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Citations for New England Biolabs :
6201 - 6250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Samples were first prepared by restriction enzyme digestion with HaeIII (NEB) for 1 hour at 37 °C followed by ddPCR using a Bio-rad Auto DG droplet generator ...
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bioRxiv - Immunology 2024Quote: were purchased from NEB inc ...
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bioRxiv - Genomics 2024Quote: ... add 8 ul of RNAse A (NEB, 10 mg/ml), and incubate 1 h at 37 C being careful there is no condensation on lid.
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bioRxiv - Genetics 2024Quote: ... Cell pellets were resuspended in 1.2 mL lysis buffer and incubated with 20 μg/mL of lysozyme (NEB) for 30 min on ice ...
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bioRxiv - Genetics 2024Quote: ... 10 mM ribonucleoside vanadyl complex (RVC by NEB), 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: ... These sub-libraries were barcoded and enriched using NEBNext Q5U Master Mix (New England Biolabs, United Kingdom). Sub-libraries were purified using 1.8× AMPureXP and pooled to equimolarity for the final library at ∼20 nM ...
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bioRxiv - Genetics 2024Quote: ... 250 ng of RNA samples were digested at 37°C for 2 hours with Nucleoside Digestion Mix (New England Biolabs, M069S). Digested RNA samples were diluted to 100 µl with double-distilled water and filtered through 0.22 µm Millex Syringe Filters ...
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bioRxiv - Genetics 2024Quote: ... 2 μl of DpnII (NEB) was added to digest the chromatin overnight at 37 °C with shaking at 900 rpm and deactivated at 65 °C for 20 min.
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bioRxiv - Genetics 2024Quote: ... pGL3-U6 vector was linearized by BsaI (NEB) to generate the cloning backbone with 5’ overhangs of ‘TGGC’ and ‘TTTG’ at the two ends ...
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bioRxiv - Genetics 2024Quote: ... the DNA-bound beads were digested with 2 μl BpmI (NEB, catalog number R0565L) for 30 min at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... The proximity ligation was performed with resuspended nuclei by adding 100μl of T4 ligation buffer (NEB) containing 1 μl of T4 ligase and incubated at 16 °C for 24 h ...
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bioRxiv - Genetics 2024Quote: ... Strand synthesized DNA was treated with 40U of Exonuclease I (NEB; M0293S). DNA was purified by SPRI AMPure XP beads (sample to beads ratio 0.8:1 ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA (transfected plasmid DNA) and plasmid DNA samples (that was used for transfection) with Phusion High-Fidelity PCR Master Mix (NEB Catalog no. M0531S) using primer set KC_BC (Table S4 ...
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bioRxiv - Genetics 2024Quote: ... was used for indexing-amplification with NEBNext Multiplex Oligos for Illumina (NEB) by 10-12 PCR cycles ...
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bioRxiv - Genetics 2024Quote: ... sgRNA complementary to the central part of the hACE2 (Table 2) was synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). Library was loaded onto the FLO-MIN106 flow cell and sequenced with GridION (Oxford Nanopore ...
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bioRxiv - Microbiology 2024Quote: ... The pBAD33 plasmid harboring the stem structure of rrsH (corresponding to -131∼-102 and +1559∼+1589 relative to +1 of rrsH gene) under T7 promoter was linearized by digestion with XbaI (NEB, R0145).
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bioRxiv - Genetics 2024Quote: Homology arms were inserted into pBlueSURF (generously provided by Jeff Sekelsky, UNC) by Gibson assembly (NEB). Sequence containing B2 and B3 recombination sites ...
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bioRxiv - Genetics 2024Quote: ... 2 µl Cas9 Protein (M0386, NEB), 0.5µl buffer (NEB) ...
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bioRxiv - Genetics 2024Quote: ... 0.5µl buffer (NEB), up to 5µl with ddH2O
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bioRxiv - Genetics 2024Quote: ... Analysis was done either by amplifying bi-sulfite treated DNA with the Q5U-HotStartHighFidelity Polymerase (NEB) and then analyzing it on a 3% agarose gel or by cloning amplified bi-sulfite treated DNA fragments into the Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing ...
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bioRxiv - Genetics 2024Quote: ... The primers below were used with a Q5 High Fidelity Polymerase (NEB #M0491S) PCR to amplify individual histone array amplicons.
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bioRxiv - Genomics 2024Quote: Strand cDNA Synthesis Kit (New England Biolabs) according to manufacturers recommendations ...
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bioRxiv - Genomics 2024Quote: ... The Chromatin Conformation Capture (Hi-C) library was constructed from enzyme DpnII (NEB, USA) digestion of cross-linked DNA and sequenced to produce 150 bp paired-end reads with an Illumina Novaseq 6000 machine following the manufacturer’s sequencing protocols ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: ... Methylated adaptors were ligated to end-repaired and dA-tailed DNA using the NEBNext Ultra II Ligation Module (New England Biolabs, United Kingdom), followed by USER enzymatic treatment ...
