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Citations for New England Biolabs :
5801 - 5850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2 µL 10× T4 RNA Ligase Reaction Buffer (NEB), 6 µL PEG8000 (50%) ...
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bioRxiv - Biochemistry 2024Quote: ... which was performed with the NEBNext Universal PCR Primer for Illumina (NEB) and indexed primers (NEB) ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5 μL Rnl1 (high conc) ligase (NEB, M0437M) were added the ligation mixture was incubated at 25 °C for 12 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting DNA molecules were ligated into a pET28a vector (kanamycin resistance) with Instant Sticky-end Ligase Master Mix (NEB) and transformed for amplification in DH5α E ...
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bioRxiv - Biochemistry 2024Quote: ... was added into each ligation mixture by incubation at 30 °C for 1hour followed by adding 1 µL RecJf (NEB, M0264L) for ssDNA digestion at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... or IMPa (NEB, P0761). For EndoH and EndoF2-treated samples ...
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bioRxiv - Biochemistry 2024Quote: ... or EndoH (NEB, P0702S) and EndoF2 (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Purified RNA was reverse transcribed with Luna Superscript Mix (NEB). RNA transcripts of viral and host genes were measured by quantitative PCR using primers listed in Table 2 and Power SYBR Green kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) (New England Biolabs). Protein expression was induced with 0.1 mM IPTG and grown overnight at 20°C ...
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bioRxiv - Biochemistry 2024Quote: ... Synthesized cDNA was then diluted 4-fold and 1 μL was used as template for real-time qPCR using Luna Master Mix (NEB). Values were normalized to GAPDH ...
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bioRxiv - Biochemistry 2024Quote: Oligonucleotides were 32P end radiolabeled using T4-polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... gRNA targeting a 20 nt genomic region adjacent to a Cas9 protospacer adjacent motif (PAM) was first annealed and cloned into BsmbI (NEB) digested pLentiCRISPRv2 vector ...
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bioRxiv - Biochemistry 2024Quote: Illumina libraries were prepared using NEBNext Ultra DNA II Library Preparation Kit (New England Biolabs, Ipswich, MA, USA). 500 ng of DNA was fragmented using a Covaris E220 ...
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bioRxiv - Biochemistry 2024Quote: ... 8 units of T7 exonuclease (NEB) were added ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting total RNA was treated with DNase I (NEB, M0303L) to avoid DNA contamination.
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bioRxiv - Bioengineering 2024Quote: ... The DNA was then amplified in the first round of PCR using Q5 DNA Polymerase (NEB), and Illumina barcodes were appended during a second PCR ...
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bioRxiv - Bioengineering 2024Quote: ... Ligations were prepared using T4 RNA Ligase I (New England Biolabs, Cat# M0204) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... were then cloned by HiFi DNA Assembly (New England Biolabs) into the pLew100v5-HYG expression vector (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... Tag variants were generated by ligating synthetic dsDNA oligos (IDT) encoding each respective tag downstream of GFP bewteen BamHI and HindIII sites (NEB). In order to generate the GFP-VapCYALAA fusion ...
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bioRxiv - Biochemistry 2024Quote: ... and T4 DNA ligase (NEB). GFP variants were cloned downstream of a 7xHis-SUMO tag in a modified pET22b vector (EMD Millipore) ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no ...
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bioRxiv - Bioengineering 2024Quote: ... OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (New England Biolabs catalog no. M0488S; New England BioLabs, Ipswich, MA), was used in a 25 µl reaction with 2 µl template DNA for amplification according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2024Quote: ... OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (New England Biolabs catalog no ...
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bioRxiv - Bioengineering 2024Quote: ... and T4 DNA ligase (NEB) to generate cloned sublibraries at high efficiency after electroporation into a cloning strain (ECloni Elite 10G ...
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bioRxiv - Bioengineering 2024Quote: ... treated with RNase A/T1 (New England Biolabs), and purified with ssDNA/RNA Clean & Concentrator kit from Zymo Research.
