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Citations for New England Biolabs :
6801 - 6850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Additional mutations/deletions were introduced in gp140-T/F07 using site directed mutagenesis kit (NEB). The sequences of the recombinant clones were verified through sequencing (Retrogen ...
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bioRxiv - Genomics 2024Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 1 hour and later transferred to 100 mL of LB medium with ampicillin overnight ...
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bioRxiv - Genomics 2024Quote: ... The backbones for the Gibson reaction for GRB2-SH3 and PSD95-PDZ3 library assembly (aPCA plasmids) were first linearized using primers listed in Extended Data Table 3 and next treated with Dpn1 (NEB) restriction enzyme to remove the circular plasmid template ...
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bioRxiv - Genomics 2024Quote: ... the pGJJ162 backbone was linearised by digestion with restriction enzymes NheI and HindIII (NEB) for the Gibson reaction ...
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bioRxiv - Genomics 2024Quote: ... and amplified using ISOSDB412 IS-Seq Step1 and p7 primers for 13 cycles using Q5 Master Mix (New England Biolabs, Ipswich, MA). The products of this reaction were amplified with IS-Seq Step2 and p7 primers for 9 cycles using Q5 Master Mix and sequenced on a Novaseq 6000 at Novogene (Sacramento ...
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bioRxiv - Genomics 2024Quote: ... 20 U of T4 Polynucleotide kinase (NEB), 3.5 U of Large (Klenow ...
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bioRxiv - Genomics 2024Quote: ... All plasmid constructs were made using Gibson assembly master mix (New England Biolabs, Ipswich, MA). Assembled constructs were electroporated into electrocompetent E ...
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bioRxiv - Microbiology 2024Quote: ... The gels were loaded with a known amount of 100bp DNA ladder (NEB) alongside samples of each reaction to quantify their concentrations based on band intensity around 250-600bp using a gel imaging system ...
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bioRxiv - Microbiology 2024Quote: ... and 50% PEG8000 (NEB) to allow for 3’ linker ligation at room temperature for 1.5 hours ...
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bioRxiv - Microbiology 2024Quote: ... FastAP (Thermo Scientific) (and murine RNase inhibitor; NEB) for 30min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Gibson assembly was performed following the manufacturer’s instruction (NEB, E2621S) and transformed into chemically competent DH10B cells ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 mM G(5’)ppp(5’)G RNA Cap Analogue (New England Biolabs), 4 μg DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 62.5 units per 1 million cells MNase (NEB; M0247S) was added to resuspended pellets ...
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bioRxiv - Physiology 2024Quote: ... the pLV6 backbone and the Per2:luciferase fragment were ligated with T4 DNA ligase (NEB, M0202S) according to the manufacture’s manual ...
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bioRxiv - Cell Biology 2024Quote: ... by linearizing the vector with 15 base pairs (bp) overlapped primers using Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. MO531L). PCR products were digested using Dpn I (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... and assembled into a digested (HindIII, rCutSmart®, New England Biolabs, catalog #R0104) and purified pAG416-GPD-ccdB using in-house Gibson assembly ...
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bioRxiv - Biochemistry 2024Quote: ... truncated KQ (NEB, #M0373S) and T4 RNA Ligase 1 (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... pAG416-GPD-ccdB was digested using XbaI and HindIII-HF in CutSmart® buffer (New England Biolabs, catalog #R0145 and #R0104), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... A 673 bp digoxigenin-labeled DNA handle was amplified in Q5U PCR master mix (NEB, Cat# M0597L) with a forward primer containing a deoxyuridine and a reverse primer with a 5′ digoxigenin tag ...
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bioRxiv - Neuroscience 2024Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS, 2% BSA, 0.2U/µl RNase inhibitor; New England Biolabs, cat# M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their morphology ...
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bioRxiv - Neuroscience 2024Quote: ... All lentiviral constructs were generated through the Gibson assembly method (NEB) with the corresponding cDNAs and with a human synapsin-1 promoter-driven lentiviral shuttle vector (f(syn) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was digested with Ncol-HF (New England BioLabs, Notting Hill, Australia). Real-time PCR reactions were performed using RT2 Forget-Me-NotTM EvaGreen qPCR Mastermix (Biotium ...
