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Citations for New England Biolabs :
6401 - 6450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and T4 DNA ligase were purchased from New England BioLabs (NEB). DpnI and T4 Polynucleotide Kinase were purchased from ThermoFisher Scientific.
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bioRxiv - Synthetic Biology 2024Quote: ... 20 U BsaI-HFv2 (NEB), and 400 U of Salt-T4 DNA Ligase (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... All fragments were digested with DpnI (NEB) overnight at 37 °C in the PCR buffer and PCR purified using the NEB Monarch® PCR & DNA Cleanup Kit the next day ...
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bioRxiv - Synthetic Biology 2024Quote: All the integrative plasmids generated in this study were linearized using NotI (New England Biolabs) and the DNA fragments were transformed in yeast together with relevant gRNA plasmid from EasyClone-MarkerFree (Jessop-Fabre et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... 30 uL of the purified PCR amplicons were then digested and ligated in a 50 uL one-pot reaction containing 1x T4 ligase buffer (NEB), 20U DpnI (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20U DpnI (NEB), 20 U BsaI-HFv2 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was reverse-crosslinked with 16U of Proteinase K (New England Biolabs, #P8107S) and 100 ug RNAase A (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 4x106 JM8+/+ and Bmal1-/- cells were fixed with formaldehyde 1% and digested overnight at 37°C using 400U of MboI (New England Biolabs, #R0147M). After filling with 50nM biotin-dATP (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50U of Klenow polymerase (New England Biolabs, #M0210M), proximity ligation was carried out overnight at 16°C with 10000U of T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... + 2 μL BlpI (NEB cat# R0585S) + nuclease-free water to 50 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μL NheI-HF (NEB cat#R3131S), 5 μL CutSmart 10x buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (High Efficiency) (NEB cat#C2987H) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... ssSynth primer was extended by adding 1 μL 10mM dNTP’s (Fisher cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S) then incubating at 37°C for 1hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Genes of interest were cloned into Bxb1 attB-containing plasmids using standard Gibson assembly cloning techniques (NEB cat #E2621L). To stably recombine the attB plasmids under control of the landing pad promoters ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL Klenow polymerase (NEB cat#M0210S) was added to the annealed oligos ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL dNTP’s (10mM each, NEB cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S ...
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bioRxiv - Molecular Biology 2024Quote: ... + 13.75 μL nuclease-free water + 1.5 μL DMSO + 25 μL Q5® Hot Start High-Fidelity 2X Master Mix (NEB cat #M0494). Cycling included 98°C for 30s ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 rounds of PCR were run using Q5® Hot Start High-Fidelity 2X Master Mix (NEB cat #M0494) following the manufacturer’s instructions with a 63°C annealing temperature and 30s extension ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (High Efficiency) (NEB cat#C2987H), transformants enumerated ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... fragments were dephosphorylated by adding 5 μL antarctic phosphatase buffer and 2 μL enzyme (NEB cat#M0289S) and incubating for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μL AflII (NEB cat#R0520S), 1.5 μL NheI-HF (NEB cat#R3131S) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from bulk liver tissue using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) after homogenization with steel beads using a TissueLyser LT bead mill (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL 2x HiFi DNA Assembly master mix (NEB cat#E2621L). After incubation ...
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bioRxiv - Molecular Biology 2024Quote: dsBC was cloned into the digested vector by combining 200ng vector (∼0.035pmol, 8uL), 10ng dsBC (∼0.175pmol, 2uL) and 10 μL 2x NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L) and incubating at 50°C for 1hr ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 μL NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L), and nuclease-free water to 80uL ...
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bioRxiv - Molecular Biology 2024Quote: The Q5 Site-Directed Mutagenesis Kit (New England Biolabs) was used to introduce the mutations into pET15b plasmid-borne copies of PRX1 and TRX3 genes using pairs of adequate ...
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bioRxiv - Systems Biology 2024Quote: ... Quantitative PCR was performed with Luna Universal qPCR Master Mix (New England BioLabs) using an Applied Biosystems ViiA 7 Real-Time PCR system ...
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bioRxiv - Molecular Biology 2024Quote: ... We used Template Switching RT Enzyme Mix (NEB, Ipswich, MA), along with a uniquely barcoded oligo(dT)30 primer for each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from libraries was prepared according to the instructions of the “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (New England Biolabs), following the “Poly(A ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X Thermo PCR buffer (NEB), 2 µL of ES-E14 wildtype cDNA template or from 1 µL plasmid pLJM1-EGFP for GFP esiRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added to the reaction and incubated at 37°C for 1hr ...
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bioRxiv - Molecular Biology 2024Quote: Barcoded variant libraries were digested overnight with SpeI-HF (NEB cat # R3133S) and NheI-HF (NEB cat# R3131S ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB, #T1030L) prior to Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic regions flanking the expected cut sites for the indicated sgRNAs were PCR amplified using Q5 PCR Master Mix (NEB, #M0541L). PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 μL of HpaII enzyme (NEB, #R0171L), 0.05 μL of DraI enzyme (NEB ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was harvested from sgRNA-transduced cells using either the Monarch genomic DNA purification kit (NEB, #T3010S) or QuickExtract DNA extraction solution (Lucigen ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 μL of Hot Start Taq DNA Polymerase (NEB, #M0495L), and 2.45 μL of nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2X NEBNext Q5 high fidelity master mix (NEB, #M0541L) as previously described25,71 ...
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of 10x T4 ligase buffer (NEB B0202S), 1 µl of T4 PNK ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 400 U of Salt-T4 DNA Ligase (NEB). All reactions were then incubated at 37 °C 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... We used Phusion High-Fidelity PCR MasterMix with HF Buffer (NEB, Catalog #M0531L) for the second PCR and used the following PCR conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of ExoI and 1 µl rSAP (NEB) were added to the recovery products and incubated for 15 min at 37°C followed by heat inactivation of 20 min at 80°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli strains NEB Stable and NEB 10β (NEB) were cultured in Luria–Bertani (LB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells (NEB). The cells were freshly prepared to maximise transformation efficiency ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid assembly was performed using the HiFi DNA Assembly (New England Biolabs) according to the supplier’s instructions ...