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Citations for New England Biolabs :
7251 - 7300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB). Sequencing was performed on the Illumina HiSeq 2500 using the Rapid Run mode.
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bioRxiv - Neuroscience 2023Quote: ... These two fragments were digested by EcoRI (New England Biolabs, Cat # R0101) and then linked together using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C ...
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bioRxiv - Neuroscience 2023Quote: ... by using the SapI restriction enzyme (BioLabs, #R0569). To confirm the successful insertion of sgRNAs into the plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 (DE3) (New England Biolabs, cat#C2527H). Positive clones were screened by colony PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed according to NEBNext Small RNA Library Prep kit (NEB) protocol ...
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bioRxiv - Molecular Biology 2023Quote: Purified RNA was digested and dephosphorylated to single nucleosides using nucleoside digestion mix (NEB, M0649S) at 37 °C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA from light and heavy fractions were subjected to qRT-PCR using Luna RT-qPCR kit (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The pool was end-repaired and dA-Tailed using the NEBNext End repair / dA-tailing Module (New England BioLabs, cat#E7546), and purified with 1X Agencourt beads ...
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bioRxiv - Molecular Biology 2023Quote: Purified samples were combined with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs M01541) for amplification ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA samples from empty vector and sgRNA2 MEFs were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1X Thermo Pol (New England Biolabs B9004) Buffers ...
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bioRxiv - Molecular Biology 2023Quote: ... then resuspended in 1X T4 PNK Reaction Buffer (New England Biolabs B0201). Samples were incubated at 37°C for 1 h after the addition of T4 PNK (New England Biolabs M0201 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated at 37°C for 1 h after the addition of T4 PNK (New England Biolabs M0201) to allow 5’-hydroxyl repair ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were generated according to protocols from the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490L) and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs, Evry-Courcouronnes, France) for 3 h at 30 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) and then evenly pooled and sequenced on the Illumina NovaSeq with 150bp paired-end reads and a read dept of at least 20 million reads per sample ...
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bioRxiv - Neuroscience 2023Quote: ... and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... PCR fragments and linearized pDIA6754 (31) were mixed and assembled using Gibson Assembly (NEB, France) and transformed by heat shock in E ...
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bioRxiv - Microbiology 2023Quote: ... Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Phage genes were amplified from Butters genomic DNA by PCR using Q5 high-fidelity DNA polymerase (New England Biolabs). PCR products were digested with XbaI overnight (O/N) ...
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bioRxiv - Microbiology 2023Quote: RNA samples were depleted for ribosomal RNA using the NEBNext Bacteria rRNA Depletion Kit (New England Biolabs, Ipswich, Massachusetts, USA). Samples were pooled into a library using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2023Quote: ... or deglycosylated by an 1h treatment with PNGase F (NEB) then eluted in 2X Laemmli as previously described.
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bioRxiv - Microbiology 2023Quote: ... 1 unit of Phusion Polymerase were mixed with 1x HF Phusion buffer (NEB) and made up to 50 µl total volume with H20 ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... and tasA_downstream_rv and the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... The eluted DNA was used for sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645). Biotinylated DNA fragments were enriched by Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently fragmented by dsDNA Fragmentase (New England BioLabs, M0348S) at 37°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... with NEBnext Multiplex Oligos for Illumina (Primer Set 1) (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of DNase I (New England BioLabs, M0303S), 3.5 μl of DNase buffer and 1 μl of RNase inhibitor were added ...
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bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... 12.5 µl of One Taq Quick-load 2x master mix with standard buffer (New England BioLabs, UK) was aliquoted into a DNase-free 0.2ml transparent PCR tube ...
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bioRxiv - Microbiology 2023Quote: ... at 37 °C or Bt.NstNBI nicking endonuclease (NEB) at 55 °C for 1 hour ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gibson Assembly® Master Mix (NEB), Ampicillin (BRAND) ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared using the NEBNext Ultra II Library Prep Kit for Illumina (New England Biolabs, E7645) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA ends were repaired using NEBNext Ultra II Library Prep (New England Biolabs, E7645) reagents and methylated adapters were annealed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Bisulfite treated DNA was amplified using NEBNext index primers (NEB, E7335) and KAPA HiFi Uracil+ mastermix (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Amylose sepharose beads (New England Biolabs)) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was sheared by incubating the nuclei in 100 µl of buffer B supplemented with 1,000 units of micrococcal nuclease (M0247S; NEB) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: PCR of genomic DNA was performed using LongAmp Taq DNA polymerase (NEB) and primers designed against an amplicon of 200-500 bp targeting the CRISPR-locus and the top four CRISPOR-predicted off-targets ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (NEB) for the following protein expression steps.
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bioRxiv - Microbiology 2023Quote: ... enzymes (New England Biolabs) were incubated with cell lysates for 18 h at 37°C prior to protein reduction ...
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bioRxiv - Microbiology 2023Quote: ... Fragments were gel purified and enzymatically digested with SmlI (NEB) for 1h at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... and libraries for deep sequencing were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Quality of DNA libraries was validated using a 2100 Bioanalyzer Instrument (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... and PNGase F (New England Biolabs) (1:100=enzyme ...
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bioRxiv - Neuroscience 2023Quote: The assay was carried out following the manufacturer’s instructions (NEB, P0753S). Details are given in SI Appendix.
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bioRxiv - Neuroscience 2023Quote: ... the cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and were assessed using Qubit 2.0 fluorometer to calculate the concentrations and Bioanalyzer Instrument to determine insert size ...