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bioRxiv - Genomics 2024Quote: ... The NEBNext® Ultra II Directional RNA Library Prep kit (New England Biolabs, USA) was used for RNA sequencing library construction ...
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bioRxiv - Genomics 2024Quote: Mix gently and add 5 ul of 20mg/ml proteinase K (NEB) solution to each tube and incubate for 3 hr at 45°C being careful there is no condensation.
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bioRxiv - Genetics 2024Quote: ... a subset of a second identical transformation was taken directly after electroporation and split across ten 1 mL cultures with 10-beta recovery media (NEB) then incubated for 1 hour at 37°C ...
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bioRxiv - Genetics 2024Quote: ... 10μl of DpnII buffer (NEB), and 2.5 μl of 10% SDS and incubated for 1 h at 37 °C with shaking at 900 rpm ...
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bioRxiv - Genetics 2024Quote: ... and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Genetics 2024Quote: ... was generated by amplifying the transgene rgef-1p::PH::miniSOG::unc-54 3’UTR from pNMS03 with primers P37 and P38 containing AvrII restriction sites and cloning the fragment into pCFJ151 after AvrII (New England BioLabs) digestion ...
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bioRxiv - Genetics 2024Quote: ... Using NEBuilder® HiFi DNA Assembly (New England BioLabs), these four amplicons were placed into pCFJ151 (Addgene #19330 ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... Using DNase I (NEB)–treated RNA ...
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bioRxiv - Genomics 2024Quote: ... 40 fmol of plasmid backbone and 2 μL of each annealed oligo pair were added to a one-pot golden gate reaction with Esp3I and T4 DNA Ligase (NEB) as has been done in previous studies73,75 ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: ... then end-prepped and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, United Kingdom). Methylated adaptors were ligated to end-repaired and dA-tailed DNA using the NEBNext Ultra II Ligation Module (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... crRNAs were amplified from genomic DNA (primer sequences can be found in (Supp. Table 2) using Q5 2x Master Mix (NEB) in 100 μL reactions with the following thermal cycling parameters:
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bioRxiv - Genomics 2024Quote: ... cloning was performed using PCR of desired insert fragments with Q5 (New England Biolabs (NEB)) ...
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bioRxiv - Genetics 2024Quote: ... pLVX vector was first linearized by EcoR1 and BamH1 (NEB) and purified as the cloning backbone ...
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bioRxiv - Genomics 2024Quote: ... then phosphorylated using T4 Polynucleotide Kinase (NEB) in a 50 μL reaction ...
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bioRxiv - Genomics 2024Quote: ... while the PCR amplicon was digested with BsaI (NEB). These were purified using the DNA Clean and Concentrator Kit (Zymo) ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Genomic DNA was extracted and purified from 200 μL of whole blood using Monarch Genomic DNA Purification Kits (New England Biolabs, United Kingdom). DNA quantity and quality were assessed using NanoDrop 2000 (Thermo Scientific ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Samples of ∼200 ng genomic DNA were digested with 20 units of MspI (C↓CGG) and 10 units of AluI (AG↓CT) (New England Biolabs, United Kingdom) at 37°C overnight (∼16 hours) ...
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bioRxiv - Genetics 2024Quote: ... a test transformation was performed with 1uL of the 20uL SPRI purified Gibson assembly mixture and 50uL of 10-beta electrocompetent cells (NEB) to determine the optimal amount of Gibson assembly and 10-beta cells to achieve the desired colony-forming unit (CFU ...
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bioRxiv - Genetics 2024Quote: ... with primers (Integrated DNA Technologies, Coralville IA) designed to provide flanking homology arms for HiFi assembly (New England Biolabs, Ipswich MA) into EcoRI/NotI-digested (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and 6 μg of pure enzyme (RlmJ/RlmF) or BSA (NEB, Ipswich, MA, USA) as a control [27] ...
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bioRxiv - Microbiology 2024Quote: ... The RT RNA sample (5 μL) was amplified using the LongAmp Taq 2× Master Mix (NEB, Ipswich, MA, USA) with IVT Nanopore T7 Fw and IVT Nanopore T7 Rv primers (Supplementary Table 5) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was synthesized from a DNA template (Supplementary Table 3) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA) and treated with DNase I (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with Exonuclease I (NEB, Ipswich, MA, USA) for 15 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/µl murine RNase inhibitor (NEB, Ipswich, MA, USA), and 6 μg of pure enzyme (RlmJ/RlmF ...
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bioRxiv - Microbiology 2024Quote: ... quantified the two pools using an NEBNext Library Quant Kit for Illumina (New England Biolabs), and determined average fragment size and adaptor dimer removal on a Tapestation 4200 (Agilent ...