Labshake search
Citations for New England Biolabs :
6751 - 6800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Pathogenic ADA2 variants were created by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (#E0554; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 units/mL RNase inhibitor murine (New England Biolabs)) ...
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bioRxiv - Cell Biology 2024Quote: ... This fragment was inserted by Gibson assembly (New England Biolabs) into Eps15-pmCherryN1 (Addgene plasmid#27696 ...
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bioRxiv - Cell Biology 2024Quote: ... we used Sp6 RNA Polymerase (NEB) with dig-UTP as previously described (Toro-Tapia et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was utilized to construct the sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina as per the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... 2 μL of 10x DNase I Buffer (New England Biolabs, #m0303s), 1 μL of rDNase I (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mM dNTPs) and 1 µL of ProtoScript® II Reverse Transcriptase (NEB) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Immunology 2024Quote: ... coli (#C3040H, New England Biolabs) were transformed with the generated constructs ...
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bioRxiv - Microbiology 2024Quote: ... and 20 units RNase inhibitor (NEB) in 75 mM KCl ...
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bioRxiv - Plant Biology 2024Quote: ... The enriched products were purified and then subjected to enzymatic methylation conversion according to the manual of NEBNext Enzymatic Methyl-seq Conversion Module (NEB, Ipswich, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% sodium deoxycholate) with 1X protease inhibitor cocktail and 920 U of murine RNase inhibitor (M0314L, New England Biolabs, UK). Resuspended cell pellets were further lysed on ice with ten passes through an 18G blunt needle ...
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bioRxiv - Biophysics 2024Quote: ... Strand specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), mRNA was isolated using Poly(A ...
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bioRxiv - Biophysics 2024Quote: ... were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318 ...
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bioRxiv - Neuroscience 2024Quote: ... GDNA was isolated from 7 dpf efemp1+/+ or efemp12C-Cas9 zebrafish eyes using a Monarch Genomic DNA Purification Kit (T3010S; NEB) and quantified using a Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and BsmBI-v2 enzymes (R0739S; NEB) targeting the restriction sites behind the U6 promoters to remove the pre-set sequences that are to be replaced by DNA sequences of small guide RNA (sgRNA) ...
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bioRxiv - Neuroscience 2024Quote: ... was digested using BsaI (R0535S; NEB, Ipswich, MA, USA) and BsmBI-v2 enzymes (R0739S ...
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bioRxiv - Genetics 2024Quote: ... Phusion High Fidelity Master Mix with GC Buffer (NEB, Massachusetts, United States) was used ...
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bioRxiv - Bioengineering 2024Quote: ... The transgene and sex of the F0 and F1 pups were determined by 3 PCRs of the Y chromosome using oligonucleotides described in Table S2 and LongAmp DNA polymerase (New England Biolabs, USA). Thermocycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... The L gene was assembled into a pIRES vector digested NheI and XbaI using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) according to manufacturer instructions to generate pCMV-Lopt ...
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bioRxiv - Microbiology 2024Quote: ... Foreign glycoproteins were then cloned into this vector by either digestion with MluI and PacI or by seamless cloning using the HiFi NEBuilder (NEB) kit ...
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bioRxiv - Cell Biology 2024Quote: ... Truncated versions of FLAG::TurboID::SQSTM1 were generated by PCR using primers corresponding to the truncated SQSTM1 gene and amplification using Q5 high-fidelity (M0492, NEB). Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Microbiology 2024Quote: ... The resulting upstream and downstream amplicons were fused by PCR with the primers listed in Table S1 and the fused PCR product was purified and digested with the HindIII restriction enzyme (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2024Quote: ... purified using column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with Nhe1 and BamH1 double-digested and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 ...
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bioRxiv - Molecular Biology 2024Quote: ... then the STA products were treated with Exonuclease I (New England Biolabs, Ipswich, MA, USA) before 20-fold dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB). tRNA library preparation used SuperScript™ IV following the QuantM-tRNA-seq protocol (Pinkard et al ...
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bioRxiv - Systems Biology 2024Quote: ... and 0.5 µl DpnI (New England Biolabs) for 1 hr at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Restriction enzyme HindIII (New England Biolabs), was used in the suggested concentration to digest DNA prior to ddPCR.
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bioRxiv - Systems Biology 2024Quote: ... 0.5 µl of Q5 DNA polymerase (New England Biolabs) and molecular biology grade water to a total volume of 45 µl ...
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bioRxiv - Plant Biology 2024Quote: ... and thermostable FEN1 (New England Biolabs, Ipswich, USA). Ligation was initiated by adding a stock mixture of oligos-3 for all 108 targets ...
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bioRxiv - Plant Biology 2024Quote: DNA not ligated to U2 oligo and therefore not protected by 3’ C3 Spacer was removed by addition of exonucleases I and III (NEB, Ipswich, USA). The enriched products were purified and then subjected to enzymatic methylation conversion according to the manual of NEBNext Enzymatic Methyl-seq Conversion Module (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Electroligase kit (NEB). Electrocompetent ElectroMax Stbl4 bacteria (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR was performed using LUNA SYBR green reagent (New England Biolabs) on a Qiagen Rotorgene instrument ...
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bioRxiv - Genomics 2024Quote: ... 1 µg of genomic DNA was pooled with 1:20 dilutions of unmethylated lambda (1 µl of 0.1 ng/µl) and methylated pUC19 control DNA (1 ul of 0.005 ng/µl) from the EM-seq kit (E7120L; NEB, Ipswich, MA). Volumes were made up to 50 ul with 0.1x TE buffer (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... 200 ng of sheared DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (E7120; NEB, Ipswich, MA) following the manufacturer’s instructions for large insert libraries ...
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bioRxiv - Immunology 2024Quote: The 200 ng extracted mRNA was digested into nucleosides by Nuclease P1 (1 U, NEB, M0660S) and shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Immunology 2024Quote: ... and shrimp alkaline phosphatase (rSAP, 1 U, NEB, M0371S) in 50 μL RNase-free water at 37 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
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bioRxiv - Cell Biology 2024Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Cell Biology 2024Quote: ... For the detection of 1 kb DNA ladder (NEB, #N3232) and 𝜆 HindIII/𝜑X HaeIII marker ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Microbiology 2024Quote: ... All modifications were carried out by Gibson assembly (NEB, Ipswich, MA, USA), using PCR products from other plasmids or synthesized DNA fragments (GeneArtTM StringTM fragments ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was used as a template for PCR (Phusion HF kit (New England Biolabs)) with primers designed to amplify the spike and HE genes (Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) cells (New England Biolabs Frankfurt, Germany). Starter cultures (5 mL ...
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bioRxiv - Genomics 2024Quote: ... Excessive primers were digested by Thermolabile Exonuclease I (NEB, M0568) at 37 °C for 30 min ...
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bioRxiv - Genomics 2024Quote: ... End repair was performed in T4 ligase reaction buffer (New England Biolabs, NEB), 0.4mM of dNTPs ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was digested with exonuclease V (New England Biolabs) overnight at 37 °C to remove as much as possible linear dsDNA fragments and keep the circular DNA intact ...
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bioRxiv - Evolutionary Biology 2024Quote: ... treated with Dpn1 (New England Biolabs) at 37 °C for 45 min ...