Labshake search
Citations for New England Biolabs :
6151 - 6200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... preincubated at 37°C for 10 min with either a single-guide RNA (sgRNA) generated by in vitro transcription (SP6 RNA Polymerase, New England BioLabs) or hybridized crRNA/tracrRNA (IDT) ...
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bioRxiv - Genetics 2024Quote: ... and subsequently used for transcription by T7 RNA Polymerase (New England BioLabs). Many transcription reactions were pooled and purified using one column to produce concentrated RNA samples ...
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bioRxiv - Genetics 2024Quote: ... PAM sites were abrogated using site-directed mutagenesis (NEB). After experimentally determining that the original guide site in the distal homology arm that we tried did not cut efficiently ...
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bioRxiv - Genetics 2024Quote: ... NEB PaqCI and PaqCI activator (NEB #R0745) were added and thermocycled per manufacturer recommendations ...
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bioRxiv - Genetics 2024Quote: ... The resulting fragment was gel-purified and then used to be transcribed using either MegaScript kits from Ambion or HiScribe kits from NEB according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: Golden Gate Assembly was performed largely according to the manufacturer protocol included with the NEBridge Golden Gate Assembly kit (New England Biolabs) using the with alterations noted below (Potapov et al ...
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bioRxiv - Genetics 2024Quote: ... PCRs were performed according to manufacturer’s protocol (New England Biolabs, M0273) and PCRs were resolved on agarose gels.
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bioRxiv - Genetics 2024Quote: ... PCR samples were combined with 1 μL of 6X gel loading dye (New England Biolabs, Cat. # B7024S) per 5 μL of sample and loaded each the remaining wells ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RT-qPCR was performed with LunaScript RT Supermix (NEB) in a Bio-Rad CFX Opus 96 Real-Time PCR System ...
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bioRxiv - Genomics 2024Quote: ... 1µg RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). RT-qPCR was performed with LunaScript RT Supermix (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The synthesized oligonucleotide pair was phosphorylated and annealed using T4 Polynucleotide Kinase (NEB M0201S) with T4 Ligation Buffer (NEB) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... a fluorescein-conjugated chitin-binding probe (1:200; NEB P5211S) was applied ...
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bioRxiv - Neuroscience 2024Quote: ... we introduced mutations by primers and aligned by HiFi DNA Assembly Master Mix (NEB, E2621L). Ligated PCR products inserted into the pNL1.1 vector (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... All cloning PCR products were amplified by Q5 polymerase (NEB, M049L). For construction of vectors used for luciferase assays ...
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bioRxiv - Neuroscience 2024Quote: ... PCR fragments were ligased by HiFi DNA Assembly Master Mix (NEB, E2621L) and into the pCAG-Puro vector by XhoI enzyme site ...
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bioRxiv - Zoology 2024Quote: ... Proteins were purified from cell lysates using the pMAL protein fusion and purification system (New England Biolabs). The MBP protein was also independently expressed in pMAL-c2x as control ...
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bioRxiv - Systems Biology 2024Quote: ... 500ng poly-A tailed RNA was ligated to ONT RT adaptor (RTA) using T4 DNA ligase (NEB, M0202M) and reverse transcribed by SuperScript III Reverse transcriptase (Invitrogen ...
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bioRxiv - Zoology 2024Quote: ... The transcriptome sequencing libraries were prepared with poly A selection using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and were sequenced using the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Transposed DNA was originally amplified and barcoded in a PCR reaction using NEBnext PCR master mix (NEB, M0544) and 1.25 μM forward and reverse primers originally described in 99 ...
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bioRxiv - Genetics 2024Quote: ... and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621). All regions amplified by PCR were confirmed by DNA sequencing at Genewiz from Azenta Life Sciences (South Plainfield ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Post-capture amplification was performed using NEBNext Q5U Master Mix (New England Biolabs) as follows ...
