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Citations for New England Biolabs :
6001 - 6050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... USER treated DNA extracts were purified using Monarch columns (NEB).
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bioRxiv - Immunology 2024Quote: ... 100 ng of the WTA library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Purified adapter-ligated products were subjected to index PCR ...
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bioRxiv - Plant Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... and 8 U RNase Inhibitor Murine (New England Biolabs). The reaction was incubated at 50°C for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA was amplified using Q5 Polymerase (New England Biolabs) with an initial denaturation at 98°C for 30 seconds ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting cDNA and qDNA were amplified with Q5 Polymerase (New England Biolabs), and the gel-purified PCR products were sequenced.
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bioRxiv - Plant Biology 2024Quote: ... DNase I-treated total RNA from Arabidopsis was used for reverse transcription with Induro (New England Biolabs) and random hexamers ...
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bioRxiv - Plant Biology 2024Quote: Reverse transcription was performed with Induro (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting amplicons were cloned into the pMiniT2.0 vector (New England Biolabs), and plasmid DNA from 18 colonies was purified and Sanger sequenced ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µg of total RNA was circularised in a reaction containing 6 U T4 RNA Ligase 1 (New England Biolabs), 50 µM ATP ...
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bioRxiv - Neuroscience 2024Quote: Constructs were all generated using the Gibson Assembly Cloning Kit (New England Biolabs) by inserting the different DNA fragments (PCR-generated or gBlocks ...
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bioRxiv - Genomics 2024Quote: ... samples were first treated with Antarctic Phosphatase (NEB), followed by treatment with T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A library was then prepared following the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit procedure (New England Biolabs, Ipswich, MA, USA). A library was directly prepared from 100 ng of total RNA extracted from floral buds using the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Gibson assemblies were transformed the assembly mix into DH5α competent cells (NEB #C2987H). Plasmid backbones were digested with restriction enzymes and purified using Qiagen QIAquick gel extraction kit (#28706) ...
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bioRxiv - Neuroscience 2024Quote: ... and were subsequently incubated with the respective restriction enzyme (BioLabs) for 1.5 h at the specified temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Escherichia coli and Myxcococcus xanthus were amplified from genomic DNA by PCR (Q5® High-Fidelity 2X Master Mix, New England Biolabs, USA-MA) and introduced into the pLIC expression vector55 by Gibson cloning (Gibson Assembly Master Mix ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and introduced into the pLIC expression vector55 by Gibson cloning (Gibson Assembly Master Mix, New England Biolabs, USA-MA). All other extant and ancestral CS sequences were obtained as gene fragments from Twist Bioscience (USA-CA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... For single-site mutants and deletions of the CS-sequences the KLD Enzyme Mix (New England Biolabs, US-MA) was used ...
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bioRxiv - Genetics 2024Quote: ... Ribonucleoprotein complexes of SpCas9 protein (New England Biolabs) and gRNAs were prepared with a final concentration of 200 ng/μl and 25 ng/μl for each gRNA (100 ng/μl in total) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We also performed a fifth extraction with spleen tissue using Monarch® HMW DNA Kit (New England BioLabs) for Ultra-Long library preparation.
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bioRxiv - Genetics 2024Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit (New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and size selected before library preparation using the NEB DNA Ultra II (New England Biolabs), following Arima’s protocol ...
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bioRxiv - Genetics 2024Quote: ... after extracting total RNA from the testis (three replicates per genotypes) using the Monarch total RNA mini prep Kit (New England Biolabs), we generated RNA-seq libraries using the NEBNext Rrna Depletion Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... and domain deletion mutants were made using site directed mutagenesis (NEBasechanger or NEBuilder (New England Biolabs (MA)) ...
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bioRxiv - Genetics 2024Quote: ... We treated DNA extracts with USER (NEB) during library preparation to cut DNA at uracils ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli dam-/dcm- was purchased from New England BioLabs (NEB) and used for de-methylation of plasmid DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting ligated products were used to transform 50 uL of electrocompetent cells (NEB 10Beta ...
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bioRxiv - Synthetic Biology 2024Quote: ... The New England Biosciences (NEB) Golden Gate Assembly Kit (BsaI-HF v2 ...
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bioRxiv - Synthetic Biology 2024Quote: RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation ...
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bioRxiv - Evolutionary Biology 2024Quote: The RNA prepared as described above was sent to Macrogen Japan for library preparation and cDNA sequencing as follows: ribosomal RNA (rRNA) was removed from each sample using the NEBNext Bacteria rRNA Depletion Kit (New England BioLabs, Ipswich, MA), and library preparation was performed using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... or Phusion® High-Fidelity DNA Polymerase (New England Biolabs®) were used for reactions where high fidelity PCR amplification was required ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... UDG-treated DNA libraries were re-amplified with NEBNext Q5 Master Mix (NEB) and P5 and P7 primers to a concentration of approximately 30 ng/µL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 µl 1,000 U/µl USER Enzyme (NEB), Ultrapure water (Thermo ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The concentrate was combined with 10x modified Qiagen PB buffer as described in (57) and purified using Monarch columns (NEB). After DNA binding to the Monarch columns ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... Approximately 1 kb upstream and downstream of speEB were amplified and assembled via ITA into pMAD digested with BglII and EcoRI (New England Biolabs, R0144 and R3101, respectively), resulting in plasmid pNOBb41 (Table 4) ...
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bioRxiv - Genetics 2024Quote: ... DNA regions for plasmid assembly were identified using Biocyc (41) and amplified by touchdown PCR from the bacterial chromosomes using Q5 DNA polymerase (New England Biolabs, M0492) and oligonucleotides from Integrated DNA Technologies (Coralville ...
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bioRxiv - Genetics 2024Quote: ... An amplicon DNA library was made by designing amplicons to the coding regions of NR1H3 and PCR was undertaken using Q5® Hot Start High-Fidelity 2X Master Mix (Catalogue number M0494S, New England Biolabs® Inc) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... These amplicons were assembled in pDG1664 digested with EcoRI and HindIII (New England Biolabs, R3101 and R3104, respectively) by ITA and were expressed under the control of their respective native promoters ...
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bioRxiv - Neuroscience 2024Quote: The MERSCOPE 500 gene imaging kit was activated by adding 250 μl of Imaging Buffer Activator (Vizgen, #203000015) and 100 μl of RNAse Inhibitor (New England BioLabs, M0314L). 15 ml of mineral oil was overlaid on top of the imaging buffer through the activation port ...
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bioRxiv - Biochemistry 2024Quote: ... Methylated template DNA was digested by DpnI (NEB) and 5 µl of the reaction were directly transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... and ligation with T4 DNA Ligase (NEB). Approx ...
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bioRxiv - Biochemistry 2024Quote: ... PCRs were performed with 2xQ5 HotStart MasterMix (NEB). Plasmids were generated by Q5 Site-Directed Mutagenesis or Gibson Assembly (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was PCR amplified using the primers directed against 5′ and 3′ RNA cassettes and NEB Q5 HotStart polymerase (NEB). To introduce unique barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL Dnp1 restriction enzyme (NEB, #R0176S) was added to the PCR mixture and the sample incubated at 37 °C for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... coli T7 Express lysY (New England Biolabs) were first grown overnight in 10 mL of lysogeny broth (LB ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...