Labshake search
Citations for New England Biolabs :
6251 - 6300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... cells were lysed with glycoprotein denaturing buffer (B1704S, NE BioLabs). Each sample was sonicated ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysates were then treated with 500 U of peptide-N-glycosidase F (PNGase F; P0704L, NE BioLabs) in a reaction solution containing 50 mM sodium phosphate (pH 7.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... and treated with proteinase K (P8107, NE BioLabs) at different concentrations for 10 min at room temperature (R.T.) ...
-
bioRxiv - Cell Biology 2024Quote: ... in a reaction solution containing 50 mM sodium phosphate (pH 7.5) and 1% NP-40 (B2704S, NE BioLabs) for 1 h at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: H in the reaction buffer containing GlycoBuffer 3 (B1720S, NE BioLabs) for 1 h at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... The miRNA:mRNA chimeric ligation step was done at room temperature for 1hr with T4 RNA ligase (NEB). The IP samples were then treated with alkaline phosphatase (FastAP ...
-
bioRxiv - Cell Biology 2024Quote: ... by Gibson assembly (NEB). A DNA mix containing pCZ986 (50 ng/µL) ...
-
bioRxiv - Genomics 2024Quote: ... 375 U of MboI (NEB, R0147) was used to digest ...
-
bioRxiv - Genomics 2024Quote: ... the DNA was ligated by T4 DNA ligase (NEB # M0202) at 4 °C overnight ...
-
bioRxiv - Genomics 2024Quote: ... 360 μl 10x T4 DNA ligase buffer (NEB, B0202), 15 μl of 400 U/μl T4 DNA ligase (NEB ...
-
bioRxiv - Genomics 2024Quote: ... 10 µL of tagmented DNA was PCR amplified for 14 cycles in a 50 µL reaction volume using NEBNext High-Fidelity 2X PCR Master Mix (NEB # M0541S) and Nextera primers ...
-
bioRxiv - Genomics 2024Quote: ... 75 μl of NEBuffer™ DpnII (NEB, B0543S) and 600U of DpnII (R0543M ...
-
bioRxiv - Genomics 2024Quote: ... 4.5 μl of 10mM dATP (NEB, N0440S), 4.5 μl of 10mM dGTP (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Oligos were annealed and both oligos and vector were digested with AgeI and EcoRI (NEB). Lenti-sgRNA-neo (Addgene #104992 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Second-strand cDNA was synthesized using Deep Vent (exo-) DNA polymerase (NEB) and biotinylated second-strand primer ...
-
bioRxiv - Cancer Biology 2024Quote: ... purified with tRNA-coated streptavidin beads and amplified with 2x Phusion high-fidelity PCR mix (NEB). CAGE libraries were treated with Exonuclease I (NEB ...
-
bioRxiv - Developmental Biology 2024Quote: Tissue samples were then digested with Proteinase K (NEB, P8107S) as described (38 ...
-
bioRxiv - Cell Biology 2024Quote: ... lentivectors for CRISPR Knock-out (CRISPR-KO) experiments via restriction digest of the respective lentivector with BsmBI (NEB, Cat. No. R0739) and T4 ligase (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... and T4 ligase (NEB, Cat. No. M020L). Oligonucleotides (Eurofins Genomics ...
-
bioRxiv - Cell Biology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554), the DpnI (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2024Quote: ... The generated cDNA was used as the template for qPCR analysis by utilizing Universal qPCR Master Mix (BioLabs). At least three independent experiments were performed to indicate the change in gene transcription ...
-
bioRxiv - Cell Biology 2024Quote: ... Double digested PCR product and plasmid were further subjected to Ligation using T4 DNA ligase (NEB) at 16°C for 12-14 hours ...
-
bioRxiv - Synthetic Biology 2024Quote: ... DNA was amplified by PCR in 25 μL reactions using Q5® High-Fidelity DNA Polymerase (NEB). All PCR reactions were set up according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... or Monarch® Plasmid Miniprep Kit (NEB). When purifying plasmid DNA from C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The RNA-DNA hybrids were then digested with thermostable RNase H (NEB, M0523S) at 45°C for 1 hour ...