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bioRxiv - Evolutionary Biology 2024Quote: ... USER treated DNA extracts were purified using Monarch columns (NEB).
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bioRxiv - Genomics 2024Quote: ... and Random Primer 6 (NEB, S1230S). The samples were finally purified using AMPure XP Beads (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... 5α Competent Escherichia coli (NEB) cultured in lysogeny broth was used for plasmid preparation.
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Microbiology 2024Quote: ... Q5 high-fidelity Taq DNA polymerase (New England Biolabs Inc., Ipswich, MA) which has a 3’→ 5’ exonuclease activity and hence results in ultra-low error rates was used for PCR ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). Libraries were sequenced on Illumina NovaSeq 6000 2×150 with the UW-Madison Biotechnology Center at ∼10 million reads per sample.
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bioRxiv - Microbiology 2024Quote: ... DNA was digested with restriction enzymes from New England Biolabs (NEB). PCR products were purified with DNA Spin and Concentrate kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Large (Klenow) Fragment (NEB) for blunting the fragment ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Microbiology 2024Quote: ... Input control and HT-enriched samples were prepared for Illumina sequencing using NEBNext Ultra II Library Prep Kit for Illumina (NEB; E7645S) following manufacturer’s protocol and sequenced on Illumina NovaSeq 6000 2×150 with the UW-Madison Biotechnology Center at ∼10 million reads per sample.
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bioRxiv - Genomics 2024Quote: ... 10% of these RNAs were decapped and polyphosphates reduced to monophosphates using RppH (NEB) to sequence all small RNAs (sRNA-seq).
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bioRxiv - Microbiology 2024Quote: ... and fragments were joined via Gibson Assembly (NEB).
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bioRxiv - Microbiology 2024Quote: ... gRNA oligos from IDT were cloned into Pss013 using Gibson Assembly (NEB). Plasmids were transformed into DH5α E ...
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bioRxiv - Genetics 2024Quote: ... CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; E7645L) and sequenced on NextSeq500 (paired-end ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was digested overnight with MseI in CutSmart buffer (NEB) (1 μg genomic DNA in 30 μl ...
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bioRxiv - Genetics 2024Quote: We amplified the oligo pool using five cycles of PCR with Phusion High-Fidelity polymerase (New England Biolabs) and primer set KC_1 (Table S4) ...
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bioRxiv - Genetics 2024Quote: ... The PCR-amplified oligo pool was then digested with BsaI-HFv2 (NEB #R3733S), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and BstEII-HF (NEB Cat#R3162S), followed by gel purification ...
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bioRxiv - Genetics 2024Quote: ... MSp510_K3-WT was digested with restriction enzymes NotI-HF and MluI-HF (NEB Cat#R3198S), followed by gel purification and size selection for the 8,684 bp band ...
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bioRxiv - Genetics 2024Quote: ... while the K3 alleles were separately digested with BstEII-HF and SacI-HF (NEB Cat#R3156S) to generate a 1,130 bp insert DNA fragment with 20-bp homology arms with homology to the receiver fragment for Gibson Assembly ...
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bioRxiv - Genetics 2024Quote: ... MSp508_MCS was digested with restriction enzymes NotI-HF (NEB Cat#R3189S) and PvuII-HF (NEB Cat#R3151S) ...
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bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
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bioRxiv - Genetics 2024Quote: ... nuclei were lysed and the isolated DNA was digested with CviQI (New England Biolabs, R0639), circularized by ligation and purified ...
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bioRxiv - Genetics 2024Quote: ... The primary restriction enzyme digestion was performed with 800 U NlaIII or ApoI (New England Biolabs, R0125 and R3566). Following in situ ligation of digested chromatin ...
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bioRxiv - Genetics 2024Quote: ... The digested oligos and tevopreq vector were ligated using T4 DNA ligase (NEB #M0202S) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... the vector was titrated via qPCR using Luna SYBR green reagents (New England Biolabs) as per manufacturer recommendations ...