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Citations for New England Biolabs :
7301 - 7350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 7,000 units of TaqI restriction enzyme (New England Biolabs, Ipswich, MA, USA) were added to 6 μL of the PCR mixture ...
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bioRxiv - Neuroscience 2023Quote: ... ~0.1×1013 M13KO7 Helper Phages (NEB) were added to the culture and let the infection for 45 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Multiplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Neuroscience 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB #E7335, #E7500). 10 ng (TRAP RNA from Tg(Atoh1-Egfp-L10a ...
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bioRxiv - Neuroscience 2023Quote: ... N-glycans were released using N-glycanase PNGase F (1239U/ml, New England BioLabs, Inc. cat no. P0709L) and were fluorescently labelled with 2-aminobenzamide (2-AB ...
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bioRxiv - Neuroscience 2023Quote: ... at −80 °C prior to RNA extraction and library preparation with NEB Next® Ultra™ RNA Library Prep Kit (NEB) by Novogene ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were cloned using the Gibson Cloning Kit (NEB). The pLenti-CAG-EGFP construct was cloned by removing the ires sequence in between the BxtXI and BamHI restriction sites from the backbone construct ...
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bioRxiv - Neuroscience 2023Quote: Tail tip genomic DNA was PCR amplified with ATRX_RC gen F and ATRX_RC gen R primers and digested with FspI (NEB R0135S). FspI cuts the wild-type allele (product sizes ...
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bioRxiv - Neuroscience 2023Quote: ... Both input samples without IP and m1A input samples were used for library construction using an NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., MA, USA). Eluted RNA fragments were converted to cDNA and sequenced or treated to induce partial m1A to m6A conversion before cDNA synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... This was confirmed in N1 heterozygous AtrxR245C/+ females after digestion of the wild-type allele using FspI (NEB R0135S) and gel purification of the uncut mutant band ...
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bioRxiv - Plant Biology 2023Quote: ... cloned in the miniT plasmid (NEB), and the assemble with the Golden gate system using the modules for GFP ...
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bioRxiv - Plant Biology 2023Quote: ... and the purified mRNA was used to construct libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Amplifications were performed using the High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) or the GoTaq G2 polymerase (Promega ...
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bioRxiv - Plant Biology 2023Quote: Illumina library was prepared at GIGA Genomics (University of Liege, Belgium) using NEBNext Ultra II DNA library prep kit (New England BioLabs, US) and libraries were sequenced on the Illumina NextSeq500 sequencer for the generation 10 M of paired-end reads (2 x 150 base pair ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used (New England Biolabs, MA, USA). For sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... The micrococcal nuclease (M0247S; New England Biolabs, Ipswich, MA, USA) reaction was performed at 37°C for 20 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (NEB). LOV-Turbo gene was codon optimized for human via GenSmart™ ...
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bioRxiv - Molecular Biology 2023Quote: ... and 8 units of Bst 2.0 warmstart DNA polymerase (NEB) followed by incubation at 60°C for 2 minutes to extend the strand with EdUTP-containing DNA ...
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bioRxiv - Molecular Biology 2023Quote: The starting library strand (50 pmol) and regeneration hairpin (75 pmol) (Supp. Table 1) were combined in a 1X ligation buffer (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was inserted into a pBlueScript vector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2023Quote: Genetically modified zebrafish lines were created using Cas9 protein (EnGen® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs44 ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary to plasmid transformation of competent bacteria (New England Biolabs, Ipswich, USA), selection of clones ...
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bioRxiv - Molecular Biology 2023Quote: ... calf intestinal alkaline phosphatase and assorted restriction enzymes were all purchased from NEB. Sequence optimized cDNA encoding cytokines and receptors were purchased from GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... in a single tube RNA fragments are sequentially dephosphorylated with T4 PNK (NEB), polyadenylated with E ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were flooded with 1 X ThermoPol Buffer (NEB) for 5 minutes at 25°C to equilibrate and digested with 10U RNase HII for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 lysY/Iq (NEB cat n° C3013). The expression plasmid was transformed via heat-shock followed by selection of clones on LB-Agar plates supplemented with 100 µg/mL carbenicillin ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was dephosphorylated with T4 PNK (NEB) and 3 pmol of this dephosphorylated RNA was ligated to an adenylated adaptor using truncated T4 RNA ligase 2 K277Q (Jonathan Weissman) ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified mIL-12bC197S-His6 in complex with mIL-12Rβ1D1-D2-His6 was subjected to an overnight Caspase-3 and EndoH (New England Biolabs) digest (1/100 w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... p189 was linearized by SphI (NEB). To obtain the GFP-heteroduplex ...
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bioRxiv - Molecular Biology 2023Quote: ... and further digested with ExoIII (NEB) to generate single strand circular DNA ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids and recombination templates were treated with the restriction enzymes BamHI and SalI (New England Biolabs, USA) and ligated with T4 ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... coli Poly(A) Polymerase (New England Biolabs, Ipswich, MA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: MNV plasmids were linearised with NotI and phenol/chloroform extracted before being used for in vitro transcription using the HiScribe™ T7 ARCA mRNA Kit (NEB), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ASV-specific qPCRs were performed with Luna Universal Probe qPCR Master Mix (New England Biolabs, Inc. ...
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bioRxiv - Microbiology 2023Quote: ... followed by second strand synthesis using Phusion DNA Polymerase (NEB). The sequence of the amplicon was determined by Sanger sequencing (Azenta) ...
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bioRxiv - Microbiology 2023Quote: ... Whole mRNAseq libraries were generated using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). The clustering of index-coded samples was then performed on a cBot Cluster Generation System ...
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bioRxiv - Microbiology 2023Quote: The pCC2Fos replicon was digested with ZraI (NEB) and electrophoresed on 1% low melting point agarose (Nippon Gene Co. ...
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bioRxiv - Microbiology 2023Quote: ... 0.25 µl of Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA), and 15.5 µl nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... a fragment including the SARS-CoV-2 RdRp catalytic residue was subcloned into pUC19 (NEB) and the resulting pUC19-RdRp was mutated at the RdRp catalytic residue by inverse PCR using mutation-introducing primers (Supplementary Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... We ordered DNA primers from Integrated DNA Technologies and then amplified PCR products using either Q5 Hot-Start Master Mix (#M0494S, New England Biolabs) or KOD XL (#71087-3 ...
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bioRxiv - Microbiology 2023Quote: ... and Phusion high-fidelity DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... and Phusion high-fidelity DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... Homology arms were cloned via Gibson Assembly into pLBG13 digested with BamHI-HF and SalI-HF restriction enzymes (NEB) using NEBuilder HiFi DNA assembly master mix (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... 200 μg/mL Monarch® RNase A (New England Biolabs; Frankfurt (Main), Germany) ...
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bioRxiv - Microbiology 2023Quote: ... and D9 were amplified by Q5 polymerase (New England Biolabs) using primers indicated in Supplementary Table 3 ...
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bioRxiv - Microbiology 2023Quote: ... T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3 ...