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Citations for New England Biolabs :
6351 - 6400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Two cycles of whole genome amplification were performed using 50 U of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), dNTP solution mix (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: Double-stranded DNA libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolab: NEB). Around 1 ng of DNA was used for each library ...
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bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
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bioRxiv - Microbiology 2024Quote: ... Then amplicons were further treated with DpnI (New England BioLabs, Ipswich, Massachusetts, United States). E ...
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bioRxiv - Genomics 2024Quote: ... All rmp loci were amplified from genomic DNA by PCR and then cloned into pMWO-07829 by Gibson assembly (NEB). The rmp expression vectors were then transformed into KPPR1S Δrmp ...
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bioRxiv - Genomics 2024Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were generated using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Mutations M1 (CAGGTGcAac) and M2 (CAGGTGTACA) in pX_Luc were introduced using Q5 site-directed mutagenesis kit (New England Biolabs). Plasmids expressing DYRK1A or DYRK1A_KR under the control of the CMV promoter were previously described [29].
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used for the following RNA-seq library preparation according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was carried out from 100ng of total RNA with NEBNext bacterial rRNA depletion kit (NEB#e7850). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Immunology 2024Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesized using the ProtoScript First-Strand cDNA Synthesis kit (New England Biolabs). Real-time PCR assays were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Genomics 2024Quote: ... The purified Hi-C DNA was sheared by covaries E220 evolution and libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, no. E7645), and the average size of the library was detected by bioanalyzer ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of gDNA was digested with TaqaI (NEB Ipswich, MA) at 65 ⁰C for 2 h followed by MspI treatment (NEB Ipswich ...
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bioRxiv - Microbiology 2024Quote: Approximately 1×107 bacterial cells were centrifuged at 12,000 x g for 1 minute before beginning RNA extraction as per the Monarch Total RNA Miniprep kit protocol (New England Biolabs). RNA was eluted in 30 μL nuclease-free water (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified using the Monarch DNA and PCR Cleanup Kit (New England Biolabs) and precipitated as per the Co-Precipitant Pink (Bioline ...
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bioRxiv - Genomics 2024Quote: ... oligos with 5’ phosphate were further phosphorylated by incubating them (at a concentration of 50 μM for indices and 25 μM for BL092-50nt) with 10U T4 polynucleotide kinase (NEB) in 1X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... spin purified with DNA clean and concentrate kit (NEB), and sequenced with Plasmidsaurus.
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bioRxiv - Microbiology 2024Quote: ... 9.5 µL of tRNA was combined with 2 U/µL of T4 PNK (NEB, M0201S) was well as N-terminally His6-TEV-tagged ATfaRel SAH (final concentration 1 µM ...
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bioRxiv - Microbiology 2024Quote: ... MBP alone was expressed from the pMAL vector (NEB #N8108) and purified according to the manufacturer ‘s recommendations.
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bioRxiv - Microbiology 2024Quote: ... were performed as per the manufacturer’s instructions using 10 ng/µL of DHFR-Strep reporter plasmid with the addition of 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified FaRel2-FLAG3 (at 50 nM as final concentration ...
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bioRxiv - Microbiology 2024Quote: ... 200 U T4 RNA ligase 2 truncated KQ (NEB), 1x T4 RNA ligase buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5 μM Mth RNA ligase (NEB), 1 x adenylation buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 1x T4 RNA ligase buffer (NEB), 25% PEG8000 ...
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bioRxiv - Microbiology 2024Quote: ... 1 x adenylation buffer (NEB), and 1 mM ATP ...
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bioRxiv - Microbiology 2024Quote: ... One micro litter of circularized cDNA was subjected to PCR in a 20 μL reaction mixture composed of 20 U/mL Phusion polymerase (NEB), 1x Phusion GC buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress In Vitro Protein Synthesis Kit (NEB, E6800) were performed as per the manufacturer’s instructions using 10 ng/µL of DHFR-Strep reporter plasmid with the addition of 0.8 U/µL RNase Inhibitor Murine (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were purified and assembled using Gibson Assembly (NEB).
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bioRxiv - Microbiology 2024Quote: ... and utilized for library constructions with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina along with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; NEB; # E7760L). The libraries were sequenced on the NovaSeq platform as paired-end reads using the S1 v1.5 kit with 300 cycles (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were assembled using Gibson Assembly (NEB).
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA was treated with RNase-free DNase I (New England Biolabs [NEB], Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... we prepared genomic libraries with the NEBNext Ultra FS II kit (New England Biolabs) and resequenced the whole genomes at the SNP&SEQ Technology Platform in Uppsala ...
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bioRxiv - Genomics 2024Quote: ... 5 ng of the resulting RNA was applied for library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs).
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bioRxiv - Genetics 2024Quote: ... NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) was used following the manufacturer’s protocol to recombine the fragments ...
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bioRxiv - Genetics 2024Quote: ... and CutSmart buffer (New England Biolabs) up to 30 μL and digested overnight ...
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bioRxiv - Immunology 2024Quote: ... and 0.5% NP-40 (NEB) (3 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cat. No. B6002S]; 2.5 µl 100 µM gRNA [ordered as a synthesized oligo]; 2 µl 100 µM LbaCas12a [NEB, Cat. No. M0653T]) was incubated for 5 minutes at room temperature.
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bioRxiv - Evolutionary Biology 2024Quote: ... and using the NEBNext® Multiplex Oligos for Illumina (New England Biolabs). Library amplification was carried out with the KAPA HiFi DNA polymerase (Roche) ...
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bioRxiv - Evolutionary Biology 2024Quote: 3 µl of cDNA samples or ssDNA standards were added to a 20µl reaction with Luna Universal qPCR Master Mix (NEB, Cat, No. M3003) according to the provided protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RRBS libraries from the common garden experiment were created by adapter-ligating and end-repairing MspI digested fragments following the NEBNextUltra protocol (New England BioLabs). Bisulfite conversion was performed with the EZ DNA Methylation Gold Kit (Zymo ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Then total RNA (500 ng per sample) was purified by one round of polyA mRNA selection with oligo-dT magnetic beads (NEB, Cat. No. S1550S), converted to cDNA using KAPA mRNA HyperPrep kit (KAPA biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each plate’s DNA library was then amplified in two 50 µl Q5 High-Fidelity DNA Polymerase reactions (New England BioLabs) for 11 thermocycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were split into two equal samples and incubated at 50°C for 25 minutes with exonuclease 1 (NEB, M0293) to remove unintegrated barcoded transposon adapters ...
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bioRxiv - Immunology 2024Quote: ... and the cDNA was generated using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, #E7770S). Next ...
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bioRxiv - Cell Biology 2024Quote: ... The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs) for 1h at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 pmol forward oligo and 100 pmol reverse oligo were resuspended in 25 μL 1x NEBuffer 2 (NEB, B7002S). The solution was incubated in a 95 °C water bath for 4 min then slowly cooled (∼0.5 °C/min ...
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bioRxiv - Cell Biology 2024Quote: ... and ligated with the oligo solution according to the NEB Quick Ligase (NEB, M2200) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The partial AtVPS13M1 gene was amplified by PCR in 45 cycles using LongAmp Hot Start Taq 2X Master Mix (New England Biolabs), from 30/40ng of cDNA per sample in reaction volume of 100µl ...
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bioRxiv - Cell Biology 2024Quote: ... together with the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490) and NEBNext Multiplex Oligos for Illumina (#E7600) (New England Biolabs). RNA-seq libraries were processed on a Biomek i7 Hybrid instrument (Beckman Coulter ...