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Citations for New England Biolabs :
7001 - 7050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2 µl 1 M DTT and 5 µl T4 polynucleotide kinase (NEB, 10 U/µl, #M0201) and incubated at 37 °C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... NEB10β competent cells (New England Biolabs, Ipswich, MA) were used for the amplification of pBOMB-derivative vectors ...
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bioRxiv - Microbiology 2024Quote: ... 1 μL of shrimp alkaline phosphatase (rSAP, NEB Catalog # M0371S), and 5 μg of fragmented DNA ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μL of dTTP (1 mM, NEB Catalog # N)443S) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed with Q5 Hot Start High-Fidelity Master Mix (NEB) and purified with Monarch PCR cleanup kit (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ExoI (New England Biolabs) treatment and 1.1×DNA FragSelect XP Magnetic Beads cleanup ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM Ribonucleoside Vanadyl Complex (NEB # S1402S - 200 mM), formamide (Midsci ...
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bioRxiv - Developmental Biology 2024Quote: Site-directed mutagenesis was performed on human HMGCR transcript 1 coding sequence in the pCMV-SPORT6 backbone to generate the variants of interest using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). pCMV-SPORT6-hHMGCR1 was a gift from Anne Galy (Addgene plasmid # 86085 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 20 U/mL DNase I (NEB). The mixture was triturated on ice for 20 seconds every 5 minutes until clumps of tissue were no longer visible ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or Phusion (New England Biolabs #M0530) or Q5 (New England Biolabs #M0491 ...
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bioRxiv - Genetics 2024Quote: ... and transformed into NEB Stable bacteria (New England Biolabs) to avoid recombination ...
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bioRxiv - Genetics 2024Quote: ... Each plasmid was assembled in a HiFi reaction (New England Biolabs) and transformed into NEB Stable bacteria (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Genomics 2024Quote: Adult worm Illumina sequencing libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Cat: E7805L) and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and digested by proteinase K (New England Biolabs, 1:100 dilution to 8 units ml−1) overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: These three PCR products were digested with XbaI and XhoI and cloned into XbaI- and XhoI-digested pMTL82151 plasmid using HiFi assembly (NEB). Electrocompetent E ...
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bioRxiv - Genomics 2024Quote: ... the Illumina flow cell adapters were added through a 15-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer pLSmP-ass-i741 and reverse primer pLSmP-ass-gfp ...
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bioRxiv - Genomics 2024Quote: ... The purified fragment underwent a second round of 12-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer 5BC-AG-f02 and the reverse primer 5BC-AG-r02 ...
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bioRxiv - Genomics 2024Quote: ... using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621L). The plasmid lentiMPRA library was electroporated into MegaX DH10B T1R Electrocomp Cells (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... A synthesized TWIST oligo pool with 300 bp long elements (270 bp insert + 2*15 bp PCR handles) was amplified by 12-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer 5BC-AG-f01 and reverse primer 5BC-AG-r01 were used to add the minimal promoter ...
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bioRxiv - Genomics 2024Quote: ... with modifications proposed by New England Biolabs (NEB) to ensure high yield data generation and long-fragment sequencing ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB, MA, United States, Cat N° E7180S), and AMPure XP beads (made in-house by the Molecular Biology Service ...
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bioRxiv - Genomics 2024Quote: ... 50ng of LentiCRISPR v2 (addgen, #52961) and 5ng of PCR-amplified sgRNA pool was mixed with 1ul of BsmB1-V2 restriction enzyme (NEB; #R0580) and 1ul of T7 ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... and 1ul of T7 ligase (NEB; M0202). Digestion and ligation of the vector to the oligos were conducted by 50 cycles of changing temperature (42.5-16°C ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries for good quality samples (total DNA amount > 20 ng) were prepared with the standard NEBNext®Ultra™ II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA), while double stranded libraries for the samples extracted in the ancient DNA laboratory were prepared following the Meyer and Kircher protocol67 ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol for 11 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Each gBlock was A-tailed using Taq DNA polymerase (New England Biolabs), ligated into pCR2.2-TOPO (TOPO ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The gRNA construct was generated by PCR with Phusion polymerase (NEB) and primers SOL897 and SOL898 (see Supplementary Table 4 for primer sequences ...
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5’ phosphorylated 3’ end adapter featuring 4 randomized nucleotides at the 5’ terminus and a 3’ blocking group (3C Spacer; 3SpC3, IDT) underwent adenylation using Mth RNA ligase (New England Biolabs, cat# M2611A). Adapters were subsequently ligated to deacylated and demethylated RNA templates using a truncated KQ T4 RNA ligase 2 (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... were ligated to the 3’ ends of total RNA from EVs and cellular samples isolated from an RN cell line using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 h at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: NEBNext 3’ SR adaptors for Illumina (New England Biolabs, cat# E7332) were ligated to the 3’ ends of total RNA from EVs and cellular samples isolated from an RN cell line using a truncated KQ T4 RNA ligase 2 (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 190 ng of linearized vector and 13.15 ng of each sub pool were used for each Gibson reaction of 80 µL using HIFI DNA Assembly Master Mix (NEB). The Gibson reaction was incubated for 1 hr at 50°C and DNA was isolated by isopropanol precipitation and transformed into Lucigen Endura Competent Cells according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently treated with Antarctic phosphatase (NEB) and the correct linearized backbone was isolated by gel electrophoresis and purified using Gel DNA Recovery Kit (Zymo) ...
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bioRxiv - Molecular Biology 2024Quote: ... We subjected 100 ng of DNA to a first round of PCR (25-28 cycles, Q5 hot start high-fidelity DNA polymerase (New England Biolabs)) to amplify the locus of interest and attach common overhangs ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (New England Biolabs). Recombinant expression for assessment of BcsF roles in macrocomplex assembly was carried out in a T1 phage-resistant 11bcs BL21*(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... NotI (-vRNA)) (NEB) and RNA was synthesized by in vitro transcription with T7 (+vRNA ...
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bioRxiv - Microbiology 2024Quote: ... The cloning method was followed according to a standard method from NEB 71 ...
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bioRxiv - Microbiology 2024Quote: ... and OneTaq (New England Biolabs) polymerase for inserts <5kb (PCR thermocycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... were amplified using the primers listed in Table S2 and cloned into the expression plasmids (van der Kolk et al., 2020) with NcoI and XhoI (New England Biolabs GmbH, Frankfurt am Main, Germany). Correct plasmids were methylated by transforming them to ER1821 containing pM.EsaBC4I ...
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bioRxiv - Microbiology 2024Quote: ... Sure2 or ER1821 (NEB) used for cloning and methylation of plasmid DNA respectively ...
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bioRxiv - Microbiology 2024Quote: ... expressing a methylase (NEB).
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bioRxiv - Microbiology 2024Quote: ... was performed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The ligation product was transformed into DH10B E.coli bacteria by heat shock ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of DNAse I at 1 mg/ml (NEB, cat no. M0303L), 1 μl RNAse at 10mg/ml (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl RNAse at 10mg/ml (NEB, cat no. T3018-1), and 1/10th volume of DNAse I reaction buffer (NEB cat ...
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bioRxiv - Microbiology 2024Quote: ... 10 μl of proteinase K at 20mg/ml (NEB cat no. P8107S) and a 2% final concentration of SDS was added and the mixture incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... a DNA library was generated using NEB Next Ultra II Q5 Master Mix (NEB# M0544L) and amplified by PCR as previously described (21) ...