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Citations for New England Biolabs :
7151 - 7200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... DNA was eluted in 17μl of water and further 3’ A-tailed using 2.5 units of Klenow 3’ to 5’ exo(-) (NEB, cat M0212) in 1X NEB buffer 2 supplemented with 0.2 mM dATP for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... m6A-immunoprecipitation (m6A-IP) were performed using the monoclonal m6A antibody from the EpiMark N6-Methyladenosine enrichment kit (NEB cat. E1610S). Input and eluted total RNA from m6A-IP were used to prepare libraries with Takara Pico-Input Strand-Specific Total RNA-seq for Illumina v2 (Takara) ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) according to the manufacturer’s instructions and the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... and decapped with 10 U of T4 PNK +/− 40 nmol of ATP and 5 U of RppH (NEB). After each step ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5μl water and 1.5 ml of NEB Quick ligase (NEB, M2200). The reaction was incubated 20 minutes at room temperature and 5μl of 0.5M EDTA was added to inactivate the ligase enzyme ...
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bioRxiv - Molecular Biology 2023Quote: Radioactive labelling of the in-vitro-transcribed RNA was carried out by first dephosphorylating 50 pmol of RNA with 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were first treated with End repair enzyme mix (NEB, E5060) for 30 min at 20°C in 50μl volume ...
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bioRxiv - Molecular Biology 2023Quote: ... was dephosphorylated with 3μL of Quick CIP enzyme (NEB, M0508) for 10min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... For the library preparation of the pools the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich (MA) – United States of America ...
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bioRxiv - Molecular Biology 2023Quote: 5μg of input DNA in 30μL of 1X CutSmart buffer (NEB, B7204), was dephosphorylated with 3μL of Quick CIP enzyme (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and concentrated using a Monarch RNA clean-up kit (NEB, T2040S). The IVT gRNAs were either kept on ice for use the same day or aliquoted and snap-frozen in liquid nitrogen.
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bioRxiv - Molecular Biology 2023Quote: ... The 500bp PCR product was digested with NspI (New England Biolabs, R0602L) that recognizes a RCATG/Y cut site only present in mutants ...
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bioRxiv - Molecular Biology 2023Quote: ... NbALFA-LgBiT and LgBiT-NbALFA were amplified by PCR and cloned in pcDNA3.1+ using NEB HiFi DNA Assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... βArrestin2-ALFA-GFP2 were amplified by PCR and cloned in pcDNA3.1+ using NEB HiFi DNA Assembly (New England Biolabs). Gβ3 and Gγ2-GFP2 were generously gifted by Dr ...
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bioRxiv - Molecular Biology 2023Quote: ... and subsequently DNA was purified using Monarch PCR & DNA Cleanup Kit (New England BioLabs, #T1030). DNA fragments were amplified with PCR and samples were barcoded using published primers (36) ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, #E7645L) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, #E7765) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μM dNTP Solution Mix (New England Biolabs), and a concentration of biotin-11-dNTPs (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μl PCRs contained 1X ThermoPol Buffer (New England Biolabs), 250 nM PRA1_NoMod.F or PRA1_shrt.F ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed using the NEBNext Ultra II DNA library preparation kit for Illumina according to the manufacturer’s instructions (NEB E7645S). To determine the number of PCR cycles required for amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomal-depleted RNA was then used for library preparation using NEBNext Ultra II Directional RNA Library Prep kit (NEW ENGLAND BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was amplified using standard PCR using LongAmp Taq DNA polymerase (NEB) targeting the CRISPR locus with a 200-500 bp long amplicon ...
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bioRxiv - Molecular Biology 2023Quote: ... Barcodes were amplified with 22 cycles of PCR using Phusion polymerase (NEB) with primers that add Illumina adaptor sequences and a 6 bp identifier sequence to distinguish the various drug conditions and time points ...
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bioRxiv - Molecular Biology 2023Quote: ... The sir2 ORF was amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) and cloned into p123 within the NcoI and NotI restriction sites of p123 ...
