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Citations for New England Biolabs :
6501 - 6550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Golden Gate assembly was used to clone sgRNAs into the desired backbone using BsaI (BsaI-HF v2, New England Biolabs, NEB) (26) ...
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bioRxiv - Genetics 2024Quote: ... and then transformed into 10-beta competent cells (NEB, C3020) via electroporation ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly was performed using the master mix (New England Biolabs, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Golden Gate assembly was used to clone sgRNAs into the desired backbone using BsaI (BsaI-HF v2, New England Biolabs, NEB) (26) ...
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bioRxiv - Genetics 2024Quote: ... 10 µL Proteinase K (NEB #P8107, 20 mg/ml), and 10 µL 1M DTT (GoldBio #DTT in dH2O ...
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bioRxiv - Genetics 2024Quote: All plasmids were transformed into NEB 5-alpha Competent Escherichia coli cells (New England Biolabs) and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN) ...
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bioRxiv - Genetics 2024Quote: ... Q5 Hot Start High-Fidelity 2X Master Mix (NEB., Cat#M0494) was used to amplify the genomic region using 30 cycles of amplification with 20ng of total genomic DNA per reaction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Genetics 2024Quote: ... we prefer to use New England Biolabs (NEB) SspI-HF ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Constructs were assembled using homology based cloning methods (HiFi DNA Assembly, NEB Australia) with a combination of synthesized DNA and PCR products with primers to introduce overhangs or sequence modifications ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB Cat#C2992H). The necessary dsDNA fragments were ordered from Twist Bioscience.
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bioRxiv - Genetics 2024Quote: ... as well as a number of buffers customarily used for restriction enzyme reactions sourced from NEB. We found that all NEB buffers containing Mg ions were not suitable for the recombination reactions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3% dimethyl sulphoxide (DMSO) and 0.4 U of Taq-Phusion High-Fidelity (New England Biolabs, USA). PCR conditions were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, E7490) was employed to capture mRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank: NM_008590; Integrated DNA Technologies) was inserted with NEBuilder HiFi DNA assembly (New England Biolabs). The final construct was verified by plasmid DNA sequencing (Plasmidsaurus).
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bioRxiv - Developmental Biology 2024Quote: ... This fragment was generated by Gibson assembly (New England Biolabs) using synthetized gene fragments (IDT technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA was enriched with magnetic oligo (dT) beads (New England Biolabs, cat#S1419S). The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, cat#E7530L). Sequencing was performed by a paired-end 125 cycles rapid run on the Illumina HiSeq2500 ...
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bioRxiv - Cell Biology 2024Quote: ... the newly produced CENP-A-SNAP pool was labelled by exposing the mitotic cells to media containing 3 µM SNAP-Cell 647 (NEB) and 5 µM STLC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... existing CENP-A-SNAP proteins were labelled for 30 min using 10 µM SNAP-Cell Block (NEB) and cells were exposed to media containing 5 µM S-trityl-L-cysteine (STLC - Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... in the presence of RNase inhibitor (NEB, M3014) at 37°C for 45 min ...
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bioRxiv - Genetics 2024Quote: ... the Cas9 terminator (TCYC1) was switched to an ADH1 terminator (TADH1) by Gibson assembly using the HiFi DNA Assembly kit (New England Biolabs). Cas9D10A ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were performed using Phusion High-Fidelity PCR kit (New England Biolabs #M0530L) according to manufacturer’s protocol using 50 ng template DNA per 100µL reaction and 18 cycles ...
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bioRxiv - Genetics 2024Quote: ... pT312 (ccdB+) was digested with BsaI-HF-v2 (New England Biolabs) and the vector was gel purified ...
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bioRxiv - Genetics 2024Quote: ... An additional 20-minute digestion was then performed with 5 µL RNase A (NEB #T3018, 20 mg/ml). The rest of the protocol remained unchanged ...
