halo-hBicD2FL-6xHis in donor vector pKL for expression in insect cells and zz-TEV-Halo-hBicD2(1-598 ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons (approximately 3.4 kb) were gel purified using Monarch DNA gel extraction kit (NEB). Library construction ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 U/µl Klenow exo (-) Fragment (NEB, #M0212L). The random priming site serves as a UMI in downstream analysis.
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bioRxiv - Systems Biology 2023Quote: ... 16 mU/uL proteinase K (NEB, P8107S)) ...
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bioRxiv - Systems Biology 2023Quote: ... then incubated overnight with DraI mix (2 U/uL DraI enzyme (NEB, R0129) in 1X CutSmart buffer ...
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bioRxiv - Systems Biology 2023Quote: ... the flow cell was incubated with Exonuclease I mix (1 U/uL Exo I (NEB, M0293L) in 1x Exo I buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) was used as per manufacturer protocol to isolate mRNA for input into the NEBNext® Ultra™ II RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 µg of the lysate was digested with 1000 U micrococcal nuclease (NEB, Cat. # M0247S) for 30 min at 22°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PURExpress® kit containing Solution A and Solution B (NEB) was used to prepare the transcription-translation reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... The lipid film was rehydrated by vortexing with 21 μL rehydration solution (14 μL PURExpress Solution A, 0.875 μL RNase Inhibitor (NEB), 17 μM IGEPAL (90mM) ...
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bioRxiv - Cell Biology 2023Quote: ... using NEBNext Unique Dual Index Primers (New England Biolabs). Library preparation was conducted as per manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... DNA libraries were produced using NEBNext Ultra II DNA Library Preparation kit and NEBNext Unique Dual Index Primers (New England Biolabs). Libraries were quality checked using Agilent D1000 TapeStation kit and Qubit Flourometer and then sequenced on the Illumina NovaSeq X (Admera Health ...
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bioRxiv - Systems Biology 2023Quote: ... The mix contains 1x NEBuffer-2 (NEB, #B7002S), 10 µM of Randomer (custom DNA oligo ...
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bioRxiv - Zoology 2023Quote: ... and 0.5 µL of RNAsin (New England Biolabs, Massachusetts, USA), resulting in a total volume of 20 µL ...
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bioRxiv - Cell Biology 2023Quote: ... and inserting it by Gibson Assembly (New England Biolabs) into the BglII/NotI sites of a customized cloning vector bearing an N-terminal Halo-tag (pENTR20-Halo-C1) ...
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bioRxiv - Systems Biology 2023Quote: ... PCR with custom Nextera PCR primers was then performed for five cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Ipswich, MA, USA) 76 ...
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bioRxiv - Genetics 2023Quote: ... 1.2 M sorbitol) and resuspended in digestion buffer (Buffer B, 200 mM Vanadyl ribonucleoside complex [VRC from NEB] ...
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bioRxiv - Cell Biology 2023Quote: ... XbaI (NEB, USA) and BamHI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... and BamHI (NEB, USA). Three DNA fragments of Kozak-3HA ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on mEGFP-ATP7B were prepared following Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The gel was then digested with proteinase K (NEB, cat. no. P8107S) at a final concentration of 8 U/ml in digestion buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... the frequent cutter Nt.CviPII nickase (recognition site: CCD; New England Biolabs Inc., Ipswich, Massachusetts, USA) and DNase I were applied after the washing steps following permeabilization ...
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bioRxiv - Microbiology 2023Quote: ... The catalytic residue H528 of esaD was mutated to encode an alanine using a Q5 site-directed mutagenesis kit (NEB). esxCBED amplified from COL genomic DNA was then inserted upstream of esaD by HiFi assembly ...
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bioRxiv - Neuroscience 2023Quote: ... Up to 10 ng of gDNA was amplified in a 20 μl volume using NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) supplemented with 5% dimethyl sulfoxide and barcoded primers specific to HTT Exon 1 (0.5 μM each ...
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bioRxiv - Genomics 2023Quote: ... using Klenow fragment (NEB) and re-ligated ...
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bioRxiv - Genomics 2023Quote: ... After overnight-digestion with HindIII (NEB), DNA-ends were labeled with Biotin-14-DCTP (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... at 10 pmol each and then amplified in 9 cycles using Phusion DNA polymerase (New England Biolabs) from primers that added flanking overlaps to tiles allowing Infusion cloning in a screening vector ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were double digested with HindIII-HF (NEB, #R3104S) and KpnI-HF (NEB ...
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bioRxiv - Genetics 2023Quote: ... and KpnI-HF (NEB, #R3142S) restriction enzymes ...
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bioRxiv - Immunology 2023Quote: ... and subsequently modified by the addition of a 7-methylguanosine cap with the Vaccinia Capping System (New England Biolabs [NEB]) using the NEB Capping protocol (NEB ...
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Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strainbioRxiv - Molecular Biology 2023Quote: ... This plasmid was cut with BaeI and a guide sequence DNA targeting the genomic region encoding Hsh155 HRs 15-16 region (Table S1) was inserted using HiFi DNA Assembly (New England BioLabs) to make p416-TEF1p-Cas9-NLS-crRNA-HSH155.
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bioRxiv - Molecular Biology 2023Quote: ... 40 μl of 5 pM λ-phage DNA (New England Biolabs), digoxigenin-labelled at both ends14 ...