-
bioRxiv - Zoology 2024Quote: ... RT-qPCR was performed using the Luna One-step RT-qPCR kit (New England Biolabs, Germany) on a 384 well-plate with 3 technical replicates per biological replicate in CFX384 Touch Real-Time PCR (Bio-Rad ...
-
bioRxiv - Zoology 2024Quote: ... between EcoRI and XhoI (NEB, USA) using a pEASY-Basic Seamless Cloning and Assembly Kit (Transgen Bio ...
-
bioRxiv - Zoology 2024Quote: ... and post-aestivation stages) were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module followed by NEBNext kit (E7490, NEB, Ipswich, MA, USA). The quality and quantity of the libraries were checked on an Agilent 2100 Bioanalyzer using the Agilent DNF-935 Reagent Kit (Agilent Technologies ...
-
bioRxiv - Systems Biology 2024Quote: ... The V4-V5 16S variable regions were first amplified in 10 µL reactions in triplicate using the NEB Q5 Hot Start HiFi 2X master mix (New England BioLabs, Ipswich, MA) and 515F and 926R primers as described 59 ...
-
bioRxiv - Genetics 2024Quote: ... IVS5-IVS6) was amplified from genomic DNA isolated from Patient 1 using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with 35 cycles ...
-
bioRxiv - Plant Biology 2024Quote: ... Primers for PCR fragments bearing 20 bp overlapping to the flanking region were produced with Phusion High-Fidelity DNA Polymerase (NEB) and 40-mer primers ...
-
bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the LUNA 1-step RT-qPCR kit (E3005L, NEB) on an AriaMx Real-time PCR system (G8830A ...
-
bioRxiv - Plant Biology 2024Quote: ... 10 μg of gDNA was digested with SacI restriction endonuclease (New England Biolabs). Digested samples were electrophoresed on a 1% agarose gel stained with SYBR Safe (Invitrogen ...
-
Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... and plasmid assembly was performed using NEBuilder HiFi DNA Assembly Mix (NEB), followed by transformation into chemically competent E ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 2.5 U/µL M-MuLV RT (NEB #M0253L) unless indicated otherwise ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A 7 mL fraction of the eluate was mixed with 3 mL of 6x purple gel loading dye (NEB) and loaded in 0.7-1% agarose gel with ethidium bromide ...
-
bioRxiv - Synthetic Biology 2024Quote: ... dephosphorylating the intermediate plasmid fragment with Antarctic Phosphatase (NEB M0289) and ligating with T4 ligase (NEB M0202 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 10 or 20 mM of NH4SO4) (NEB), 0.625 ng/µL of purified DNAP ...
-
bioRxiv - Synthetic Biology 2024Quote: ... where it was mixed with 0.5 units of DNase I (NEB). Reactions were incubated in a nuclease-free PCR tube (VWR ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Inserts were cloned using Golden Gate cloning with BsaI (NEB) into a pET22 plasmid ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Q5U Hot Start High-Fidelity DNA Polymerase (New England Biolabs), or repliQa HiFi ToughMix (Quanta Bio) ...
-
bioRxiv - Synthetic Biology 2024Quote: mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) following the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... all DNA assembly and plasmid propagation was performed in NEB Turbo cells (New England Biolabs) or Mach1F cells 37 (Mach1 T1R cells (Thermo Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... All connections between genes and linearized vectors were achieved by Gibson assembly (Gibson assembly kit, New England BioLabs, UK). The shell-(csoS2-NM) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A fraction (generally 6 µL) of the eluate was mixed with an equal volume of 6x purple gel loading dye (NEB) and loaded in 1% agarose gel with ethidium bromide ...
-
bioRxiv - Synthetic Biology 2024Quote: ... For USER assembly 0.1 to 0.5 pmol fragments were added in an equimolar ratio in a 10 μl reaction that included 0.75 µL USER enzyme (Uracil-DNA Glycosylase and DNA-glycosylase-lyase Endonuclease VIII; New England Biolabs), 1ul rCutSmart Buffer (50 mM potassium acetate ...
-
bioRxiv - Synthetic Biology 2024Quote: ... according to the NEB Electroporation Protocol (#C3020, NEB). Transformants were plated onto LB-Ampicillin 2% agar media in large (150mm ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The Monarch Plasmid Miniprep Protocol (NEB #T1010) was followed from here with no known deviations ...