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Citations for New England Biolabs :
6101 - 6150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the H3.1-SNAP and H3.3-SNAP were labeled with SNAP-Cell 505-Star (New England Biolabs, S9103S) for a duration of 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the Monarch Genomic DNA Purification kit (New England Biolabs) was utilized with 1-2 x 106 cells ...
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bioRxiv - Cell Biology 2024Quote: ... StrepII-EPLINα WT plasmid was created by inserting the EPLINα PCR product (created with EPLINα Strep F/R primers) into pcDNA3 StrepII MCS vector cleaved with EcoRI/AgeI using NEBuilder HiFi DNA Assembly (NEB). The deletion mutants (ΔNHX ...
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bioRxiv - Cell Biology 2024Quote: ... DNA libraries were prepared according to the manufacturer’s protocol (NEBNext® Ultra II, NEB). Sequencing was performed on the NovaSeq Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... the single stranded gRNA oligos were annealed in NEBuffer 2 (New England Biolabs) at 95 °C for 5 min and left to cool ...
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bioRxiv - Cell Biology 2024Quote: ... Reactions (10 µl) were performed Luna Universal qPCR Master Mix (New England Biolabs). mRNA abundance for each gene was determined relative to GAPDH mRNA using the 2−ΔΔCt method ...
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bioRxiv - Cell Biology 2024Quote: ... and reversely transcribed into cDNA using the ProtoScript II NEB cDNA synthesis kit (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... Nuclear extraction buffer (NEB) was added to the pellet ...
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bioRxiv - Cell Biology 2024Quote: ... for 20 min at 37 °C in RNAse H buffer (NEB). To image and segment whole l.2 Mb tracing loci ...
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bioRxiv - Cell Biology 2024Quote: ... and BamHI-HF (NEB R3136) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In vitro transcription was carried out with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), followed by the template DNA digestion with DNase I (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells transfected with Apaf1-SNAP-tag were stained with 1.5 nM SNAP-Cell 647-SiR substrate (NEB, S9102S), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Purple (6X) (NEB, Cat No. B7024S). The bands were developed and imaged using a UVITEC Gel Documentation System (Cleaver Scientific).
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of RNA were retrotranscribed into cDNA using a LunaScript™ RT SuperMix cDNA Synthesis Kit (NEB, Cat. No. E3010L) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum in placenta samples was evaluated using One Taq® Quick-Load® 2X Master Mix with Standard Buffer (NEB, Cat No. M0486L) and the following thermocycler program ...
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bioRxiv - Cell Biology 2024Quote: ... rRNA-depleted RNA was used to generate the RNA-sequencing libraries using NEB Ultra II directional kit (New England Biolabs). Samples from Keratinocytes were multiplexed and sequenced on a HiSeq4000 PE 150 sequencing platform (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time quantititative PCR (qRT-PCR) detection was performed using the Luna® Universal qPCR Master Mix (M3003, New England Biolabs) and the CFX96 Real-Time PCR System ...
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bioRxiv - Cell Biology 2024Quote: ... We performed the SNAP-tag labeling in vivo with 2 μM SNAP-Cell TMR-Star (New England Biolabs) and visualized histones incorporated into chromatin after a pre-extraction of soluble histones before fixation with 2% paraformaldehyde for 20’ as 32 ...
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...
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bioRxiv - Cell Biology 2024Quote: ... Each library was uniquely indexed with NEBNext Multiplex Oligos for Illumina (NEB E6440S), then pooled together in equimolar amounts ...
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bioRxiv - Cell Biology 2024Quote: ... a total of 600 ng of genomic DNA from the relevant cell lines was fragmented using DNA fragmentase (NEB, M0348) for 25 minutes at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... coli (NEB C3019) grown on LB Agar plates with 100ug/mL ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England BioLabs) and eluted in RNAse-free water ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra TMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced with an Illumina NovaSeq 6000 to obtain 150 bp ×2 paired-end reads ...
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bioRxiv - Cell Biology 2024Quote: ... AcGFP and TagRFP-657 proteins and SNAP-Surface 488 and Alexa 647 dyes (New England Biolabs) were measured in the same excitation and emission ranges as in flow cytometry assays ...
