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Citations for New England Biolabs :
7201 - 7250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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bioRxiv - Molecular Biology 2023Quote: ... the duplexes were ligated with the purified vector with T4 DNA ligase (NEB). The ligation products were transformed into DH5α bacteria for amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... coli poly (A) polymerase (New England Biolabs® Inc., Ipswich, MA, USA), and the reaction mixture was incubated at 370 C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-amplification of cDNA was carried out using Q5High-Fidelity 2X Master Mix (New England Biolabs® Inc., Ipswich, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid backbone of the linearized vector was run on the 0.8% agarose gel and purified by Monarch DNA Gel Extraction Kit (NEB). The oligos for the inserted sgRNA targeting sequences were synthesized with two overhangs compatible with the linearized vector and complementary to each other ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were then assembled using NEBuilder HiFi DNA Assembly (New England BioLabs).
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bioRxiv - Molecular Biology 2023Quote: ... the pGL3-U6-sgRNA-PGK-puro was linearized with BsaI (NEB) at 37°C for 1.5h ...
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bioRxiv - Molecular Biology 2023Quote: ... and rps10 (ENSDARG00000034897) were amplified by RT- PCR and cloned into pCS2+HA using NEBuilder HiFi DNA Assembly (New England BioLabs). The pCS2+HA was generated by inserting synthetic DNA fragments with HA- tag sequence into pCS2+ via EcoRI/XbaI restriction sites ...
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bioRxiv - Molecular Biology 2023Quote: ... the pGL3 Basic vector and T/F LTR U3R PCR products were digested with KpnI and HindIII (New England Biolabs, Ipswich, MA, USA), individually as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR conditions were followed according to each primer pair as recommended by NEB using pCMV-SMAUG1-HisTEV2xFLAG as the template ...
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bioRxiv - Molecular Biology 2023Quote: ... The lambda protein phosphatase procedure was carried out following the manufacturer’s guidelines (New England Biolabs). The sample was incubated at 30°C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.
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bioRxiv - Molecular Biology 2023Quote: ... Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB). Mutagenic primers were designed using the NEBaseChanger tool and synthesized (IDT) ...
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bioRxiv - Molecular Biology 2023Quote: ... The annealed oligos were phosphorylated using T4 polynucleotide kinase (PNK, New England Biolabs). The phosphorylated and annealed oligos were then diluted in Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2023Quote: ... and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB). Synthetic DNA fragments were ordered from Genewiz and sequences are listed in Supplementary Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested T/F LTR U3R region was cloned into the linearized pGL3 Basic vector/plasmid and ligated using 1 U of T4 DNA ligase (New England Biolabs, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and 10 units T4 PNK enzyme (NEB) at 37 °C for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... the ligation reaction was treated by 10 U of Exonuclease I enzymes (NEB, USA) for 1 hour at 37 °C for degradation of non-circularized library ...
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bioRxiv - Molecular Biology 2023Quote: Circular library preparation was performed in final T4 ligation mixture of 100 μl including 1X T4 DNA ligase buffer (NEB, USA), 1 μM phosphorylated library ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U T4 DNA ligase enzyme (NEB, USA) and milliQ water for 1 hour at 16 °C which was followed by enzyme inactivation for 20 mins at 80 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/μl ET SSB (New England Biolabs), 3 mM MnCl2 (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 12.5 U of RNase If (New England Biolabs) were added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... such that the final concentration of components in the 20 μl PCR were: 1X Q5 Reaction Buffer (New England Biolabs), 1X Q5 High GC Enhancer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.25 U of RNase H (New England Biolabs) and 12.5 U of RNase If (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X Q5 High GC Enhancer (New England Biolabs), 200 μM dNTP Solution Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 1 μl of a 4-fold dilution of Low Range ssRNA Ladder (New England Biolabs) and 0.75 pmol each of RPIX_SC1_Bridge ...
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bioRxiv - Molecular Biology 2023Quote: ... increasing volumes of SPRI beads were mixed with 1 μl of a 4-fold dilution of 100 bp DNA ladder (New England Biolabs) and diluted to a final volume of 100 μl with 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: Randomly biotinylated DNA templates for TECprobe-ML experiments were PCR amplified from a 5’ biotinylated linear DNA template using Vent (exo-) DNA polymerase (New England Biolabs) and primers HP4_5bio.R and PRA1_NoMod.F (for ZTP and fluoride templates ...
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bioRxiv - Molecular Biology 2023Quote: ... coli RNA polymerase holoenzyme (New England Biolabs). Single-round in vitro transcription reactions for TECprobe-SL experiments were performed as 60 μl reactions containing the same reagents as TECprobe-ML experiments except that the reactions used a 10 nM template DNA that contains an internal etheno-dA stall site downstream of a Cbe pfl ZTP riboswitch variant in which the poly-U tract was removed ...
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bioRxiv - Molecular Biology 2023Quote: ... Translesion DNA synthesis using Sulfolobus DNA polymerase IV (New England Biolabs) was performed as two 100 μl reactions as described previously48 ...
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bioRxiv - Molecular Biology 2023Quote: 5’ biotinylated DNA that was used for transcription antitermination assays and as a PCR template when preparing modified DNA templates below was PCR amplified from plasmid DNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and primers HP4_5bio.R and PRA1_NoMod.F (for ZTP and fluoride templates ...
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bioRxiv - Molecular Biology 2023Quote: ... is inserted in n-terminal of E2 protein of CHIKV 181/25 structural genes (C-E3-E2-6K-E1) and cloned into pcDNA3.1(+) expression vector using gibson assembly cloning kit (NEB, USA). The resulting plasmid is designated as ChAC-VLP ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-sequencing (RNA-seq) libraries were generated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... pBSK-hst5:eGFP and pBSK-hst6:eGFP were generated using the NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA) system ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 U of Thermostable RNase H (NEB) was added to RNase A digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... multiplex qPCR was performed on a Bio-Rad C1000 Touch Thermal Cycler using Hot Start Taq 2× Master Mix (NEB) with HT_Forward and HT_Reverse primers (IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... 3mL of PBS + 0.01% BSA (NEB B9000S) and 40U/mL of RNAse inhibitor were added to a final volume of 5mL and then immediately centrifuged with a swing bucket rotor at 500g for 5 mins at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were treated with Proteinase K (New England Biolabs P8107), extracted with phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA was resuspended in 10 µM VRA3 adaptor (/5Phos/rGrArUrCrGrUrCrGrGrArCrUrGrUrArGrArArCrUrCrUrGrArArC/3InvdT/) and treated with T4 RNA Ligase I (New England Biolabs M0437) for 2 h at room temperature (22°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and Phusion Polymerase (New England Biolabs M0530). The ProNex Size-Selective Purification System (Promega NG2001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were next resuspended in 1X Thermo Pol Buffer and treated with 2 µL RNA 5’ Pyrophosphohydrolase (New England Biolabs M0356) at 37°C for 1 h to promote decapping of 5’ RNA ends ...
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bioRxiv - Neuroscience 2023Quote: ... and BsrgI (New England Biolabs, Cat # R0575). FLEX-postBirA* was formed by linking digested postBirA* fragment with linearized pAAV-FLEX-GFP vector (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Neuroscience 2023Quote: ... were both digested with KpnI (New England Biolabs, Cat # R3142) and BsrgI (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... and then linked together using T4 DNA ligase (New England Biolabs, Cat # M0202) to form postBirA* ...
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Neuroscience 2023Quote: ... Cas9 enzyme was purchased from NEB. Candidate sgRNA/Cas9 RNPs were complexed and tested by zygote electroporation ...