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Citations for New England Biolabs :
451 - 500 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... We digested 10 µg of RCP83 using 10 µL SfiI enzyme (NEB, #R0123S) in 50 µL 10x NEB CutSmart buffer and water to 500 µL ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µg cell lysate was treated with 80 units of lambda-phosphatase (NEB) at 30°C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 µg of purified DNA was fragmented using dsDNA Fragmentase (New England Biolabs). Then ...
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bioRxiv - Biophysics 2021Quote: ... 0.1 mg/mL BSA and 6.4 µg of Lambda DNA (New England Biolabs). The excess lambda DNA was added to the to eliminate non-specific sticking of histones to linker DNA and prevent any aggregation that can occur in an excess of histone proteins ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL cycloheximide) supplemented with 0.1 U/µL murine RNase Inhibitor (NEB), and passed through a G25 needle 25 times ...
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bioRxiv - Plant Biology 2023Quote: ... treated with 5 µg/mL Proteinase K (New England Biolabs Inc., MA, US) for 5 mins ...
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bioRxiv - Microbiology 2023Quote: 1 µg of total RNA was treated with DNase-I (New England Biolabs) followed by cDNA synthesis using iScript cDNA synthesis kit (Bio-rad ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
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bioRxiv - Immunology 2024Quote: One µg of SQBs was incubated with 1.0 U/µL DNase I (NEB) with 10 × DNase I buffer diluted in water (New England Biolabs #M0303S) ...
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bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
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bioRxiv - Molecular Biology 2023Quote: ... Double-stranded RNA was purified using Monarch RNA Cleanup Kit (50 µg; NEB) and eluted in 30 μl of nuclease-free water ...
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bioRxiv - Genomics 2024Quote: ... 2 µg of the sgRNA-barcode library was linearized with AscI (NEB, R0558S) and ...
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bioRxiv - Genomics 2024Quote: ... was utilized using 1 µg gDNA and the pre-annealed NEB adapter (NEB_P7 ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin/streptomycin (100U/mL penicillin and 100 µg/mL streptomycin, Nordic Biolabs), and supplemented with 1mg/mL geneticin (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 µg of DNA was hydrolyzed with a nucleoside digestion mix (NEB). The resulting nucleoside solution was analyzed by HPLC and mass spectrometry (LC/MS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of purified RNA was immobilized on streptavidin magnetic beads (NEB) for 8 h at 4°C under orbital rotation in binding buffer (24 mM KCl ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg of isolated RNA was treated with DNase I (NEB, Cat: M0303S) to remove any contaminating genomic DNA ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA was purified using the Monarch RNA Cleanup Kit (500 µg, NEB). The concentration of the RNA was determined by measuring the absorbance at 260 nm using a NanoDrop ...
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bioRxiv - Genomics 2024Quote: ... Dam methyltransferase (NEB M0222B-HC2 at 40 U/µl; 8 µg Dam/ml) was added to the aliquots of nuclei ...
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bioRxiv - Synthetic Biology 2020Quote: ... Both linear DNA fragments were finally used in a standard restriction digest using EcoRI (New England Biolabs, R3101) and NotI (New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... was achieved by PCR of the relevant design using primers F_RiboJ_Rem and R_RiboJ_Rem (Supplementary Table 5) and subsequent circularization by standard Golden Gate assembly (New England Biolabs, E1601S) to create the plasmids pVB003 and pVB004 ...
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bioRxiv - Biochemistry 2020Quote: ... PCR was carried out under standard conditions using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). Samples were kept at 98 °C for 30 s (1 cycle) ...
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bioRxiv - Cell Biology 2022Quote: ... Qualifying samples were prepped following the standard protocol for the NEBNext Ultra II Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads ...
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bioRxiv - Synthetic Biology 2021Quote: ... 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μl Chai Green Dye 20X (Catalog No ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Genomics 2020Quote: ... 1993) and transformation efficiencies were routinely evaluated using a standard batch of the commercial supercoiled plasmid pLITMUS38 (NEB). All E.coli strains used were obtained from New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... Semi-quantitative PCR was performed using Taq DNA polymerase with standard Taq buffer (New England BioLabs Cat # M0273S). PCR products were analyzed on 2% Agarose gels with 0.5 ng/L Ethidium bromide using a 1kb Plus DNA Ladder (New England BioLabs Cat # N3200S ...
