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Citations for New England Biolabs :
6851 - 6900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... using T4 DNA ligase (NEB). All PCRs were made using Q5 DNA polymerase (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... were ordered as gBlock gene fragments (Integrated DNA Technologies) and cloned into the NheI/EcoRI restriction sites of pLJM1 using Gibson Assembly (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... and heparinase III (NEB, P0737S) at 3 U/mL each ...
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were made using Q5 DNA polymerase (NEB). All constructs were sequence verified ...
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C single-index library preparation was performed as previously described67 using MboI (New England Biolabs) restriction enzyme.
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bioRxiv - Cancer Biology 2024Quote: ... DNA was purified and digested with the secondary RE (NlaIII, New England Biolabs) and circularized by ligation ...
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bioRxiv - Developmental Biology 2024Quote: ... and 20 U/mL DNase I (NEB). The mixture was triturated on ice for 20 seconds every 5 minutes until clumps of tissue were no longer visible ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L). PCR products were A-tailed ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.2 µL antarctic thermolabile UDG (New England BioLabs) and 250 nM each primer to a final volume of 10 µL ...
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bioRxiv - Developmental Biology 2024Quote: ... Each qPCR reaction mixture contained 5 µL 2x Luna Universal qPCR master mix (New England BioLabs), 1 µL cDNA (2-fold dilution) ...
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bioRxiv - Developmental Biology 2024Quote: ... The ssDNA overhangs were filled-in with T4 DNA polymerase (New England BioLabs) to generate dsDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 300 ng of purified RNA using LunaScript RT SuperMix kit (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplification of cDNAs was performed using a high-fidelity KOD-Plus-Neo DNA polymerase (Toyobo, Japan) and resulting PCR products were cloned using NEB® PCR Cloning kit (New England BioLabs). Positive clones and plasmids were verified by DNA sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the sgRNA was synthesized in vitro as described in the manual (HiScribe™ T7 High Yield RNA Synthesis Kit, NEB, NO. E2040S), the sgRNA and Cas9 protein were mixed and coinjected into the cell cytoplasm at the one-cell stage ...
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... IntBCs were inserted into the plasmids using the NEBuilder HiFi Assembly Master Mix (NEB). (3 ...
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bioRxiv - Developmental Biology 2024Quote: E.coli T7 Express strain (New Englad Biolabs) was transformed with p7XNH3-10xHis-3C-cCPE and pRare plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... and subjected to library construction using Q5 high-fidelity DNA polymerase (New England Biolabs, M0544S). Genomic DNA was fragmented using Tn5 transposase and subjected to library construction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 ng of genomic DNA and used the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) to prepare the libraries ...
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bioRxiv - Immunology 2024Quote: ... Pathogenic ADA2 variants were created by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (#E0554; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 units/mL RNase inhibitor murine (New England Biolabs)) ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Cell Biology 2024Quote: ... we used Sp6 RNA Polymerase (NEB) with dig-UTP as previously described (Toro-Tapia et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was utilized to construct the sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina as per the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... 2 μL of 10x DNase I Buffer (New England Biolabs, #m0303s), 1 μL of rDNase I (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mM dNTPs) and 1 µL of ProtoScript® II Reverse Transcriptase (NEB) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were joined using NEBuilder (New England Biolabs, Ipswich, MA, USA). The resulting construct was named pHY1399 ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissues (New England Biolabs, Ipswitch, MA, USA). Long-read sequencing was performed on the GridION platform (Oxford Nanopore Technologies ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Whole-cell extracts were quantified using the BCA assay and 10 µg was subjected to PNGase F (New England Biolabs, #P0705S) treatment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB). Library molarity was measured with the Qubit DNAds HS assay kit from Invitrogen and the quality was analyzed using Bioanalyzer DNA Analysis kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... This fragment was inserted by Gibson assembly (New England Biolabs) into Eps15-pmCherryN1 (Addgene plasmid#27696 ...
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bioRxiv - Immunology 2024Quote: ... coli (#C3040H, New England Biolabs) were transformed with the generated constructs ...
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bioRxiv - Microbiology 2024Quote: ... and 20 units RNase inhibitor (NEB) in 75 mM KCl ...
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bioRxiv - Plant Biology 2024Quote: ... The enriched products were purified and then subjected to enzymatic methylation conversion according to the manual of NEBNext Enzymatic Methyl-seq Conversion Module (NEB, Ipswich, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% sodium deoxycholate) with 1X protease inhibitor cocktail and 920 U of murine RNase inhibitor (M0314L, New England Biolabs, UK). Resuspended cell pellets were further lysed on ice with ten passes through an 18G blunt needle ...
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bioRxiv - Microbiology 2024Quote: ... The L gene was assembled into a pIRES vector digested NheI and XbaI using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) according to manufacturer instructions to generate pCMV-Lopt ...
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bioRxiv - Microbiology 2024Quote: ... Foreign glycoproteins were then cloned into this vector by either digestion with MluI and PacI or by seamless cloning using the HiFi NEBuilder (NEB) kit ...
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bioRxiv - Cell Biology 2024Quote: ... Truncated versions of FLAG::TurboID::SQSTM1 were generated by PCR using primers corresponding to the truncated SQSTM1 gene and amplification using Q5 high-fidelity (M0492, NEB). Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S ...
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bioRxiv - Biophysics 2024Quote: ... Strand specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), mRNA was isolated using Poly(A ...
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bioRxiv - Biophysics 2024Quote: ... were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318 ...
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bioRxiv - Neuroscience 2024Quote: ... GDNA was isolated from 7 dpf efemp1+/+ or efemp12C-Cas9 zebrafish eyes using a Monarch Genomic DNA Purification Kit (T3010S; NEB) and quantified using a Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and BsmBI-v2 enzymes (R0739S; NEB) targeting the restriction sites behind the U6 promoters to remove the pre-set sequences that are to be replaced by DNA sequences of small guide RNA (sgRNA) ...
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bioRxiv - Neuroscience 2024Quote: ... was digested using BsaI (R0535S; NEB, Ipswich, MA, USA) and BsmBI-v2 enzymes (R0739S ...
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bioRxiv - Genetics 2024Quote: ... Phusion High Fidelity Master Mix with GC Buffer (NEB, Massachusetts, United States) was used ...
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bioRxiv - Bioengineering 2024Quote: ... The transgene and sex of the F0 and F1 pups were determined by 3 PCRs of the Y chromosome using oligonucleotides described in Table S2 and LongAmp DNA polymerase (New England Biolabs, USA). Thermocycling conditions were as follows ...
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bioRxiv - Plant Biology 2024Quote: ... and thermostable FEN1 (New England Biolabs, Ipswich, USA). Ligation was initiated by adding a stock mixture of oligos-3 for all 108 targets ...
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bioRxiv - Plant Biology 2024Quote: DNA not ligated to U2 oligo and therefore not protected by 3’ C3 Spacer was removed by addition of exonucleases I and III (NEB, Ipswich, USA). The enriched products were purified and then subjected to enzymatic methylation conversion according to the manual of NEBNext Enzymatic Methyl-seq Conversion Module (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Electroligase kit (NEB). Electrocompetent ElectroMax Stbl4 bacteria (Invitrogen ...