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Citations for New England Biolabs :
5951 - 6000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and phosphorylated according to the following protocol: 20 nmol of the pooled probes were phosphorylated in 1X PNK buffer (B0201S, New England Biolabs), 1 mM ATP (P0756S ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mM ATP (P0756S, New England Biolabs) and 20 units of T4 Polynucleotide kinase (M0201L ...
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bioRxiv - Developmental Biology 2024Quote: ... and 20 units of T4 Polynucleotide kinase (M0201L, New England Biolabs), in a total volume of 50 uL ...
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bioRxiv - Immunology 2024Quote: ... aegypti embryos were injected with a microinjection mix containing 402.5 ng/µL SpCas9 protein (New England Biolabs), 40 ng/µL of each of three sgRNAs (x1_30rev ...
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bioRxiv - Genetics 2024Quote: ... with constructs cloned in place of GFP via the Gibson assembly method using the NEBuilder HiFi Cloning Kit (NEB, #E5520S). GPS lentiviral vectors encoding the N-terminus of PTGS1 and the C-terminus of TNNC2 fused to GFP were gifts from Stephen Elledge ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription was performed with 1 µg of RNA using one-step reaction using LunaScript RT SuperMix Kit (NEB, #E3010) as indicated by the manufacturer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 250 ng of DNA was digested with the restriction enzymes PstI (recognising the rare CTGCA/G motif) and MspI (recognising the frequent motif C/CGG; both enzymes from New England Biolabs, Ipswich, MA, USA). After the ligation of two adapters P1 and P2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All plasmids were cloned into chemically competent NEB-5α or NEBStabl (NEB). Plasmids were purified using PureYield miniprep kits and quantified by NanoDrop A260 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All other variant libraries were synthesized as oligo pools (NEB) covering the TRIM5α sequence between the engineered internal XhoI site and the flanking EcoRI site.
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bioRxiv - Evolutionary Biology 2024Quote: Oligo pools were converted to double-stranded DNA by reverse-strand synthesis using Q5 polymerase (NEB) and the reverse primer only (human ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted high molecular weight DNA from brain and liver tissue with the NEB Monarch HMW DNA Extraction Kit for Tissue (Catalog #T3060S, NEB, Ipswich, Massachusetts). The purified DNA was size selected for >40kb fragments with the PacBio SRE XL buffer (Catalog #102-208-400 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by DpnI digestion and blunt end ligation using KLD enzyme mix (NEB). Sabaeus TRIM5α (XM_008019878.2) ...
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bioRxiv - Developmental Biology 2024Quote: ... along with 5 ng/μl Pmyo-3::GFP and 20 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate the DLS344 strain ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated mRNA (polyA mRNA) was selected with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs [NEB], cat# E7490L) and polyA mRNA sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... Ribosomal RNA was depleted from total input RNA using the NEBNext rRNA Depletion Kit (NEB cat. #E6310). First and second strand synthesis ...
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bioRxiv - Genomics 2024Quote: ... 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each HDI-STARR-seq-treated mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction then purified by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). The purified RNA was digested with Turbo DNase-I (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... and used for Illumina library using the NEBNext ultra II kit (NEB E7600). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate the DLS327 and DLS330 strains ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB #T2010S). The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit ...
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bioRxiv - Immunology 2024Quote: ... and T4 ligase (NEB), transformed into Stellar competent cells (Clontech ...
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bioRxiv - Immunology 2024Quote: ... Donor and destination plasmids were combined in a single digestion-ligation reaction to generate an expression plasmid encoding the heavy and light chains with BsaI-HF (NEB) and T4 ligase (NEB) ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with λ- phosphatase (P0753S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... per manufacturer’s instructions and coupled to streptavidin beads (NEB). Patient plasma (diluted 1:5 in 0.5M NaCl ...
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bioRxiv - Genomics 2024Quote: ... The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB) with NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genomics 2024Quote: ... The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB). The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Library preparation was performed by EMBL Genomics Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L), in combination with the NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were quantitated using NEBNext Library Quant Kit (NEB, E7630,). For RNA sequencing ...
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bioRxiv - Genomics 2024Quote: ... and dTTP and incubated with 20 units of Klenow fragment (NEB) at 37°C for 45 minutes ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The RNA samples were cleaned with Monarch RNA Cleanup Kit (NEB #T2030) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and library amplification using Phusion HF PCR kit (NEB #E0553L).
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... in combination with the NEBNext Poly(A) mRNA Magnetic Isolation Module for mRNA enrichment (NEB #E7490L), according to the manufacturer’s instructions ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... RNA was then extracted by adding an equal volume of RNA lysis buffer and proceeding with Monarch Total RNA Miniprep Kit (NEB #T2010S) extraction ...
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bioRxiv - Genomics 2024Quote: ... RNA was then quantified by nanodrop and ∼500 ng of RNA was used as input for cDNA synthesis using dT priming with Protoscript II Reverse transcriptase (NEB, M0368S). cDNA was then further diluted 2-fold in water and used as input for qPCR as described above ...
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bioRxiv - Genomics 2024Quote: ... The assembly was then purified with the Monarch PCR & DNA cleanup kit (NEB, T1030S), eluted in 6 µL of water ...
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bioRxiv - Genomics 2024Quote: ... with a final extension of 72 °C for 2 minutes using Q5 Hot Start High-Fidelity DNA Polymerase (NEB M0493L). We used 7 cycles for all amplicons ...
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bioRxiv - Genomics 2024Quote: ... digested using 50U/ul DpnII (NEB # R0543M) at 370C overnight ...
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bioRxiv - Genomics 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (NEB). The libraries were sequenced by the NextSeq 500 ...
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bioRxiv - Genomics 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (NEB). The libraries were sequenced by the NextSeq 500 ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... with or w/o 500 µM auxin and the same volume of 2x Monarch DNA/RNA Protection Reagent (NEB #T2011-1) was added before snap-freezing and storage at −80°C until RNA extraction.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II Directional kit(NEB, Cat no E7760L) with ribodepletion (Qiagen QIAseq FastSelect -rRNA HMR Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA-Seq libraries were prepared using the NEBNext DNA library preparation kit according to the manufacturer’s instructions (E6040, New England Biolabs) and the libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Physiology 2024Quote: The cDNA for WT and mutant CFTR in pGEMHE was linearized (Nhe I, New England Biolabs), transcribed in vitro (mMESSAGE mMACHINE T7 Transcription Kit ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB).
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bioRxiv - Systems Biology 2024Quote: ... PCR also introduced specific i5 (NEB, 7780) and N8 (Vazyme ...
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bioRxiv - Systems Biology 2024Quote: ... HiScribe® SP6 RNA Synthesis Kit (NEB) was used to in-vitro transcribe a library of mRNA reporters from linearized plasmids ...
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bioRxiv - Systems Biology 2024Quote: ... The material was made into blunt-end dsRNA products with 15U T4 DNA polymerase (NEB, USA) for 20 minutes at 16°C and reactions stopped by addition of 20mM EDTA (pH 8.0 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligodeoxynucleotides (Table S2) for detection of specific RNA species were end-labeled with [γ- 32P]-ATP using T4 polynucleotide kinase (PNK) (NEB). Prehybridization and hybridization were performed in Church buffer [0.5 M phosphate buffer (pH 7.2) ...