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bioRxiv - Cell Biology 2024Quote: ... infrared IR800-dye conjugated 3’ adapters were ligated overnight using RNA ligase 1 (NEB) in a high-percentage PEG8000 buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and the RNAs were 3’end dephosphorylated by PNK (NEB) and FastAP phosphatases (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... These cells that were pre-stained with 5 µM SNAP-Surface 647 (New England Biolabs) for one hour at room temperature if necessary were stained with 5µg/mL of Alexa488 labeled lectins (PNA ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM Ribonucleoside vanadyl complex (RVC, New England Biolabs), and 40U/ml RNAsin plus (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson reactions were set up with NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), 50ng of linearized backbone ...
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bioRxiv - Cell Biology 2024Quote: ... Exonuclease I (0.15 U/µl, New England Biolabs) and exonuclease III (0.77 U/µl ...
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bioRxiv - Cell Biology 2024Quote: ... Components of the insert were PCR amplified using Q5-Hot Start DNA Polymerase (NEB M0493) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... according to the manufacturer’s instructions and then treated with Antarctic Phosphatase (New England Biolabs) and incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: EGFP was PCR-amplified from pCIneo-FlucDM-EGFP119 and subcloned in pcDNA4/TO using the restriction enzymes XhoI and XbaI (NEB). Nudfa1 and Ndufa2 was amplified from HEK293T cDNA and cloned into pcDNA4/TO-EGFP using KpnI and XhoI (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... and exonuclease III (0.77 U/µl, New England Biolabs) were then added with NEBuffer I to digest DNA with exposed end ...
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bioRxiv - Cell Biology 2024Quote: ... Nudfa1 and Ndufa2 was amplified from HEK293T cDNA and cloned into pcDNA4/TO-EGFP using KpnI and XhoI (NEB). Gene sequences of At-A1 ...
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bioRxiv - Cell Biology 2024Quote: ... the entry vectors pENTR2B-EPLINα and pENTR2B-EPLINβ were first generated using HiFi DNA assembly according to the manufacturer’s instructions (New England Biolabs).The primer sequences used to make the plasmids are shown below ...
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bioRxiv - Cell Biology 2024Quote: ... pBluescript II KS (+) and ORF were digested using SacI and SalI (New England Biolabs) restriction enzymes according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was performed using Luna Universal qPCR Master Mix (NEB, M3003L) and a Roche LightCycler 96 machine (Roche) ...
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bioRxiv - Genetics 2024Quote: ... Phosphothreonine (New England Biolabs, 9386S), PCNA (Abcam ...
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bioRxiv - Genetics 2024Quote: ... and 400 units of Lambda protein phosphatase (λpp) (New England Biolabs, P0753S). The reactions were incubated at 30°C for 30 minutes and terminated by an equal volume of 2x Laemmli buffer.
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bioRxiv - Genetics 2024Quote: ... amplified from pCZGY2851 (gift from Andrew Chisholm) with primers P33 and P34 using NEBuilder® HiFi DNA Assembly (New England BioLabs). pNMS03 (40 ng/μl ...
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bioRxiv - Genetics 2024Quote: ... with SD20414 and SD20415 for pacs-1 or SD20416 and SD20417 for wdr-37 (Table S2) using Phusion HF DNA Polymerase (NEB) and cloned into pCR8 by TOPO-TA cloning (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... digested with AflII (New England BioLabs) and treated with CIP (New England BioLabs ...
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bioRxiv - Genetics 2024Quote: ... The sequence-verified ORFs were cloned using GIBSON (NEB, #E2611L) into the pLV-EGFP plasmid ...
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bioRxiv - Genetics 2024Quote: ... Libraries were electroporated into NEB5α electrocompetent cells (NEB), grown overnight and plasmid DNA extracted using Qiagen Maxi Prep ...
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bioRxiv - Genetics 2024Quote: ... and alkaline phosphatase (NEB, M0525S) with additional incubation for 2 hours as before ...