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Citations for New England Biolabs :
501 - 550 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: The dual pegRNA lentiviral vectors with pre-integrated co-selection pegRNAs were prepared for pegRNA library cloning by BsmBI-digestion and Quick CIP (#M0525L, NEB) treatment followed by gel purification ...
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bioRxiv - Molecular Biology 2023Quote: ... Type I-F Cascade was co-expressed with 6xHis-MBP-TEV-TniQ and a type III-B crRNA in NiCo21 cells (NEB). Cells were then induced with 0.5 mM isopropyl at 18 °C for another 18–20 hours before harvesting ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally hexahistidine-tagged Sfr1 was co-expressed with Swi5 as an operon from a pET11b vector in an Escherichia coli BL21 DE3 strain (NEB) that had been pre-transformed with the pRARE2 plasmid (Novagen) ...
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bioRxiv - Microbiology 2023Quote: ... RHCas9 Δgra72 parasites were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus and SacI (New England Biolabs)-linearized pTwist-CMV: ...
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bioRxiv - Microbiology 2023Quote: ... RH-Luc+ Δgra47 or ME49-cLuc+ Δgra47 were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus (Table S1) and EcoRV (New England Biolabs)-linearized pUPRT::GRA47HA plasmid at a ratio 1:5 of sgRNAs to linearized plasmid ...
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bioRxiv - Microbiology 2020Quote: ... RNA extracts were treated with RNase-Free DNase I enzyme (New England Biolabs). Complete removal of gDNA was confirmed by PCR using gapA-check-F/R primers (Supporting Table 3) ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was resuspended in 10 μl of nuclease free water (New England Biolabs). 5 μl of RNA solution was suspended in 5 μl of RNA loading buffer (95% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Crude RNA extracts were treated with RNase-Free DNase I (New England Biolabs). Complete removal of gDNA was confirmed by PCR using gapA-check-F/R primers (Dataset S1c) ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated with 10 U of phosphatase-free T4 polynucleotide kinase (New England BioLabs) for 30 min at 37°C ...
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bioRxiv - Physiology 2020Quote: ... Template DNA was then digested with RNase free DNase (NEB, Hitchin, Hertfordshire, UK). RNA probes were stored in 25% formamide/2x saline-sodium citrate (25% FA/2x SSC ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µl Reverse External Primer (10 µM) and Nuclease-free water (NEB,USA) up to 100 µl were mixed on ice ...
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bioRxiv - Genomics 2021Quote: ... In step 5 extra free oligo was removed by Thermolabile Exonuclease I (NEB). After adding 50 ng of carrier DNA (poly (A) ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of nuclease free water and 2 μl of DnaseI (M0303S, NEB) were added to degrade the DNA template ...
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bioRxiv - Molecular Biology 2022Quote: ... DNAse I digestion was carried out using RNAse free DNase I (M0303S- NEB), and reverse transcription on 1 μg of DNase treated total RNA was performed using RevertAid Reverse Transcriptase ...
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bioRxiv - Neuroscience 2022Quote: RNA solution was then treated with DNase I (RNase-free) (New England Biolabs) to remove genomic DNA from sample and protected against RNA degradation using RNasin® Ribonuclease Inhibitor (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... These plasmids (Table S6) in combination with the cell-free system PURExpress (NEB) were used to produce bacteriocins in vitro ...
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bioRxiv - Immunology 2024Quote: ... isolated RNA samples were treated with RNase-free DNase I (New England Biolabs) to remove any contaminant DNAs which might yield false positives in downstream PCR tests ...
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bioRxiv - Microbiology 2023Quote: ... gDNA-free RNA was purified using Monarch RNA Cleanup Kit (New England Biolabs) and visualised on an agarose gel.
