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Citations for New England Biolabs :
7351 - 7400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and NEB5α (NEB, cat. #C2987) via heat shock as described above.
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bioRxiv - Microbiology 2023Quote: ... BL21 (NEB, cat. #C2530), and NEB5α (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Enzymatic deglycosylation was performed on denatured PrPres with 1,000 U of recombinant PNGase (peptide N-glycosidase F; New England Biolabs) for 2h at 37°C in 1% Nonidet P40 and the manufacturer’s buffer.
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bioRxiv - Microbiology 2023Quote: ... including Luna® Universal qPCR Master Mix (NEB, UK), one primer pair (cDNA template ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were performed using Q5 high-fidelity DNA polymerase (NEB, cat. #M0491S) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturer’s recommendations (New England Biolabs [NEB]; Ipswich, MA).
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bioRxiv - Microbiology 2023Quote: ... DNA fragments were generated using Q5 polymerase according to the manufacturer’s recommendation (New England Biolabs (NEB), USA) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments were generated using Q5 polymerase according to the manufacturer’s recommendation (New England Biolabs (NEB), USA) ...
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bioRxiv - Microbiology 2023Quote: ... using EcoRV (NEB) for linearization of the backbone plasmid ...
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bioRxiv - Microbiology 2023Quote: ... Phusion DNA-Polymerase (NEB) was used for the generation of PCR products for cloning ...
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bioRxiv - Microbiology 2023Quote: ... Phusion polymerase (NEB) was used to amplify the regions flanking the exsD gene ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were digested with the AvrII (CCTAGG) (NEB, Beverly, MA) restriction endonuclease ...
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bioRxiv - Microbiology 2023Quote: ... and ligated with T4 DNA ligase (NEB). The ligation reaction was loaded onto a 1% agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... 1x T4 DNA ligation buffer (NEB, B0202S), and 5U T4 PNK (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The spectinomycin cassette was digested with XbaI (TCTAGA) (NEB) from pUC19-Sprr loxP (76) ...
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bioRxiv - Microbiology 2023Quote: ... and was further prepared for sequencing using NEBNext Ultra FS II kit (NEB) with the miniaturized protocol (22) ...
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bioRxiv - Microbiology 2023Quote: ... and metagenomic libraries were prepared using 50 ng of DNA and the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) as per the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2023Quote: ... and HindIII-HF (NEB, R3104S) restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and 125U of Klenow fragment (NEB, M0212M). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... containing 1x rCutSmart buffer (NEB, B6004S), and 5U of quick CIP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA ends were filled and repaired using NEB Ultra II end-repair module (NEB #E7645), with adapters ligated sequentially to the sonicated ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplified yciF (with Strep II tag on C-terminal) and pQE60 plasmid (containing an IPTG inducible T5 promoter) were digested with BamHI and EcoRI (New England Biolabs) and purified by gel extraction (MinElute gel extraction kit ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed from the extracted RNA using DNase I (New England Biolabs) treatment and removal was confirmed by PCR of a reference gene ...
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bioRxiv - Microbiology 2023Quote: ... An additional DNase I (New England Biolabs, Ipswich, MA) treatment was performed at 37°C for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... 2X loading dye (NEB) was added the RNA and the sample was denatured at 70°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The inserts and backbone were assembled using the NEB HiFi DNA assembly kit (New England Biolabs, Ipswich, Massachusetts, USA), resulting in the final plasmids ...
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bioRxiv - Microbiology 2023Quote: ... Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB) to introduce point mutations for the T161V and D296N substitutions ...
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bioRxiv - Microbiology 2023Quote: ... and T4 polymerase (NEB); an ‘A’ base was added to the 3’ end of the blunt-end phosphorylated DNA fragments using the polymerase activity of Klenow (Exo-Minus ...
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bioRxiv - Microbiology 2023Quote: ... Obtained DNA was used for library preparation with NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB E7370L). Libraries were run on Novaseq 6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: DNA modification enzymes and PHUSION® polymerase for DNA amplification were all obtained from New England Biolabs (NEB) and were used as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... 220µL T4PNK (NEB), and 5µL of the reaction mix was distributed to each well of a 96-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5µL T7 DNA ligase (NEB), 1.2µL 50µM N7 oligo (5’-CAGCACGGCGAGACTNNNNNNNNNNGACTTGTC-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... 1µL 20mg/mL BSA (NEB). Importantly ...
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bioRxiv - Microbiology 2023Quote: ... The genomic DNA was digested with BstXI (New England Biolabs) for one hour at 37°C with enzyme inactivation at 65°C per manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The assembly was electroporated into 10B E.coli (NEB). The library construction of pUC57-NS5 was performed for each frame separately ...
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bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... were inserted into a pQE30 derivative plasmid pQE30_HGT (featuring an N-terminal His-tag connected to GFP via a TEV-cleavage site 41) using the Gibson isothermal method (NEBuilder HiFi DNA assembly master mix, New England BioLabs). Gene fragments were generated by PCR using primers (Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... and assembled via Gibson assembly using NEBuilder HiFi DNA Assembly MasterMix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and the desired product was agarose gel purified using a Monarch gel extraction kit (NEB) and sequenced on an Illumina MiSeq.
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Monophosphorylated RNA was synthesized from each of these PCR products in the presence of a 50-fold molar excess of AMP over ATP (74) with T7 RNA polymerase (NEB M0251). Each 50 μL reaction contained 1X reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... These were eluted and precipitated before ligation to 5’ adapters (1× T4 RNA ligase buffer (NEB), 0.5 µM p10IllLigup adapter ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA was treated with alkaline phosphatase (rSAP, NEB) instead of PNK to remove 5’ phosphorylation and prevent adapter concatenation during adapter ligation.
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bioRxiv - Molecular Biology 2023Quote: ... and T4 DNA ligase (NEB) using golden gate assembly ...
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bioRxiv - Molecular Biology 2023Quote: ... chilled on ice and then combined with 30 U T4 RNA Ligase 1 (NEB M0437M), 40 U Murine RNase Inhibitor (NEB) ...