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Citations for New England Biolabs :
5551 - 5600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... coli (NEB), and the complete sequence was confirmed by Nanopore sequencing (Plasmidsaurus).
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bioRxiv - Microbiology 2024Quote: ... mutagenic primers were designed using the New England Biolabs online software NEBaseChanger (primer sequences are available at doi: XXXX. The Q5 Site-Directed Mutagenesis Kit (New England BioLabs) was then used with the A/Baltimore/R0675/2019 hemagglutinin plasmid to produce plasmids with the desired mutations ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s instructions (New England BioLabs / Oxford Nanopore Technologies). 50ng of the barcoded library was sequenced using the R9.4.1 flow cell on a GridION (Oxford Nanopore Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... Individual segments were amplified from cDNA with Phusion High-Fidelity DNA Polymerase (New England BioLabs) using segment-specific primers ...
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bioRxiv - Microbiology 2024Quote: ... was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl) (NEB #B6004S) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were then ligated with T4 ligase into pUC19 vector that had been linearized with BamHI and dephosphorylated with shrimp alkaline phosphatase (rSAP; NEB). The resulting plasmids were transformed into Stellar Competent E ...
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bioRxiv - Microbiology 2024Quote: ... N-4005, and N-4004) were used to cap available 3’-terminal ends using terminal transferase (1 µl, 20 units) (NEB #M0315S), TdT reaction buffer (1.5 µl) ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were mixed with Hydrophilic Streptavidin Magnetic Beads (5 µl) (NEB #S1421S) and binding buffer (500 µl ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: ... A customized linker (rand10_3Tr3) was added to the 3’ end of on-bead LAM products using T4 RNA ligase I (M0204S, NEB). Nested PCR was performed using indicated primer set (3Tr3-Adaptor_R and JM_F ...
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bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The UNC13A CE was amplified with a forward primer in exon 19 5’-CAGACGATCATTGAGGTGCG-3’and reverse primer in exon 22 5’-ATACTTGGAGGAGAGGCAGG-3’using Q5 High Fidelity Master Mix (NEB). PCR products were resolved on a TapeStation 4200 (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs). RNA quality was confirmed after each purification with a TapeStation 4150 (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was stopped with ribonucleoside vanadyl complex (NEB, Cat. No. S1402S) at a final concentration of 20 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl Proteinase K (NEB, NEB, P8107S), 5 µl H2O (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... primer O3P (Supplementary Table 3) was attached to IP-purified DNA and was extended by NEBNext Ultra II Q5 Master Mix (New England Biolabs), followed by ExoI (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... and RNA was isolated using a commercially available kit (Monarch Total RNA Miniprep Kit; New England Biolabs Inc., cat# T2010S).
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bioRxiv - Neuroscience 2024Quote: ... Tissues were homogenized in 1x DNA/RNA Protection reagent (New England Biolabs Inc., Part: T2011-1) and RNA was isolated using a commercially available kit (Monarch Total RNA Miniprep Kit ...
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bioRxiv - Neuroscience 2024Quote: ... exon 7 of PSEN1 was amplified and screened by restriction digest by EcoR V (New England Biolabs). Sanger sequencing was used to confirm the mutation (Azenta) ...
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bioRxiv - Microbiology 2024Quote: ... 1µg of total RNA was converted into cDNA with Protoscript II reverse transcriptase (NEB). The PKR open reading frame was amplified with primers PKR-exon2-F and PKR-exon17-R ...
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10-beta (NEB # C3019H). For vectors containing the ccdB/CmR cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced on an Illumina NextSeq 500 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: Level 0 entry vectors were created through a scaled down NEBuilder (NEB, Ipswich, MA) reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621S), NEBridge Ligase Master Mix (NEB # M1100S) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
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bioRxiv - Molecular Biology 2024Quote: All consumable enzymes for restriction ligation and Gibson assembly were procured from NEB (New England Biolabs, Ipswich, MA) including BsaI-HF-v2 (NEB #R3733S) ...
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bioRxiv - Molecular Biology 2024Quote: ... SEGS-1 region N was generated by cleaving the Kpn1 site using the Q5-site directed mutagenesis protocol (New England Biolabs, Ipswich, MA) from S1-1.5a (pNSB1829 ...
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start Taq DNA Polymerase kit (New England Biolabs) was used according to the manufacturer’s protocol to amplify the SEGS-1 region from DNA samples collected from 4 plants of each genotype ...
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bioRxiv - Molecular Biology 2024Quote: ... They were synthesized by an in vitro reaction with T7 RNA polymerase (New England Biolabs) as described previously 24 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed three times with buffer B (1.2 M sorbitol and 100 mM potassium phosphate buffer pH 7.5) and resuspended in 500 μL of spheroplast buffer (buffer B containing 20 mM VRC (Ribonucleoside–vanadyl complex NEB #S1402S), and 25 U of Lyticase enzyme (Sigma #L2524 ...
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bioRxiv - Molecular Biology 2024Quote: ... which was then used to construct the IP library following the protocol (NEB, USA). The input library was constructed without the antibody treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBridge Ligase Master Mix (NEB # M1100S), and cloning strains of E ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified fragment was added to pWM530 digested with the same enzymes and ligated using T4 DNA ligase (NEB) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were constructed using NEBuilder HiFi DNA Assembly or USER cloning (New England Biolabs) and purified with the Zyppy Plasmid Miniprep Kit (Zymo).
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bioRxiv - Molecular Biology 2024Quote: ... Chromatinized DNA (100 ng) was digested using MNase (NEB) in 25 µL reactions with 2.5–25 units of enzymes that was incubated for 5 minutes at 37 °C before 1 mM of CaCl2 was added and incubation continued for another 5 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL of CutSmart Buffer (NEB #B6004S) for one hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid was then digested with EcoRI and EcoRV (NEB) overnight to generate linear chromatin templates (i.e. ...
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bioRxiv - Molecular Biology 2024Quote: ... including DH5α (NEB #C2987H) and 10-beta (NEB # C3019H) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.25 U of Hotstart Taq Polymerase (New England Biolabs), 10 µM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and antarctic phosphatase (NEB) in the presence of 1mM MnCl2 for 1h on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by overnight treatment with apyrase (1-unit, NEB) on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Apyrase (1-unit, NEB) was then added ...
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bioRxiv - Molecular Biology 2024Quote: ... Apyrase (1 unit, NEB) was added for an additional 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... and BamHI-HF (NEB R3136L). Three variants of the H3 gene with HA tag ...
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bioRxiv - Molecular Biology 2024Quote: ... The P8 and P3 phagemids were double-digested using NcoI-HF® (NEB) and XhoI (NEB) ...