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Citations for New England Biolabs :
5901 - 5950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Gibson assembly reactions were carried out with the NEBuilder Hifi DNA Assembly Master Mix (E2621, NEB) following supplier instructions ...
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bioRxiv - Biophysics 2024Quote: ... coli cells (NEB) and individual colonies were used to inoculate 50 mL overnight LB cultures grown at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... coli 10-beta cells (NEB). Instead ...
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bioRxiv - Biochemistry 2024Quote: 1μl of total cellular RNA was used for sequencing library generation by NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2024Quote: ... Then 3 µL USER Enzyme (NEB, USA) was used with size selected ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were transformed into BL21(DE3) (NEB) and 30 mL of single-colony culture was grown in Terrific Broth II (MP Biomedical ...
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bioRxiv - Biochemistry 2024Quote: ... Purified RNA was reverse transcribed with Luna Superscript Mix (NEB). RNA transcripts of viral and host genes were measured by quantitative PCR using primers listed in Table 2 and Power SYBR Green kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) (New England Biolabs). Protein expression was induced with 0.1 mM IPTG and grown overnight at 20°C ...
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bioRxiv - Biochemistry 2024Quote: ... Synthesized cDNA was then diluted 4-fold and 1 μL was used as template for real-time qPCR using Luna Master Mix (NEB). Values were normalized to GAPDH ...
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bioRxiv - Biochemistry 2024Quote: Oligonucleotides were 32P end radiolabeled using T4-polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... gRNA targeting a 20 nt genomic region adjacent to a Cas9 protospacer adjacent motif (PAM) was first annealed and cloned into BsmbI (NEB) digested pLentiCRISPRv2 vector ...
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bioRxiv - Biochemistry 2024Quote: Illumina libraries were prepared using NEBNext Ultra DNA II Library Preparation Kit (New England Biolabs, Ipswich, MA, USA). 500 ng of DNA was fragmented using a Covaris E220 ...
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bioRxiv - Biochemistry 2024Quote: ... 8 units of T7 exonuclease (NEB) were added ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting total RNA was treated with DNase I (NEB, M0303L) to avoid DNA contamination.
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bioRxiv - Biochemistry 2024Quote: ... Linear plasmid-based substrates were generated by digestion with HindIII restriction enzyme (NEB) according to the manufacturer’s instructions followed by heat inactivation ...
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bioRxiv - Neuroscience 2024Quote: ... The following PCR mixture was prepared for both preliminary and preparative PCR: Hot Start Taq DNA polymerase (2.75 U, New England Biolabs), 1X Hot Start Taq reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... coli DNA Polymerase I (NEB #M0209L). For Nick translation the nicking endonuclease Nt.CviPII was excluded ...
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bioRxiv - Neuroscience 2024Quote: ... NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544). The resulting PCR products were purified through double-sided AMPure XP beads cleanup (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was amplified using the Taq DNA polymerase with ThermoPol®-Buffer (New England Biolabs, Ipswich, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB, E2611L) at 50 °C for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, E7420L). Library quality was assessed using Agilent 2100 bioanalyzer (High Sensitivity DNA Kit) ...
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bioRxiv - Molecular Biology 2024Quote: ... poly(A) selection was performed using Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) with 500 ng of total RNA as input ...
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bioRxiv - Biochemistry 2024Quote: ... and indexed primers (NEB). All libraries were purified on a 3% low melting point agarose gel.
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bioRxiv - Biochemistry 2024Quote: ... 2 µL 10× T4 RNA Ligase Reaction Buffer (NEB), 6 µL PEG8000 (50%) ...
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bioRxiv - Biochemistry 2024Quote: ... which was performed with the NEBNext Universal PCR Primer for Illumina (NEB) and indexed primers (NEB) ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5 μL Rnl1 (high conc) ligase (NEB, M0437M) were added the ligation mixture was incubated at 25 °C for 12 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting DNA molecules were ligated into a pET28a vector (kanamycin resistance) with Instant Sticky-end Ligase Master Mix (NEB) and transformed for amplification in DH5α E ...
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bioRxiv - Biochemistry 2024Quote: ... was added into each ligation mixture by incubation at 30 °C for 1hour followed by adding 1 µL RecJf (NEB, M0264L) for ssDNA digestion at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... or IMPa (NEB, P0761). For EndoH and EndoF2-treated samples ...
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bioRxiv - Biochemistry 2024Quote: ... or EndoH (NEB, P0702S) and EndoF2 (NEB ...
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bioRxiv - Biochemistry 2024Quote: 27.4 μg of total RNA was fragmented into ∼150-200 nt fragments at 94 °C for 5 minutes using the magnesium RNA fragmentation buffer (NEB, E6186AVIAL), followed by purification with OCC ...
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bioRxiv - Biochemistry 2024Quote: The library was prepared following our previously reported procedure with slight changes.[21] 3′-End repair and 5′-phosphorylation were conducted with T4 polynucleotide kinase (PNK) (NEB, M0201S). 16 µL RNA was mixed with 2 µL 10× T4 PNK reaction buffer and 1 µL T4 PNK ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL of 20 mg/mL Proteinase K (NEB), and 1 μL of 10 mg/mL RNase A (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: Cy3-labeled enhancer-promoter DNAs used for condensation assay were generated by PCR using Taq polymerase (NEB) with a Cy3-labeled primer (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
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bioRxiv - Biochemistry 2024Quote: ... 400 ng of the purified mRNA was decapped with 20 units of RppH (NEB, #M0356) in 1× Thermopol buffer (TritonX-100 concentration was reduced to 0.01% ...
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bioRxiv - Biochemistry 2024Quote: ... and then 5’ phosphates of the nucleotide sample were removed with 0.5 units of quick-CIP (NEB, #M0525) at 37 ºC for 2 hr with agitation at 800 rpm for 1 min every 10 min.
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... Decapped mRNA was digested to single nucleotides with 50 units Nuclease P1 (NEB, #M0660) in 20 mM NH4Ac at 37 ºC for 12 hr ...
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bioRxiv - Biochemistry 2024Quote: ... MBP-PR18, MBP-TEV-FUS, and MBP-hnRNPA2 (NLS, residues 298‒336) were purified using affinity chromatography (Amylose resin, NEB), and MBP-PR18 was further purified using cation exchange chromatography (HiTrap SP HP ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were cleaned using a 50 μg Monarch RNA Cleanup Kit (New England Biolabs) and treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Lec5 and Lec9 CHO cells was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050L). Resulting gDNA was then sheared by passing each sample 25 times through a 26G needle ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was then amplified in the first round of PCR using Q5 DNA Polymerase (NEB), and Illumina barcodes were appended during a second PCR ...
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bioRxiv - Bioengineering 2024Quote: ... Ligations were prepared using T4 RNA Ligase I (New England Biolabs, Cat# M0204) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... were then cloned by HiFi DNA Assembly (New England Biolabs) into the pLew100v5-HYG expression vector (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... Tag variants were generated by ligating synthetic dsDNA oligos (IDT) encoding each respective tag downstream of GFP bewteen BamHI and HindIII sites (NEB). In order to generate the GFP-VapCYALAA fusion ...
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bioRxiv - Biochemistry 2024Quote: ... and T4 DNA ligase (NEB). GFP variants were cloned downstream of a 7xHis-SUMO tag in a modified pET22b vector (EMD Millipore) ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no ...