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Citations for New England Biolabs :
7051 - 7100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Neuroscience 2024Quote: ... or NotI (NEB Cat # 189S) restriction endonuclease (depending on insert orientation) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ExoI (New England Biolabs) treatment and 1.1×DNA FragSelect XP Magnetic Beads cleanup ...
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bioRxiv - Microbiology 2024Quote: ... cloned into the pre-assembled vector using the NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2; NEB). A synonymous point mutation (A17973G ...
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bioRxiv - Microbiology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit from NEB was used according to the protocol and mutagenesis primers were designed using NEBaseChanger.
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bioRxiv - Microbiology 2024Quote: ... 2 µl 1 M DTT and 5 µl T4 polynucleotide kinase (NEB, 10 U/µl, #M0201) and incubated at 37 °C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... NEB10β competent cells (New England Biolabs, Ipswich, MA) were used for the amplification of pBOMB-derivative vectors ...
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bioRxiv - Microbiology 2024Quote: ... 1 μL of shrimp alkaline phosphatase (rSAP, NEB Catalog # M0371S), and 5 μg of fragmented DNA ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μL of dTTP (1 mM, NEB Catalog # N)443S) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Immunology 2024Quote: ... a DNA library was generated using NEB Next Ultra II Q5 Master Mix (NEB# M0544L) and amplified by PCR as previously described (21) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... the Luna Universal qPCR Master Mix Protocol (M3003 New England Biolabs) was used ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng of total RNA underwent ribosomal depletion with the NEBNext rRNA Depletion Kit (Bacteria) (NEB catalog # E7850) and library preparation with the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202 ...
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bioRxiv - Biophysics 2024Quote: ... were electro-transformed with PmeI-HF (New England Biolabs) linearized plasmids containing different mutant genes using a Gene Pulser II (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... and performed site-directed mutagenesis (E0554S, NEB) to generate Rb mutant plasmids ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were generated by Q5 Site-Directed Mutagenesis or Gibson Assembly (NEB). pDule2-pCNF was a gift from Ryan Mehl (Addgene plasmid # 85495 ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were prepared by NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, MA, USA) following manufacturer instructions and sequencing was conducted by NovaSeq 6000 (PE150 ...
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bioRxiv - Microbiology 2024Quote: ... The amplification product of pan-CoV semi-nested PCR was purified and concentrated by the Monarch PCR & DNA Cleanup Kit (NEB), according to the protocol supplied ...
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bioRxiv - Microbiology 2024Quote: ... digested using DpnI (New England Biolabs, #R0176S) per manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... Coli DH5⍺ (New England Biolabs, #C2987H) per manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and BamHI (NEB) sites.
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bioRxiv - Biochemistry 2024Quote: ... The intervening cDNA sequence was then amplified using LongAmp Taq polymerase (NEB). Primers for each gene region are shown in Supplementary Table S3 ...
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bioRxiv - Biochemistry 2024Quote: ... DNA templates were digested using RNase-free DNase (NEB), and RNA was phenol-chloroform extracted followed by isopropanol precipitation (overnight at −80°C) ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mix was incubated at 37°C and 15 ul aliquots were removed at indicated time points, quenched into stop buffer (1.8% SDS, 10 mM EDTA) followed by Proteinase-K (2 units total) (NEB) treatment for 45 min at 50°C ...
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bioRxiv - Biochemistry 2024Quote: In-vitro synthesized pri-miRNAs (100 pmol) were 5’-end labelled by T4-PNK (NEB) using [γ-32P]-ATP (Perkin Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were further treated with 2U Proteinase-K (NEB) for 45 min at 50°C before diluting the samples with 2X denaturing buffer (80 % formamide ...
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bioRxiv - Zoology 2024Quote: ... we generated a template for dsRNA synthesis by amplifying targeted coding sequences from cDNA using gene-specific primers with T7 overhangs and Q5 High-Fidelity DNA Polymerase (NEB, M0491S). Two rounds of PCR reactions (each with 35 cycles ...
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bioRxiv - Systems Biology 2024Quote: ... qPCR was performed using Luna qPCR master mix (NEB, M3004).
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bioRxiv - Systems Biology 2024Quote: ... and 2 μL of T4 PNK (10 U/μL, NEB) were added ...
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bioRxiv - Systems Biology 2024Quote: ... then 3 μL of USER enzyme (NEB) was added and mixed again by pipetting ...
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bioRxiv - Molecular Biology 2024Quote: ... The labeling of oligonucleotides at the 5’-end was carried out by T4 polynucleotide kinase (New England Biolabs) and [γ-32P] ATP (Hartmann Analytic) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.25 mg/ml BSA (New England Biolabs) and 1 nM oligonucleotide-based DNA substrate (in molecules) ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated with NotI-digested pCAG-Puromycin plasmid using T4 DNA ligase (NEB) to generate the pCAG-2XFLAG- BMAL1 plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragment was digested using NotI (NEB) and ligated with NotI-digested pCAG-Puromycin plasmid using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... during 8 PCR cycles with NEBNext HiFi PCR Master mix (New England Biolabs, #M0541S). Fragments of DNA 300 and 800 bp were then using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were indexed and amplified with NEBNext UDI primer pairs (New England Biolabs, #E6440S) during 8 PCR cycles with NEBNext HiFi PCR Master mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and A- tailed with the NEBNext dA-Tailing Module (New England Biolabs, #E6053L). After adapters ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... end-repaired with the NEBNext End Repair Module (New England Biolabs, #E6050L) and A- tailed with the NEBNext dA-Tailing Module (New England Biolabs ...
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bioRxiv - Systems Biology 2024Quote: ... 66U/μl truncated T4 ligase 2 and 13U/μl murine RNAse inhibitor (all from NEB, USA)) were added at 25°C for 1h ...
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bioRxiv - Systems Biology 2024Quote: ... and T4 DNA ligase (M0202L, New England Biolabs). The assembled plasmids were transformed into competent cells (C404003 ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids were cloned in house using Gibson Assembly with the NEB Hifi Builder Master Mix (New England Biolabs, Ipswich, MA) and/or contracted (GenScript ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmid E.coli plasmid DNA minipreps were performed using the NEB Monarch Plasmid Miniprep Kit and Protocol (NEB #T1010). Prior to starting the miniprep protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (High Efficiency) (#C3019H, NEB) or Electroporation Protocol (#C3020 ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Electroporation Protocol (#C3020, NEB). For electroporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... of PCR products carrying complementary USER junctions were mixed in a 10 μl reaction containing 0.75 units DpnI (New England Biolabs), 0.75 units USER enzyme (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning fragments were amplified with Phusion® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA) or Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Q5® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA). PCR products were digested with Dpn1 (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... sourced from NEB, and BL21(DE3) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with Dpn1 (NEB, Ipswich, MA, USA) to destroy the original template and were purified using the QIAquick Gel Extraction Kit (Qiagen ...