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Citations for New England Biolabs :
7101 - 7150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and A- tailed with the NEBNext dA-Tailing Module (New England Biolabs, #E6053L). After adapters ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... end-repaired with the NEBNext End Repair Module (New England Biolabs, #E6050L) and A- tailed with the NEBNext dA-Tailing Module (New England Biolabs ...
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bioRxiv - Systems Biology 2024Quote: ... 66U/μl truncated T4 ligase 2 and 13U/μl murine RNAse inhibitor (all from NEB, USA)) were added at 25°C for 1h ...
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bioRxiv - Systems Biology 2024Quote: ... and T4 DNA ligase (M0202L, New England Biolabs). The assembled plasmids were transformed into competent cells (C404003 ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids were cloned in house using Gibson Assembly with the NEB Hifi Builder Master Mix (New England Biolabs, Ipswich, MA) and/or contracted (GenScript ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmid E.coli plasmid DNA minipreps were performed using the NEB Monarch Plasmid Miniprep Kit and Protocol (NEB #T1010). Prior to starting the miniprep protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (High Efficiency) (#C3019H, NEB) or Electroporation Protocol (#C3020 ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Electroporation Protocol (#C3020, NEB). For electroporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... of PCR products carrying complementary USER junctions were mixed in a 10 μl reaction containing 0.75 units DpnI (New England Biolabs), 0.75 units USER enzyme (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning fragments were amplified with Phusion® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA) or Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Q5® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA). PCR products were digested with Dpn1 (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... sourced from NEB, and BL21(DE3) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with Dpn1 (NEB, Ipswich, MA, USA) to destroy the original template and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were then digested with 1 µl ExoI and purified using the Monarch DNA Gel Extraction Kit (NEB), eluting in 10 µl of elution buffer (10 mM Tris•Cl pH 7.4) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The addition of an NLS or DST to level 1 and level 2 integrase constructs was performed using PCR-mediated site-directed mutagenesis (NEB Q5 Site-Directed Mutagenesis ...
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bioRxiv - Synthetic Biology 2024Quote: ... and XbaI (NEB R0145), gel-purifying the appropriate fragments ...
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bioRxiv - Synthetic Biology 2024Quote: ... by digesting both plasmids with KpnI (NEB R3142) and XbaI (NEB R0145) ...
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bioRxiv - Synthetic Biology 2024Quote: Enzymes for cloning were procured from NEB. Constructs were assembled by PCR-amplifying the fragments with primers encoding SNPs or larger mismatches (e.g. ...
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bioRxiv - Synthetic Biology 2024Quote: ... The samples were cooled and treated with the restriction endonuclease XhoI (New England Biolabs) to facilitate analysis of recombination products ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.1 μL of the T4 ligase (NEB, M0202) in 1× T4 ligase buffer were incubated in a 384-well PCR cycler (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.1 μL of the respective Type IIS enzyme (NEB), and 0.1 μL of the T4 ligase (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... The oligonucleotides or the resulting amplicons were phosphorylated with T4 polynucleotide kinase (PNK) (NEB) according to the manufacturer guidelines ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were amplified using Q5 DNA polymerase (NEB) or an in-house purified proofreading DNA polymerase ...
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bioRxiv - Synthetic Biology 2024Quote: ... All enzymes were provided by New England Biolabs (NEB, Ipswitch, USA), and the reaction mix and procedure were performed according to Gibson et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... The phosphorylated part amplicon and PCR amplified plasmid were ligated with T4 ligase (NEB) for 2 h at room temperature in a 4:1 ratio according to the manufacturer guidelines ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Level 0 plasmid was amplified with the primer pair SLo0765/SLo0766 followed by DpnI (NEB) digestion prior to ligation (any Level 0 plasmid will do ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The linear template for in vitro transcription (IVT) was generated via PCR using Q5 DNA Polymerase (New England Biolabs) with the forward primer (5’-AGCTATAATACGACTCACTATAAGctcctgggcaacgtgctg-3’ ...
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bioRxiv - Synthetic Biology 2024Quote: ... Synthesized modRNA was column purified and eluted with 60 µL water using the 50 µg Monarch RNA Cleanup Kit (New England Biolabs). A small sample was nanodropped and ran on a native denaturing gel to determine RNA concentration and verify full-length product ...
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bioRxiv - Synthetic Biology 2024Quote: ... 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), fully substituting UTP with N1-methylpseudouridine-5’-phosphate (TriLink Biotechnologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.2 μl 50x oligos of the AarI recognition site for Level 0 and Level 2 cloning or Eco31I/BsaI-HFv2 (ThermoFisher/NEB) for Level 1 cloning ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PstI-HF (NEB). The constructs were further verified by Sanger sequencing (GATC Biotech-Eurofins ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (ThermoFisher/NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reagents for cloning and PCR were purchased from NEB and Fisher Scientific.
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bioRxiv - Plant Biology 2024Quote: RNA for direct RNA sequencing was extracted from one white petal with the Monarch Total RNA miniprep Kit (New England BioLabs) combined with the manufacturer specific DNase treatment ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was treated with DnaseI (Rnase-free) (New England Biolabs) and 0.5-1 ug of RNA was used for cDNA synthesis using the Invitrogen cDNA synthesis kit SuperScript® RT ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 µl of T4 ligase (400 U/ µl, NEB), 1.5 µl of CutSmart Buffer (NEB) ...
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bioRxiv - Physiology 2024Quote: ... murine RNase inhibitor (500 U/mL, Cat. # M0314L; New England Biolabs, Frankfurt am Main, Germany) and heparin (10 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1μL of Taq DNA polymerase (NEB, M0267) were added for A-tailing of DNA ends ...
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... by XbaI (NEB; R0145L) and BamHI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Poly-A RNA was isolated from the total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs; E7490L or E7490S) and then quantified by a Qubit 3.0 Fluorometer (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: 50pmol of gRNA was combined with 25pmol of HiFi Cas9 Nuclease V3 (IDT, 1081060) in 1X CutSmart Buffer (NEB, B7204) at a final volume of 20μL ...
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bioRxiv - Molecular Biology 2023Quote: ... and BamHI (NEB; R3136L) restriction cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... which is then followed by a SalI (NEB; R0138L) restriction site ...
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... ligation reactions with T4 DNA ligase were prepared: 1 μM of ssPCRP was added to 1 μM complementary strands in 1X T4 ligation buffer (NEB) in a final volume of 10 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing adaptors from the Oxford Nanopore Ligation Sequencing Kit (ONT, LSK109) were ligated to DNA ends using Quick Ligase (NEB, M2200) for 10 minutes at room temp ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNAs were cloned into an all-in-one CRISPR/Cas9 vector using BbsI (NEB). The all-in-one CRISPR/Cas9 vector was cloned between AAV serotype 2 ITRs including a human U6 promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled total RNA was fragmented with ultrasound (4 pulses of each 30 sec at 4°C) and then treated with T4 Polynucleotide kinase (NEB). The RNA of each sample was then divided in half ...