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bioRxiv - Microbiology 2023Quote: ... RNA extracts were treated with RNase-Free DNase I enzyme (New England Biolabs). Complete removal of gDNA was confirmed by PCR using gapA-check-F/R primers (Supporting Table 2) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of nuclease-free water and 5 µL of GA mastermix (NEB) were added and incubated at 40°C for a minimum of 1.5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The total RNA was further treated with RNase-free DNase I (NEB, M0303) and reverse-transcribed using the random hexamer and Superscript II (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... The TRIzol-isolated RNA was treated with 10 U RNase-free DNaseI (NEB) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclease free water and 1X Phi 29 reaction buffer (New England Biolabs, B0269SVIAL) were added to each well followed by incubation at 70°C for 3 mins ...
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bioRxiv - Microbiology 2024Quote: ... DNA-free RNA was used for cDNA synthesis using random nonamers (N9; NEB) and SuperScript III reverse transcriptase (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... dissolved in nuclease-free water and treated with DNase I (New England Biolabs). RNA was then re-extracted using phenol/chloroform and re-precipitated with 99.5% ethanol and 0.3 M sodium acetate ...
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bioRxiv - Microbiology 2024Quote: ... Restriction free cloning was conducted using NEBuilder® HiFi DNA Assembly (NEB: E2621L). Isolation of plasmids and purification of PCR products were conducted by using commercially available kits from either Qiagen or Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... The amplicons were eluted with 16 µL nuclease-free water (New England Biolabs) and vortexed thoroughly ...
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bioRxiv - Genomics 2024Quote: ... PCR-free DNA libraries were prepared with the NEBNext Ultra II kit (NEB). The libraries were multiplexed and sequenced on the Illumina NovaSeq 6000 and MiSeq sequencers at Okinawa Institute of Science and Technology Graduate University and Pennsylvania State University (Table S1) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... purified proteins were directly digested by O-glycosidase (P0733, NEB, 4,000 units/µg protein) and α2-3 ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µg DNA was treated with uracil DNA glycosylase (New England Biolabs, Inc.) at 37 °C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 µg of total RNA was decapped with mRNA decapping enzyme (New England Biolabs) for 1 hour at 37 ºC ...
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bioRxiv - Biophysics 2021Quote: ... we linearized 40 µg of the vector with NotI (R0189S, New England Biolabs, MA), mixed the linearized plasmid with 5×107 cells that we had washed with 10 mL of cytomix (120 mM KCl ...
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bioRxiv - Genetics 2020Quote: ... 60 µg of this 3C-extract were digested with 100 units NcoI-HF (NEB) over night ...
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bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Molecular Biology 2023Quote: ... A total of 6-12 µg of genomic DNA was dephosphorylated with rSAP (NEB), followed by AMPure XP beads purification ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of genomic DNA from the clones were digested solely with XhoI (NEB) overnight at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Immunology 2022Quote: ... cleaned and concentrated 20x using the Monarch PCR & DNA Cleanup Kit (5 µg) (NEB). Further cleanup was done with the E-gel imager system (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 µg of DNA was incubated with 0.1 U/µL of USER enzyme (NEB) in 500 µL at 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... 5 µg of sequencing verified plasmid were restriction digested using BsmBI V2 (NEB, #R0739S) in a 50 µl reaction with NEB3.1 buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified using a 10 µg Monarch RNA Cleanup Column (New England Biolabs) and eluted in 42 µl of nuclease-free water.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified using a 50 µg Monarch RNA Cleanup Column (New England Biolabs), eluted in 20 µl of nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified using a 10 µg Monarch RNA Cleanup Column (New England Biolabs) and eluted in 10 µl of nuclease-free water.
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bioRxiv - Molecular Biology 2024Quote: ... 16 µg purified RNA was mixed with 6X gel loading dye (New England BioLabs) and run on a 1% agarose-TAE (20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... pUber was digest with BstXI (2.5 Units per µg of DNA, New England Biolabs) in NEBuffer 3.1 for 8 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was subsequently purified using the Monarch RNA Cleanup Kit (50 µg) (#T2040, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled RNA was purified using a Monarch RNA Cleanup Kit (10 µg, NEB).