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bioRxiv - Molecular Biology 2023Quote: ... Deletion for hst6 (UMAG_12006) was performed using the NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA) system ...
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bioRxiv - Molecular Biology 2023Quote: ... All the fragments were cloned into the pBluescript II SK (+) plasmid using the NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA). Constructs were amplified by PCR prior to their transformation in U ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV9-based rep-cap plasmids incorporating individual heptameric peptides (EC1-10) (pACG2-[EC1-10], pACG2-QuadYF+TV-[EC1-10], and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain BL21 (DE3) and subsequently purified with amylose beads (NEB). The RNA affinity purification of mRNA 3’ processing complex was carried out essentially as described before (15) ...
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bioRxiv - Molecular Biology 2023Quote: ... with 1x GlycoBuffer 1 (NEB). Reactions were performed on 3μg total RNA from indicated cell sources for 60 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... ddH2O and 2μL of diluted cDNA were used in 10μL qPCR reactions containing the Luna® Universal qPCR Master Mix (NEB) and 0.3μM of gene-specific forward and reverse primer ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was carried out with phusion polymerase (New England Biolabs) according to instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... We blunted the resulting sticky ends using Klenow end-blunting (NEB) and re-circularized the plasmid using T4 blunt-end ligation (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Targeted RNase H (NEB) digestions were performed according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... pull-down was performed with 100 μl pre-blocked (NETS buffer with 4 mg/ml BSA (NEB) and 2 mg/ml tRNA (SigmaAldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... added to 200 μl of 2.5% (v/v) of MagStrep “type3” XT beads (IBA Lifescience) that has been blocked overnight with 1 mg/ml BSA (NEB) and 0.5 mg/ml tRNA (SigmaAlrich ...
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bioRxiv - Molecular Biology 2023Quote: ... After column purification with RNA monach kit (NEB), the concentration of RNA transcripts were determined by UV absorbance ...
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bioRxiv - Molecular Biology 2023Quote: ... to the branch point recognition sequence (Nucleotides 18-42) of U2 snRNA and to GAPDH mRNA were added in combination with RNase H (NEB) to NE ...
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bioRxiv - Molecular Biology 2023Quote: ... the binding reaction was added to 200 μl of 5% (v/v) amylose beads (NEB) that has been blocked overnight with 1 mg/ml BSA and 0.5 mg/ml tRNA in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... containing an N-terminal 6xHis tag and TEV site was produced in Escherichia coli NiCo21 (DE3) cells (New England Biolabs) as previously described in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069193/ ...
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bioRxiv - Molecular Biology 2023Quote: ... using a PvuII (NEB) -linearized plasmid standard ...
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bioRxiv - Molecular Biology 2023Quote: ... The AAV transfer vectors used for 3-plex sgRNA delivery into skeletal muscle were cloned between AAV serotype 2 ITR’s including a cloning site for multiplexed hU6-sgRNA insertions (MluI and KpnI (NEB)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 pmol of this RNA was 5’-end-labelled (20 µCi of 32P-γATP) using 1 U of polynucleotide kinase (NEB) at 37°C for 1 h in a 20 µL reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries for next-generation sequencing were prepared using the NEBNext Ultra(tm) II Directional RNA Library Prep kit with Sample Purification Beads (NEB, E7765S). Libraries were indexed using NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 2 (E7335S and E7500S) ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina Truseq adaptors were ligated to the DNA fragments by adding 25μl of 2X Quick Ligase buffer (NEB, M2200), 1μl of adaptors (1μl of a 1:250 dilution of Illumina stock solution) ...
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bioRxiv - Molecular Biology 2023Quote: ... Poly(A) tail selection was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S) and libraries for next-generation sequencing were prepared using the NEBNext Ultra(tm ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were fragmented at 94°C for 2.75 min as per the NEBNext Magnesium RNA Fragmentation Module (NEB) with subsequent RNA purification using the Zymo RNA Clean & Concentrator kit ...