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no. N0552S; New England BioLabs, Ipswich, MA) and ran at 105 V for 45 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The tag library was cloned into pBAD33 in frame with VapC using BsaI and NcoI endonucleases (NEB) and T4 DNA ligase ...
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bioRxiv - Biochemistry 2024Quote: ... DNA libraries were prepared from cDNA using a two-step PCR process (Q5 Hot Start High-Fidelity DNA Polymerase, New England Biolabs). PCR 1 used Step-1 Forward primer and Step-1 Reverse primer ...
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bioRxiv - Biochemistry 2024Quote: ... The high-fidelity Q5 Polymerase enzyme (NEB) was used for each mutagenesis to amplify the whole plasmid length using the specific SDM primers listed below (Table S5) ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain W [44] (ATCC) gDNA and cloned into pBAD33 [45] using BsaI endonuclease (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The PCR product was digested overnight using Fnu4HI (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... either with or without 1 µL of PNGase F (500 Units, NEB).
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bioRxiv - Biochemistry 2024Quote: ... constructs were generated in the vectors pMal-c2 (NEB), pGEX-2tk (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Linear plasmid-based substrates were generated by digestion with HindIII restriction enzyme (NEB) according to the manufacturer’s instructions followed by heat inactivation ...
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bioRxiv - Biochemistry 2024Quote: 27.4 μg of total RNA was fragmented into ∼150-200 nt fragments at 94 °C for 5 minutes using the magnesium RNA fragmentation buffer (NEB, E6186AVIAL), followed by purification with OCC ...
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bioRxiv - Biochemistry 2024Quote: The library was prepared following our previously reported procedure with slight changes.[21] 3′-End repair and 5′-phosphorylation were conducted with T4 polynucleotide kinase (PNK) (NEB, M0201S). 16 µL RNA was mixed with 2 µL 10× T4 PNK reaction buffer and 1 µL T4 PNK ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL of 20 mg/mL Proteinase K (NEB), and 1 μL of 10 mg/mL RNase A (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: Cy3-labeled enhancer-promoter DNAs used for condensation assay were generated by PCR using Taq polymerase (NEB) with a Cy3-labeled primer (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
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bioRxiv - Biochemistry 2024Quote: ... 400 ng of the purified mRNA was decapped with 20 units of RppH (NEB, #M0356) in 1× Thermopol buffer (TritonX-100 concentration was reduced to 0.01% ...
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bioRxiv - Biochemistry 2024Quote: ... and then 5’ phosphates of the nucleotide sample were removed with 0.5 units of quick-CIP (NEB, #M0525) at 37 ºC for 2 hr with agitation at 800 rpm for 1 min every 10 min.
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... Decapped mRNA was digested to single nucleotides with 50 units Nuclease P1 (NEB, #M0660) in 20 mM NH4Ac at 37 ºC for 12 hr ...
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bioRxiv - Biochemistry 2024Quote: ... MBP-PR18, MBP-TEV-FUS, and MBP-hnRNPA2 (NLS, residues 298‒336) were purified using affinity chromatography (Amylose resin, NEB), and MBP-PR18 was further purified using cation exchange chromatography (HiTrap SP HP ...
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bioRxiv - Biochemistry 2024Quote: ... pETDuet-1 vector was linearized by digestion with PacI and NdeI and gene fragments were inserted into multiple cloning site 2 (MCS2) of the linearized plasmid via Gibson assembly using the New England BioLabs (NEB) Gibson Assembly Master Mix and following the manufacturer’s standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Xrn-1 buffer (New England Biolabs) and Xrn-1 (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... coli RNA Polymerase reaction buffer (New England Biolabs) supplemented with 0.01% Triton X-100 ...
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bioRxiv - Biochemistry 2024Quote: ... and 2,000 nM Rad51 were incubated with 3 μM nt ϕX174 ssDNA (5,386 nt; NEB) in buffer containing 2 mM nucleotide (where indicated) ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain BL21 DE3 pLysIq (NEB no. C3013) through heat shock ...