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bioRxiv - Developmental Biology 2024Quote: ... Exon 9 of daf-2 was amplified with Phusion High-Fidelity DNA polymerase (New England Biolabs, M0530S) using primers F_Primer ...
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bioRxiv - Developmental Biology 2024Quote: ... Ligation was performed using T4 DNA ligase (New England Biolabs, M0202S) and the resulting plasmid was transformed into the HT115 E ...
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bioRxiv - Developmental Biology 2024Quote: ... and NcoI (New England Biolabs, R0193S) restriction enzymes ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplicon and L4440 vector were digested using ApaI (New England Biolabs, R0114S) and NcoI (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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bioRxiv - Developmental Biology 2024Quote: ... and subjected to library construction using Q5 high-fidelity DNA polymerase (New England Biolabs, M0544S). For high-throughput sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng total RNA was used as template with the Luna Universal One-step RT-qPCR kit (New England Biolabs, E3005L). For each condition 3 biological replicates with two technical replicate RT-qPCR reactions were run on a CFX96 Connect Real-Time System (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Poly-A selection for mRNA enrichment was performed using the corresponding NEB kit (#E7490) followed by library preparation using the directional RNA library prep kit from NEB (#E7765). An additional NEB kit Low Input RNA library prep kit (#E6420 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and all fragment ligation reactions were performed using NEBuilder HiFi DNA Assembly Master Mix (#M5520AA, New England Biolabs).
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bioRxiv - Evolutionary Biology 2024Quote: ... Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes) and ligation at 16 °C (4 minutes) with 400 U of T4 ligase (New England Biolabs, USA), followed by heat-inactivation at 65 °C (10 minutes) ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosome-depleted RNA was used to make cDNA libraries using NEBNext Ultra II RNA Library preparation kit for Illumina and NEBNext Multiplex Oligos for Illumina as per manufacturer’s protocol (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... NEBNext Multiplex Oligos (UMI Adaptor RNA Set 1, NEB #E7335L) and NEBnext Ultra II Directional RNA library prep kits (NEB #E7760L) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted for 3min at 2000rpm and then resuspended into spheroplast buffer (1.2 M sorbitol, 100 mM KHPO4, pH = 7.5, 20mM ribonucleoside-vanadyl complex (NEB # S1402S), 20mM B-mercaptoethanol) ...
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bioRxiv - Cell Biology 2024Quote: ... treated with DNase I (NEB #M0303L) and purified using an NEB clean and concentrate kit (NEB #T2030).
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bioRxiv - Cell Biology 2024Quote: ... The purified RNA was then treated with Dnase I (NEB) before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Cell Biology 2024Quote: ... The IVT RNA was produced by first amplifying a linear DNA fragment encoding NanoLuc using Q5 polymerase (NEB #M0494S), and purifying the DNA using an NEB clean and concentrate kit ...
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bioRxiv - Cell Biology 2024Quote: ... before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Cell Biology 2024Quote: ... The RNA was then made using a T7 Highscribe kit (NEB #E2040S), treated with DNase I (NEB #M0303L ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEB NEBNext Ultra II (NEB #E7760 ...
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bioRxiv - Cell Biology 2024Quote: ... and purified using an NEB clean and concentrate kit (NEB #T2030).
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.75 µL DpnI (New England Biolabs). The reactions were incubated at 37°C for 20 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 mM Tris-acetate, 10 mM magnesium acetate, 100 µg/mL recombinant albumen at pH 7.9; New England Biolabs) and 0.75 µL DpnI (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid construction was conducted using the isothermal Gibson Assembly method and New England Biolabs (NEB) Gibson Assembly Master Mix (#E2611) ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by digestion with RNase I (Thermo AM2294) and RNase H (NEB M0297) and purification with the DNA Clean & Concentrator - 5 (Zymo D4014) ...
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bioRxiv - Synthetic Biology 2024Quote: All DNA manipulations were performed using NEB Turbo cells (New England Biolabs) or Mach1F cells ...