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Citations for New England Biolabs :
6301 - 6350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Selections were then purified using the Monarch® PCR & DNA Cleanup Kit (NEB), eluting in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: All DNA constructs and oligos were designed using either Snapgene (BioMatters Ltd) or Primer3 (Koressaar and Remm 2007) and plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lettuce variant sequences were cloned into retron Eco2 plasmid using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primer sequences and annealing temperatures were generated using the NEBaseChanger™ tool.
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bioRxiv - Synthetic Biology 2024Quote: ... The 2A-tagged ZFs were subsequently inserted into expression vectors with EF1a and BGH polyA signal via Golden Gate assembly with BsaI (NEB Stable).
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bioRxiv - Synthetic Biology 2024Quote: ... coli Ribosome (NEB), 5 nM reporter protein expression plasmids (either PT7-MGA-UTR1-deGFP or PT7-UTR1-deGFP),2.5x Energy Mix (125 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA strands were transcribed in-house and column-purified using the Monarch® RNA Cleanup Kit (New England Biolabs). The concentration of oligonucleotides was quantified by UV-vis spectrophotometry using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Monarch® RNA Cleanup Kit (New England Biolabs) was used to purify transcribed RNA strands ...
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bioRxiv - Synthetic Biology 2024Quote: ... and T4 DNA ligase (T4 Ligase, NEB). Ligations were transformed in chemically competent E ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pYFAC-ODC-pyrG were built by isothermal assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, MA, USA). First ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 U/ml of RNase-free DNase I (New England Biolabs, USA), 0.5 U/μl of SUPERase•In RNase Inhibitor (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 3′ end of isolated RNA was subsequently dephosphorylated using T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 hour based on the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 25% PEG-8000) into MilliQ and supplementing with 5.3 U/mL T5-exonuclease (NEB), 33.3 U/mL Phusion DNA polymerase (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... extracted PCR product with 2 μl of NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the amplified plasmid DNA was extracted using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplified products were digested with DpnI (NEB) and purified with the Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Systems Biology 2024Quote: ... total-RNA was polyA selected using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490S). Libraries were sequenced at low-scale (approx ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Synthetic Biology 2024Quote: ... The KODΔ mutant was generated using primers PH_Delta_KOD_F1/R1 (Table S1) through iPCR and cloned into the final BL21(DE3) (NEB) strain following the same procedure as the library construction.
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bioRxiv - Synthetic Biology 2024Quote: Mutants were re-transformed into BL21(DE3) (NEB) cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutants were generated through inverse PCR (iPCR) on the pET23-KOD-Exo-(Table S2) plasmid in Q5 High-Fidelity DNA Polymerase (NEB) reactions of 30 cycles following the manufacturer’s standard protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning was performed using restriction enzymes and HiFi DNA assembly (NEB). Primers (Eurofins Genomics ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 µl of DNase I (2500U/mL, NEB) for each mL of B-PER reagent used ...
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bioRxiv - Systems Biology 2024Quote: ... Resulting cDNA was amplified by 19 PCR cycles using LongAmp Hot Start Taq 2X Master Mix (NEB). RT and PCR primers were used as previously described (Rabani ...
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bioRxiv - Systems Biology 2024Quote: ... PCR also introduced specific i5 (NEB, 7780) and N8 (Vazyme ...
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bioRxiv - Systems Biology 2024Quote: ... HiScribe® SP6 RNA Synthesis Kit (NEB) was used to in-vitro transcribe a library of mRNA reporters from linearized plasmids ...
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bioRxiv - Systems Biology 2024Quote: ... The material was made into blunt-end dsRNA products with 15U T4 DNA polymerase (NEB, USA) for 20 minutes at 16°C and reactions stopped by addition of 20mM EDTA (pH 8.0 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligodeoxynucleotides (Table S2) for detection of specific RNA species were end-labeled with [γ- 32P]-ATP using T4 polynucleotide kinase (PNK) (NEB). Prehybridization and hybridization were performed in Church buffer [0.5 M phosphate buffer (pH 7.2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by a DpnI (NEB) digest prior ligation ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 units (U) of thermostable inorganic pyrophosphatase (New England Biolabs, catalog no. M0296S), 100 nM transcription template ...
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bioRxiv - Synthetic Biology 2024Quote: Escherichia coli strain K12 (NEB Turbo Competent E ...
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bioRxiv - Synthetic Biology 2024Quote: ... where separately introduced to the wild-type MS2 coat protein present in these mRNA constructs using the NEB-Q5 mutagenesis kit (New England Biolabs). The primer sets and PCR settings used are listed in supplementary information ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 U RNase inhibitor (NEB, #M0314S) were added to the initial titration of lysate in IVT ...
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bioRxiv - Synthetic Biology 2024Quote: ... The level 0 acceptor plasmid was amplified with the primer pair SLo0765/SLo0766 using Q5 DNA Polymerase (NEB) followed by a DpnI (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: Escherichia coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs, catalog no. C2984) was used for routine cloning ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.015 U/µL thermostable inorganic pyrophosphatase (New England Biolabs) and 15 nM DNA template ...
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bioRxiv - Synthetic Biology 2024Quote: ... sequences were cloned into a human U6 (hU6)-driven expression plasmid that also contains a CMV-driven GFP transfection reporter using HiFi DNA Assembly (New England Biolabs). All plasmids were sequence-verified by whole plasmid Nanopore sequencing (Primordium ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were performed using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) ...
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bioRxiv - Systems Biology 2024Quote: ... Purple (NEB, Cat. No. B7024) and 7.8 µl MilliQ water (18 µl final volume) ...
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bioRxiv - Systems Biology 2024Quote: ... IVT products were digested with Shortcut RNase III (NEB) and purified using the PureLink RNA Mini Kit (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... amplicons were indexed using xGen UDI Primer Pairs (10 mM) in 12 µL reactions using the NEB Q5 Hot Start HiFi 2X master mix (New England BioLabs, Ipswich, MA) with the following parameters ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of T4 DNA Polymerase (3 U/μL, NEB) 1 μL of Klenow DNA Polymerase (5 U/μL ...
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bioRxiv - Systems Biology 2024Quote: ... and 0.5 µL of Taq Polymerase (NEB), and the following PCR reaction was performed ...
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bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of Klenow DNA Polymerase (5 U/μL, NEB) and 2 μL of T4 PNK (10 U/μL ...
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bioRxiv - Systems Biology 2024Quote: ... 7 μL of NEBNext Ultra II End Prep Enzyme buffer (NEB) and 3 μL of NEBNext Ultra II End Prep Enzyme Mix (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 μL of Phusion Polymerase (2 U/μL, NEB) were added and mixed by pipette ...
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bioRxiv - Systems Biology 2024Quote: TT-seq libraries were built using the NEBNext Ultra II RNA Library prep kit for Illumina (NEB) according to the manufacturer’s recommendations ...