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bioRxiv - Synthetic Biology 2023Quote: ... 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.),1.25 μl Chai Green Dye 20X (Catalog No ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then prepped according to the standard protocol for NEBnext Ultra ii Stranded mRNA (New Englad Biolabs). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Synthetic Biology 2023Quote: ... All plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template in 25 µl of 1x One Taq Standard Buffer (Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... 10µM of each primer and 10 μl of OneTaq 2X Master Mix with Standard Buffer (New England Biolabs) was used for double-strand DNA synthesis ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... Either 3-5 µL of Color Prestained Protein Standard-Broad Range (11-245 kDa) (New England Biolabs, P7712) or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa ...
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bioRxiv - Evolutionary Biology 2023Quote: Qualifying samples were prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs) at Biomedical research Center sequencing Core at University of British Columbia ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted DNA libraries were quantified by qPCR using NEBNext® Library Quant DNA Standards (New England Biolabs, E7642S) and iTaq™ Universal SYBR® Green Supermix as described (Ferguson et al ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic region containing the CRISPR-edit was PCR-amplified using OneTaq polymerases in Standard Buffer (New England Biolabs). After PCR ...
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bioRxiv - Physiology 2024Quote: ... PCR reactions were performed using the Taq DNA Polymerase with Standard Taq Buffer (New England Biolabs, Ipswich, MA) on a T100 Thermal Cycler (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... The qPCR reactions were prepared following the standard protocol for the Luna kit (New England Biolabs, Ipswich, MA). Oligonucleotide sequences are in Table S2.
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bioRxiv - Biophysics 2024Quote: ... Template digestion and PCR product ligation were completed using the KLD Enzyme Mix standard protocol (New England Biolabs). The PCR products were transformed into DH5α competent cells and incubated overnight on LB-agar plates containing 100μM ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... with 50 ng of digested backbone and 10-40ng of gel purified cDNA following standard protocol from NEB. Assembled constructs were transformed into NEB DH5alpha (NEB Cat ...
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bioRxiv - Molecular Biology 2021Quote: End-repaired nucleosomes were then subjected to proximity ligation with ∼5000 cohesive end units (CEU) of T4 DNA ligase (NEB #M0202) in 1X T4 DNA ligase reaction buffer (NEB #B0202 ...
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bioRxiv - Genomics 2020Quote: Biotinylated-nucleosomes were then subjected to the proximity ligation reaction with 5000 cohesive end units (CEU) of T4 DNA ligase (NEB #M0202) in 1X T4 DNA ligase reaction buffer (NEB #B0202 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µL of a 1000 U of DNase I stock was added to 500 µL of DNase I buffer on cells for 10 minutes or 2 µL of a 5000 U of RNase H stock (NEB M0297S) was added to 500 µL of RNase H buffer on cells for 5 minutes at 45 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... we used 50 μL Protein A or Protein G Magnetic beads (NEB) and washed twice with PBS with 5 mg/ml BSA and 4 μg of antibody coupled in 500 μl PBS with 5 mg/ml BSA overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... A VSV-G plasmid (16) was digested with KpnI and HpaI (NEB), removing a C-terminal part of G ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: Library preparation from co-immunoprecipitated RNA samples were carried out with NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs) that allow sequencing in Illumina platforms ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg Cas9 plasmid targeting the TgIST C-terminus was co-transfected with 40ug of the TgIST-GeneSwap-DiCre vector linearized with ScaI restriction enzyme (NEB). Drug selection with DMEM complete media containing 25µg/mL mycophenolic acid and 50µg/mL xanthine was performed 24hrs post-transfection for the following 6 days before removing selection media and subcloning by limiting dilution after sufficient parasite egress was observed.
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bioRxiv - Biochemistry 2020Quote: ... SMN-Gemin2 complex was produced by co-expression of SMN (residues 1-294) and Gemin2 (12-280) fused to a C-terminal Mxe intein (NEB) containing a hexahistidine tag ...