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bioRxiv - Genetics 2024Quote: ... 5ug of genomic DNA was dephosphorylated in a 30ul reaction volume using 15U of Quick calf intestinal phosphatase (NEB cat #M0525) and 3ul of 10x rCutsmart Buffer (NEB B6004S) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Evolutionary Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix and T4 DNA ligase from New England Biolabs (NEB). We obtained oligonucleotides from Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 7.5 ng of plasmid backbone and 2.5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB). We incubated the reaction mix for 16 h at 50 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from each homogenized pool of 100 flies using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA quality was assessed using Nanodrop and quantified with Qubit ...
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bioRxiv - Genomics 2024Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 0.25 μM of adapter and 2 μL of Quick ligase (NEB) for 20 minutes at 23°C in 40 μL ...
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bioRxiv - Genomics 2024Quote: ... 0.2 mM of dATPs and 7.5 U of Klenow fragment-exo (NEB) at 37°C for 60 minutes in 25 μL ...
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bioRxiv - Genomics 2024Quote: ... Then 3 µL USER Enzyme (NEB, Ipswich, MA, USA) was used with size-selected ...
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bioRxiv - Genomics 2024Quote: ... 0.5 µM GAT-7N random hexamer primers with an adapter sequence and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L). DNA was further amplified as in the pre-amplification step above ...
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bioRxiv - Genomics 2024Quote: ... followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®). Standard experimental conditions ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...
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bioRxiv - Genomics 2024Quote: ... NEB buffer 3.1 (NEB) and 270 nM of HBG-sgRNA (Synthego) ...
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bioRxiv - Immunology 2024Quote: ... recombinant human Furin (New England Biolabs Inc., Ipswich, MA, USA) was diluted in PBS and added to assays as indicated at a concentration of 10 U/ml ...
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bioRxiv - Microbiology 2024Quote: ... restriction enzymes and T7 ligase (NEB Biolabs, USA) were used according to manual ...
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bioRxiv - Immunology 2024Quote: ... vector were digested using AscI and SbfI (New England Biolabs) and ligated by T4 DNA ligase (Promega Corporation) ...
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bioRxiv - Immunology 2024Quote: ... transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (#E2040S, NEB) and purified with RNA Clean & Concentrator™ (#R1017 ...
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bioRxiv - Genomics 2024Quote: ... Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Genomics 2024Quote: Adult worm Illumina sequencing libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Cat: E7805L) and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and digested by proteinase K (New England Biolabs, 1:100 dilution to 8 units ml−1) overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were amplified with Q5 Polymerase (New England Biolabs) with an initial denaturation at 98°C ...
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bioRxiv - Plant Biology 2024Quote: ... construct-specific endonucleases and T4 DNA ligase (all enzymes from NEB BioLabs), according to the manufacturer’s directions ...
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bioRxiv - Plant Biology 2024Quote: ... and truncated Illumina adapters were added with T4 DNA ligase (NEB) overnight at 16 degrees C ...
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bioRxiv - Plant Biology 2024Quote: ... construct-specific endonucleases and T4 DNA ligase (all enzymes from NEB BioLabs), according to the manufacturer’s directions ...
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bioRxiv - Synthetic Biology 2024Quote: ... compatible overhangs for ligation were generated by digestion of PCR products using 5 units each of BsaI (NEB, R3733S) and DpnI (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... and DpnI (NEB, R0176S) in 60 uL of 1x CutSmart buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... were cloned using NEBuilder® HiFi DNA Assembly (NEB, Catalog #E2621X). ITRs were mutated to produce scAAV56 ...
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bioRxiv - Synthetic Biology 2024Quote: ... All fragments were digested with DpnI (NEB) overnight at 37°C in the PCR buffer and PCR purified using the NEB Monarch® PCR & DNA Cleanup Kit the next day ...