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Citations for New England Biolabs :
6551 - 6600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Each strain was streaked for isolation and PCR was performed with each strain using Taq DNA polymerase (NEB) and the primers and reaction conditions listed in Table S7A to amplify one 610-bp region of the eps locus (NE91_04885) ...
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bioRxiv - Microbiology 2024Quote: ... Isolation of mRNA was achieved via poly(A) enrichment before library preparation (Ultra II Directional RNA library prep kit, New England Biolabs). Libraries were sequenced on an Illumina NovaSeq with 40M 150bp paired end reads per sample.
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with Phusion HF DNA polymerase (NEB) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2024Quote: ... a 30 uL aliquot of lysate was transferred to a clean 1.5 mL microcentrifuge tube and incubated with 1 uL Endo-H (NEB) for 45 min in a 37C bead bath ...
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bioRxiv - Microbiology 2024Quote: ... using the 2X Gibson Assembly Kit (Cat # E2611S, New England Biolabs). Assembled constructs were confirmed by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA purification for use in cloning or whole-genome sequencing was purified using the Monarch HMW DNA Extraction Kit for Tissue (T3060L, New England Biolabs) according to manufacturer’s instruction with one modification ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic DNA inserts were cloned into our plasmid pZE21-ME using 2x NEBuilder HiFi assembly mix (New England Biolabs, E2621L) according to manufacturer instructions (assuming an insert DNA mass of 15 ng and an average fragment size of 2 kb) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were extracted from each strain by miniprep kit (New England Biolabs, T1010L) and whole plasmid sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Microbiology 2024Quote: ... The 12 μl of size-selected metagenomic DNA fragments were gap-filled by the addition of 12 μl of 2x Q5 DNA polymerase (New England Biolabs, M0492S) (pre-incubated at 98°C for 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ... coli DH10B electrocompetent cells (New England Biolabs, C3020K) by electroporation at 1.8 kV in 0.1 mm cuvettes ...
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bioRxiv - Microbiology 2024Quote: ... All three libraries were rescued post-transformation by inoculation into 1 ml of SOC outgrowth medium (New England Biolabs, B9020S) pre-warmed to 37°C and incubated with aeration at 37°C for 1 hour.
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bioRxiv - Microbiology 2024Quote: ... and held at 72°C for 15 minutes before purification by PCR and DNA cleanup kit (New England Biolabs, T1030S) according to manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... The purified fusion construct was ligated into SmaI-digested pIMAY with Quick Ligase (NEB) and transformed into chemically competent E ...
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bioRxiv - Microbiology 2024Quote: ... where P3 includes homology to P2 (Phusion polymerase, NEB). Overlap extension PCR with primers P5 and P6 set ∼100 bp inside of P1 and P4 ...
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bioRxiv - Microbiology 2024Quote: ... digested with SmaI (NEB), and treated with shrimp alkaline phosphatase (rSAP)(NEB ...
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bioRxiv - Microbiology 2024Quote: ... The purified construct was phosphorylated with T4 polynucleotide kinase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Large colonies were screened with colony PCR (Phusion polymerase, NEB) for integration through the 5’ flank using primers P1 and P6 ...
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bioRxiv - Microbiology 2024Quote: The sgRNA-containing region of the lentivirus in the sample DNA was PCR amplified using NEBNext Ultra II Q5 Master Mix as per manufacturers’ instructions (New England Biolabs), and with primers (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) and qPCR performed using iTaq Universal Probes Kit (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... which was fused to codons 247-1334 of hylA amplified via AOJ_731 and AOJ_732 by digesting both DNA fragments with BspQI (NEB) and ligation using T4 DNA ligase.
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bioRxiv - Microbiology 2024Quote: ... Mutagenesis of RBK21 constructs was carried out using the Q5 Site Directed Mutagenesis kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2024Quote: ... Size-selected cDNA was then PCR amplified for 12 cycles using Q5 High-fidelity polymerase (NEB #M0491S). Amplified libraries were then run on a 6% TBE gel ...
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bioRxiv - Immunology 2024Quote: ... ΔPYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-ΔPYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Microbiology 2024Quote: ... SINV-REP construct derived from pToto64 clone encoding SINV non-structural proteins and a reporter luciferase tag was linearized using SacI (New England Biolabs), followed by in vitro transcription using SP6 RNA polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (NEB). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Microbiology 2024Quote: ... the purified genomic DNAs were analyzed by restriction endonuclease digestions (Bsiw I for Kp7, Age I for Kp9, and BspQ I for Kp11) (NEB Cat. R0553V, R0552V and R0712S). Each reaction contained 500 ng of the purified genomic DNA and the corresponding enzyme and was incubated for 1 hour at 55 □ for Bsiw I ...
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bioRxiv - Microbiology 2024Quote: ... treated with DNase I (NEB), and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... All plasmids were checked by enzymatic digestion (New England Biolabs). Plasmid DNA was extracted from 3 mL of overnight E ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli DH5lll bacteria (New England Biolabs), used for plasmid constructions ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM ribonucleoside-vanadyl complex (New England Biolabs, Beverley, MA, USA), and 1× complete EDTA-free protease inhibitor (Roche ...
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bioRxiv - Microbiology 2024Quote: ... The in situ observation of RNA digestion by RNase was executed through introducing a 0.2 U/µL of ShortCut RNase III (New England Biolabs) to the AFM liquid cell during imaging ...
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bioRxiv - Microbiology 2024Quote: ... purified using the Monarch DNA Gel Extraction kit (NEB) and ligated using T4 DNA ligase (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... each pair of oligonucleotides was annealed and phosphorylated with T4 PNK (New England Biolabs, # M0201) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Microbiology 2024Quote: The in vitro transcription assay was conducted using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... All PCRs were performed using Q5 high-fidelity DNA polymerase (NEB). Plasmids with desired mutation were cloned in Escherichia coli DH5α ...
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bioRxiv - Microbiology 2024Quote: ... was digested with XbaI and MluI restriction enzymes and barcoded linear HA libraries were inserted into the lentivirus backbone using NEB HiFi assembly kit (NEB E5520S). Assembled plasmid libraries were then electroporated into electrocompetent 10b cells (NEB C3020K ...
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bioRxiv - Microbiology 2024Quote: ... Assembled plasmid libraries were then electroporated into electrocompetent 10b cells (NEB C3020K) and incubated overnight on ampicillin containing LB plates ...
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bioRxiv - Microbiology 2024Quote: ... HiFi DNA assembly cloning kit (New England Biolabs (NEB)) was used according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... The HiFi Assembly reaction master mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2024Quote: ... The products of the HiFi reaction were transformed into NEB10β competent cells (NEB) and plated on LB agar with appropriate antibiotics ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Ultra II DNA Library prep Kit (E7370; NEB). Libraries were sequenced using an Illumina NextSeq 2000 sequencer 75 bp Single End.
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed by PCR with Illumina P5 and P7 primers using Q5 High-Fidelity DNA Polymerase (#M0491L, NEB), and purified with 1.5x AMPure XP beads ...
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bioRxiv - Microbiology 2024Quote: ... precipitated using an isopropyl alcohol wash and ethanol and then rehydrated in Elution Buffer (New England Biolabs, Whitby, Canada). DNA concentration was measured using a QuBit 4 Fluorometer and the QuBit dsDNA High Sensitivity Quantitation kit (Invitrogen ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... single colonies were picked and used as a template for amplification by PCR using Q5 DNA polymerase (New England Biolabs, Whitby, Canada) with primers CR1_F and R as well as CR3_F and R (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...