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Citations for New England Biolabs :
6701 - 6750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Mutants were re-transformed into BL21(DE3) (NEB) cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutants were generated through inverse PCR (iPCR) on the pET23-KOD-Exo-(Table S2) plasmid in Q5 High-Fidelity DNA Polymerase (NEB) reactions of 30 cycles following the manufacturer’s standard protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning was performed using restriction enzymes and HiFi DNA assembly (NEB). Primers (Eurofins Genomics ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 µl of DNase I (2500U/mL, NEB) for each mL of B-PER reagent used ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of ExoI and 1 µl rSAP (NEB) were added to the recovery products and incubated for 15 min at 37°C followed by heat inactivation of 20 min at 80°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli strains NEB Stable and NEB 10β (NEB) were cultured in Luria–Bertani (LB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells (NEB). The cells were freshly prepared to maximise transformation efficiency ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid assembly was performed using the HiFi DNA Assembly (New England Biolabs) according to the supplier’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: All DNA constructs and oligos were designed using either Snapgene (BioMatters Ltd) or Primer3 (Koressaar and Remm 2007) and plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Cancer Biology 2024Quote: ... indel assay was performed by T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) to detect how efficiently a given sgRNA produces indels at targeted genomic DNA locus ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext Ultra II Q5 Master Mix (New England BioLabs) incubated at 98 °C for 2 min followed by 7 cycles of 98 °C for 20 sec ...
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bioRxiv - Cancer Biology 2024Quote: ... and KpnI (NEB, #R3142S) restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: ... and libraries were prepared using NEBNext Ultra II DNA Library Kit from Illumina (New England Biolabs, cat. no. E7645S).
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was submitted for small RNA sequencing to the UT Southwestern McDermott NGS Core using the NEBNext Small RNA kit (New England Biolabs, cat. no. E7330), with size selection between 15bp and 30bp ...
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bioRxiv - Physiology 2024Quote: ... Strand-specific libraries were prepared by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB, USA), as described previously (Makhnovskii et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... phosphorylated using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA), and ligated into digested BsmBI-digested (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 600 U of HindIII (New England Biolabs) was added ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissues (New England Biolabs, Ipswitch, MA, USA). Long-read sequencing was performed on the GridION platform (Oxford Nanopore Technologies ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Whole-cell extracts were quantified using the BCA assay and 10 µg was subjected to PNGase F (New England Biolabs, #P0705S) treatment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB). Library molarity was measured with the Qubit DNAds HS assay kit from Invitrogen and the quality was analyzed using Bioanalyzer DNA Analysis kit (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 ng of genomic DNA and used the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) to prepare the libraries ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteinase K (300 µg; New England Biolabs) was added to ligation mixtures and non-ligated controls ...
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bioRxiv - Microbiology 2024Quote: ... or Q5 High-Fidelity 2X Master Mix (NEB), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and adenylated using a 5′ DNA adenylation kit at the 5′ end (NEB). To reduce ligation bias ...
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bioRxiv - Genomics 2024Quote: ... and 0.5 µL of Murine RNase Inhibitor (NEB M0314S) were incubated for 30 minutes at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of 10 mM ATP (NEB P0756S), 1 µL of E ...
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bioRxiv - Genomics 2024Quote: ... coli Poly(A) Polymerase (NEB M0276S) and 0.5 µL of Murine RNase Inhibitor (NEB M0314S ...
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bioRxiv - Genomics 2024Quote: ... 8 µL of 5X Induro RT Buffer (NEB M0681S), 12.6 µL nuclease free water ...
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bioRxiv - Genomics 2024Quote: ... 0.4 µL RNase Inhibitor and 2 µL Induro RT (200 U/µL; NEB M0681S) were incubated for 15 minutes at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 4-hour linearization with XbaI endonuclease (NEB, Canada) at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... All restriction digest reactions were performed with enzymes provided by NEB according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... coli NEBα (NEB, Canada) competent cells and further recovered using GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were joined using NEBuilder (New England Biolabs, Ipswich, MA, USA). The resulting construct was named pHY1399 ...
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bioRxiv - Molecular Biology 2024Quote: ... was confirmed using a Lambda phosphatase assay (New England BioLabs).
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bioRxiv - Molecular Biology 2024Quote: ... and 11µl Murine RNase Inhibitor (New England BioLabs Inc® M0314L) for 15 mins and were then centrifuged at 20,000 g for 20 mins at 4°C ...
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bioRxiv - Neuroscience 2024Quote: We amplified the full-length Ncan sequence by PCR from the mouse brain cDNA library and assembled it into the pCAG-GFP vector using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) or In-Fusion HD Cloning Kit (TaKaRa) ...
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bioRxiv - Microbiology 2024Quote: ... All PCR-generated probes were treated with exonuclease I (NEB) according to the manufacturer’s protocol to remove single-stranded DNA ...
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bioRxiv - Microbiology 2024Quote: ... the NEBNext Ultra II DNA Library Prep Kit (NEB) was used according to the manufactu er’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... illumina sequencing libraries were prepared with a NEBNext®Ultra™DNA Library preparation kit (New England Biolabs, USA) of 500 mg DNA samples from each sample ...
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bioRxiv - Plant Biology 2024Quote: ... dNTPs and hot start Taq polymerase (New England Biolabs Inc, Ipswich, MA). A construct specific primer (Forward ...
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bioRxiv - Plant Biology 2024Quote: ... then pooled and ONT adapters were added using the NEBNext Quick Ligation Module (NEB). After purification with AMPure XP beads (Beckmann Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... Genes of interest were cloned to Gateway™ pENTR™ 4 Dual Selection Vector (A10465) by NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) and cloned to a pUC19 expression vector powered by the maize ubiquitin promoter (ZmUBI ...
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bioRxiv - Plant Biology 2024Quote: ... Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB). Barcoded fragments were purified with AMPure XP beads (Beckmann Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... The Q5 NEB Mutagenesis Kit (New England Biolabs) was used to add an NES (LQKKLEELEL ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was incubated for 3 days at 37C in the dark for conjugation and purified for 3 rounds using Monarch PCR & DNA Cleanup Kit (5 µg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Transposon insertion sites were then amplified using Q5 hot start polymerase (NEB) and 0.6µM Nextera 2A-R and olj638 (for Himar-1 libraries) ...