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Citations for New England Biolabs :
5401 - 5450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Plasmids were linearized using XbaI (New England Biolabs) prior to transformation.
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... PCR reactions to amplify sgRNA and CaCas9 were carried out using Phusion High-Fidelity DNA polymerase (NEB), and Q5 polymerase (NEB ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and Q5 polymerase (NEB) was used for RT amplification ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB5α (New England Biolabs) prior to transformation into the appropriate electrocompetent strains ...
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bioRxiv - Microbiology 2024Quote: ... the samples were pelleted and treated with DNase I (New England Biolabs) at 37°C for 60 minutes and purified on a 20%/50% HistoDenz (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... ΔPYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-ΔPYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Microbiology 2024Quote: ... which was fused to codons 247-1334 of hylA amplified via AOJ_731 and AOJ_732 by digesting both DNA fragments with BspQI (NEB) and ligation using T4 DNA ligase.
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bioRxiv - Microbiology 2024Quote: ... Mutagenesis of RBK21 constructs was carried out using the Q5 Site Directed Mutagenesis kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2024Quote: ... Size-selected cDNA was then PCR amplified for 12 cycles using Q5 High-fidelity polymerase (NEB #M0491S). Amplified libraries were then run on a 6% TBE gel ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Microbiology 2024Quote: ... and treated with shrimp alkaline phosphatase (rSAP)(NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... the construct generated above with the WT rel allele in pIMAY (pRel-swap, Table S2) was used for site directed mutagenesis with the Q5-Site-Directed-Mutagenesis kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and pNJ-26 vector using NEBuilder HiFi DNA Assembly Master Mix (NEB). Tg68 pBAG1:nLuc ...
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bioRxiv - Microbiology 2024Quote: ... and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs) and purified as previously described (55) ...
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bioRxiv - Immunology 2024Quote: ... the wild type and mutant pBlueScript SK(+) HEV plasmids were linearized at their 3′ end with the MluI restriction enzyme (NEB) and transcribed with the mMESSAGE mMACHINE T7 Transcription kit (Ambion #1344) ...
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bioRxiv - Microbiology 2024Quote: Substrate ssRNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, Ipswich, MA, SUA) and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: ... This dsDNA duplex was ligated into the large plasmid digested with KpnI (NEB) and dephosphorylated with rSAP (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The central part of the dsDNA construct was obtained by digestion of the large C-trap plasmid (see above) with NotI (NEB) produced following published protocols (48) ...
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bioRxiv - Microbiology 2024Quote: ... and 5 mM MgCl2) followed by a phosphorylation step of the 5’-terminal ends by the T4 PNK (NEB). This dsDNA duplex was ligated into the large plasmid digested with KpnI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The two gBlocks fragments were assembled into an NdeI-HindIII-cut pET21b backbone using a 2x NEB Gibson assembly master mix (NEB; cat# E2611). To enable such assembly ...
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bioRxiv - Microbiology 2024Quote: ... followed by digestion with the restriction enzyme ApaI or PspOMI (NEB), respectively ...
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bioRxiv - Microbiology 2024Quote: ... and HindIII-HF (NEB, cat# R3104S) and purified by gel extraction ...
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bioRxiv - Microbiology 2024Quote: ... The purified PCR product was assembled with an NdeI-HindIII-cut pIJ10257 backbone using a 2x Gibson master mix (NEB). The resulting plasmids were verified by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2024Quote: ... containing either 400 U/mL of BamHI-HF (NEB; cat# R3136S) or EcoRI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... obtained by digestion with NotI and ApaI (NEB) of the final MT plasmid described above ...
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bioRxiv - Microbiology 2024Quote: ... the tips of DNA-coated probes were immersed in 1x rCutSmart buffer (NEB;cat# B6004S) containing either 400 U/mL of BamHI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... or EcoRI-HF (NEB; cat# R3101S) and incubated for 2.5 hrs at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). This process was conducted at RT for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
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bioRxiv - Microbiology 2024Quote: ... a plasmid derived from pUC19 plasmid previously prepared in our laboratory was digested with KpnI and PshAI (NEB) followed by dephosphorylation ...
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bioRxiv - Microbiology 2024Quote: ... the previous large plasmid containing 1x parS site was digested with NruI (NEB) and dephosphorylated ...
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bioRxiv - Microbiology 2024Quote: ... pET21b was digested with NdeI (NEB, cat# R0111S) and HindIII-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and the eluate was pooled together and used to construct DNA libraries for Illumina deep sequencing using an NEB DNA Library Prep Kit (NEB; cat # E7645S). The prepared DNA libraries were stored at –80°C before being sequenced at the Tufts University Core Facilities (US).
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bioRxiv - Immunology 2024Quote: 7.3 μL volume of PURExpress IVTT master mix (New England Biolabs) consisted of 4 μL solution A ...
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bioRxiv - Immunology 2024Quote: ... were cloned into a pAAV vector using Gibson assembly technique (Gibson et al., 2009) with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, cat. E2621L). AAVs carrying each HDRT were produced as previously described (Hartweger et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... or Luna Superscript master mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The blunt ends were subjected to proximity ligation by 4.17 U/µL T4 DNA ligase (New England Biolabs, M0202) treatment at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by dA tailing with 0.25 U/µL Klenow (3′→5′ exo-) (New England Biolabs, M0212) in the presence of 0.5 mM dATP (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA ends with dA overhangs were ligated to NEBNext Adaptor for Illumina (New England Biolabs) using NEBNext Ultra II Ligation Module (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... the immunoprecipitated DNA was subjected to end repair and adaptor ligation reactions using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645), according to the manufacturer instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (New England Biolabs). The PCR-amplified libraries were purified with 0.9× volume of AMPure XP before sequencing runs with NextSeq 500 ...
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bioRxiv - Molecular Biology 2024Quote: ... and MluCI (New England Biolabs, R0538) restriction enzymes (0.1 U/µL for each enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... HinfI (New England Biolabs, R0155), and MluCI (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The adaptor-ligated DNA on the magnetic beads was amplified by 5-8 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544) and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... using NEBNext Ultra II Ligation Module (New England Biolabs, E7595) and USER Enzyme (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... and with the MboI (New England Biolabs, R0147), HinfI (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.12 U/µL T4 DNA polymerase (New England Biolabs, M0203), and 0.05 U/µL Klenow fragment at 25°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... the protruding ends generated by the restriction digestion were filled in by 0.13 U/uL Klenow fragment (New England Biolabs, M0210) treatment at 37°C for 45 minutes in the presence of 50 µM biotinylated deoxyadenosine triphosphate (biotin-14-dATP ...