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Citations for New England Biolabs :
6601 - 6650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... with T4 Ligation Buffer (NEB). Duplexed oligonucleotides were ligated into dephosphorylated and BsmBI-digested pLB-Cas9 using the Quick Ligase kit (NEB M2200S ...
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bioRxiv - Genomics 2024Quote: ... Small RNA libraries were made using NEBNext Small RNA Library Prep Set (NEB #E7330) with up to 5µg of RNA input ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of the WTA library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Purified adapter-ligated products were subjected to index PCR ...
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bioRxiv - Plant Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... and 8 U RNase Inhibitor Murine (New England Biolabs). The reaction was incubated at 50°C for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA was amplified using Q5 Polymerase (New England Biolabs) with an initial denaturation at 98°C for 30 seconds ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting cDNA and qDNA were amplified with Q5 Polymerase (New England Biolabs), and the gel-purified PCR products were sequenced.
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bioRxiv - Plant Biology 2024Quote: ... DNase I-treated total RNA from Arabidopsis was used for reverse transcription with Induro (New England Biolabs) and random hexamers ...
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bioRxiv - Plant Biology 2024Quote: Reverse transcription was performed with Induro (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting amplicons were cloned into the pMiniT2.0 vector (New England Biolabs), and plasmid DNA from 18 colonies was purified and Sanger sequenced ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µg of total RNA was circularised in a reaction containing 6 U T4 RNA Ligase 1 (New England Biolabs), 50 µM ATP ...
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bioRxiv - Plant Biology 2024Quote: ... A-tailed with Klenow 3’-5’ exo-(NEB), and truncated Illumina adapters were added with T4 DNA ligase (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA Magnetic Isolation Module and then multiplexed via PCR amplification using NEBNext® Multiplex Oligos for Illumina® according to the manufacturer’s instructions (New England Biolabs). Library concentration was quantified with Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µg of RNA was used to synthesize cDNA by oligo dT (New England Biolabs) and reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2024Quote: The reaction was brought up to 50 uL with the addition of CutSmart (final concentration 1X) and 1 uL DpnI (NEB, Catalog #R0176S) and incubated for 1 hour at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed ...
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bioRxiv - Systems Biology 2024Quote: ... and 3 μL of NEBNext Ultra II End Prep Enzyme Mix (NEB) were added to each sample and mixed well by pipetting up and down ...
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bioRxiv - Genomics 2024Quote: ... samples were first treated with Antarctic Phosphatase (NEB), followed by treatment with T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A library was then prepared following the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit procedure (New England Biolabs, Ipswich, MA, USA). A library was directly prepared from 100 ng of total RNA extracted from floral buds using the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Gibson assemblies were transformed the assembly mix into DH5α competent cells (NEB #C2987H). Plasmid backbones were digested with restriction enzymes and purified using Qiagen QIAquick gel extraction kit (#28706) ...
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bioRxiv - Evolutionary Biology 2024Quote: The RNA prepared as described above was sent to Macrogen Japan for library preparation and cDNA sequencing as follows: ribosomal RNA (rRNA) was removed from each sample using the NEBNext Bacteria rRNA Depletion Kit (New England BioLabs, Ipswich, MA), and library preparation was performed using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... or Phusion® High-Fidelity DNA Polymerase (New England Biolabs®) were used for reactions where high fidelity PCR amplification was required ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... UDG-treated DNA libraries were re-amplified with NEBNext Q5 Master Mix (NEB) and P5 and P7 primers to a concentration of approximately 30 ng/µL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 µl 1,000 U/µl USER Enzyme (NEB), Ultrapure water (Thermo ...
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bioRxiv - Systems Biology 2024Quote: ... and close circular plasmids were assembled via a single restriction-ligation reaction with BsaI enzyme (R3535L, New England Biolabs) and T4 DNA ligase (M0202L ...
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bioRxiv - Systems Biology 2024Quote: ... 28 cycles) using the Phusion® High-Fidelity PCR Master Mix (NEB, M0531L). Samples were verified using gel electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... the second strand synthesis was carried out utilizing the NEBNext Second Strand Synthesis Kit (New England Biolabs, E6111S).
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bioRxiv - Synthetic Biology 2024Quote: ... PCR fragments were amplified using Q5 polymerase (NEB). Vectors were digested with NEB restriction enzymes and ligated to gel-purified PCR products using T4 ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg of plasmid DNA of ACT1 was linearized using SpeI restriction enzyme (NEB, USA), purified by Phenol:Chloroform extraction and re-suspended in 10 μl of RNase free sterile water (Raut et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Pre-amplified cDNA was treated with exonuclease I (New England Biolabs) and diluted 5- (for BSF ...
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bioRxiv - Molecular Biology 2024Quote: ... 16 µg purified RNA was mixed with 6X gel loading dye (New England BioLabs) and run on a 1% agarose-TAE (20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by DNase I (NEB, USA) treatment for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Riboprobe for the full-length ACT1 gene was made using “HiScribe™ T7 Quick High Yield RNA Synthesis Kit” (NEB, USA) using Fluorescein labelling mix (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed using protoscript II (NEB) and RT primer oBZ408 ...
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bioRxiv - Molecular Biology 2024Quote: Libraries of immunoprecipitated DNA were generated from 3 ng of starting DNA with the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions at the CRG Genomics Core Facility (Barcelona ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested bovine serum albumin (New England Biolabs) was analysed between each sample to avoid sample carryover and to assure stability of the instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... proximity ligation was carried out overnight at 16°C with 10000U of T4 DNA Ligase (New England Biolabs, #M0202M). Chromatin was reverse-crosslinked with 16U of Proteinase K (New England Biolabs ...
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bioRxiv - Systems Biology 2024Quote: ... and 3 μL of Klenow 3’ to 5’ exo (5 U/μL, NEB), and samples were incubated in a thermocycler at 37°C for 30 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The concentrate was combined with 10x modified Qiagen PB buffer as described in (57) and purified using Monarch columns (NEB). After DNA binding to the Monarch columns ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... Approximately 1 kb upstream and downstream of speEB were amplified and assembled via ITA into pMAD digested with BglII and EcoRI (New England Biolabs, R0144 and R3101, respectively), resulting in plasmid pNOBb41 (Table 4) ...
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bioRxiv - Genetics 2024Quote: ... DNA regions for plasmid assembly were identified using Biocyc (41) and amplified by touchdown PCR from the bacterial chromosomes using Q5 DNA polymerase (New England Biolabs, M0492) and oligonucleotides from Integrated DNA Technologies (Coralville ...
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bioRxiv - Genetics 2024Quote: ... An amplicon DNA library was made by designing amplicons to the coding regions of NR1H3 and PCR was undertaken using Q5® Hot Start High-Fidelity 2X Master Mix (Catalogue number M0494S, New England Biolabs® Inc) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... These amplicons were assembled in pDG1664 digested with EcoRI and HindIII (New England Biolabs, R3101 and R3104, respectively) by ITA and were expressed under the control of their respective native promoters ...
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bioRxiv - Genetics 2024Quote: ... We treated DNA extracts with USER (NEB) during library preparation to cut DNA at uracils ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli dam-/dcm- was purchased from New England BioLabs (NEB) and used for de-methylation of plasmid DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting ligated products were used to transform 50 uL of electrocompetent cells (NEB 10Beta ...