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Citations for New England Biolabs :
401 - 450 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of the PX330 Cas9 plasmid was digested with BbsI (NEB) following manufacturer’s protocol and purified by agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... with 5 µg/mL restriction enzymes BamHI and NheI (New England Biolabs) according to the manufacturer’s instructions for 1 hour at 37ºC ...
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bioRxiv - Developmental Biology 2023Quote: 40 pg of the final construct was then co-injected together with the I-SceI meganuclease enzyme (NEB) into one-cell stage AB wild type embryos ...
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bioRxiv - Genetics 2024Quote: ... along with the co-injection marker rol-6(su1006) (pRF4) and DNA ladder (1kb DNA Ladder N3232, NEB), generating the three AFD-specific independent lines BFF95 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... Endogenous proteins were captured onto protein G-magnetic beads (NEB; #S1430S), washed extensively in IP buffer and used for POT1wtand POT1V29L source ...
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bioRxiv - Microbiology 2023Quote: ... with the inclusion of m7G(5’)G RNA Cap Analog (NEB). Electroporation of the resulting RNA was carried out using a Neon® Transfection System (Invitrogen™ ...
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bioRxiv - Biophysics 2023Quote: ... 1μL EDTA (0.5m) and 100μL Orange G dye (New England BioLabs). The samples were at 50V for 5min followed by 300V for 25min ...
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... We washed 80µL Protein A/G Magnetic Beads (New England Biolabs) per sample with bead washing buffer (PBS with 0.1% BSA and 2mM EDTA ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions conducted at UR-MAI consisted of 50 μl reactions: 5 μl 10X Standard Taq Buffer (NEB), 2.5 μL of each primer ...
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bioRxiv - Biophysics 2020Quote: ... we used an alternative strategy that enables us to propagate expression plasmids in standard bacterial strains (NEB 5-alpha Competent E ...
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bioRxiv - Bioengineering 2021Quote: A standard PCR process was performed using Q5 high-fidelity DNA polymerase from NEB (cat. no. M0491S). All PCR primers were purchased from IDT (Supplementary Table S1) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1X OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0486L), and 2 μl of water suspended colony ...
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bioRxiv - Genomics 2021Quote: ... Standard RNA Synthesis was carried out with the HiScribe T7 High Yield RNA Synthesis kit (NEB E2040S) for 1.5 hours according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were performed using OneTaq Quick Load 2X Master Mix with Standard Buffer (New England Biolabs). Detection of Mincle-specific cDNA was conducted by PCR with the same gene-specific primers described in the previous section (Suppl ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using OneTaq Quick-Load 2X Master Mix with Standard Buffer (New England Biolabs, USA). DNA assembly was done by using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions were run on 1.2% TAE agarose gel with a standard 1kb ladder (New England BioLabs) to confirm formation of products ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Genetics 2024Quote: ... ssDNA was isolated the following day per M13KO7 helper phage standard manufacturer protocol (NEB cat no. N0315S).
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was purified by standard methods and rRNA depleted using the NEBNext rRNA depletion kit (NEB, as per the manufacturer’s instructions) ...
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bioRxiv - Microbiology 2023Quote: ... 12.5 µl of One Taq Quick-load 2x master mix with standard buffer (New England BioLabs, UK) was aliquoted into a DNase-free 0.2ml transparent PCR tube ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions (15 μL) contained 7.5 μL of 2X OneTaq 2X Master Mix with Standard Buffer (NEB), 5.9 μL H2O ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were stranded using standard N.E.B indices according to manufacturer’s instructions (New England Biolabs, E7730L, E7335L, E7500L). Sequencing reads from all RNA-seq experiments were aligned to hg19 reference genome by STAR v ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were generated using either standard restriction-ligation or assembled using the Gibson Assembly Master Mix (NEB) or In Vivo Assembly ...
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bioRxiv - Immunology 2024Quote: Cloning was performed via standard PCR methods using the Q5 High-Fidelity PCR kit (New England Biolabs), Gibson assembly using the Gibson Assembly® Master Mix (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified plasmid backbone and insert was ligated by T4 ligase reaction following the standard protocol (NEB). The ligated products were transformed into the dSL2 donor cell and selected on the appropriate LB+antibiotic plates.
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bioRxiv - Biochemistry 2024Quote: Sensor constructs were amplified by a standard Q5® High-fidelity DNA-Polymerase protocol (New England Biolabs) using oligonucleotides (Table S6 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (anti-rabbit IgG HRP, 1:5000 dilution, 7074S, NEB) in TBS-T with 1% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting pellet was resuspended in nuclease free water (New England Biolabs) and RNA quantified through measurement of A260 prior to storage at −80 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was treated with RNase free DNase I (New England Biolabs). First-strand cDNA was synthesized using SuperScript III reverse transcriptase (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and treated with RNase-free DNase I (New England Biolabs, Ipswich, MA) for 1 hr at 37°C ...
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bioRxiv - Immunology 2020Quote: The fusion protein was treated with glycerol free PNGase F (NEB, P0705S) in non-denaturing reaction conditions ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting RNA was digested with RNase-free DNase I (NEB, M0303S), and purified with acidic phenol (125:24:1 (phenol:chloroform:isoamyl) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were treated with 1 μl of RNase-free DNase (NEB) to 100 μl of RNA solution and cleaned up with a Monarch RNA cleanup kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was treated twice with RNase-free DNase I (New England Biolabs) for northern blotting analysis and TERRA purification or three times for RT-qPCR analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The RNA was further treated with DNaseI (RNase-free, New England Biolabs) followed by column purification using Monarch Total RNA Miniprep Kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... Residual DNA was degraded using RNase-free DNase I (New England Biolabs) and the in vitro synthesized RNA was purified using the RNA clean-up protocol from RNeasy Mini kit (Qiagen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and nuclease-free H2O up to 20 μL (New England Biolabs, USA). The reaction was performed in 30 cycles of 37 °C for 5 min followed by 16 °C for 5 min and finalized by incubation at 60 °C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extractions were DNase treated (RQ1 RNase-free DNase, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µM i7 index primer and nuclease-free water (New England Biolabs) up to a total volume of 50 µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 U/ml of RNase-free DNase I (New England Biolabs, USA), 0.5 U/μl of SUPERase•In RNase Inhibitor (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... 1987 and treated with RNAse- free DNase I (New England Biolabs®). cDNA was performed using SuperScript™ III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... each 180 μl sample/Saccharibacteria co-culture was treated with Endo Hf Kit P0703L (New England Biolabs, Evry, France): 3 μl of each reagent was added to the sample and incubated for one hour at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... as per the manufacturers protocol and co-transformed into CEN.PK2-1C with pRS425 episomal plasmid backbone digested with SfoI and SacI (NEB). The assembled plasmid was extracted from S ...
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bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
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bioRxiv - Cell Biology 2022Quote: ... purified from Escherichia coli cells using Nucleobond PC100 (MACHERREY-NAGEL GmbH & Co. KG) was treated with the nicking enzyme Nt.BspQI (R0644S, New England Biolabs). The linearized DNA was prepared by digesting the negatively supercoiled DNA with Eco RI (1040A ...
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bioRxiv - Cell Biology 2022Quote: ... The mutated Rop1 expression plasmid and the amber suppressor tRNA plasmid were co-transformed into BL21 (DE3) cells (NEB), as described2 ...