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Citations for New England Biolabs :
5601 - 5650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was treated with T4 polynucleotide kinase (New England Biolabs), followed by ligation to a 3′-RNA adaptor using T4 RNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 2 μM of dsDNA was incubated with CpG methyltransferase (M. SssI, New England Biolabs) in the presence of 200 μM of S-adenosylmethionine ...
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bioRxiv - Molecular Biology 2024Quote: ... no SDS (NEB, Cat#B7025S)
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bioRxiv - Molecular Biology 2024Quote: ... and XhoI (NEB). The library amplicons (7 ng ...
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bioRxiv - Molecular Biology 2024Quote: ... was digested overnight with EcoRI and HindIII restriction enzymes (NEB Cat No. R3101, R3104). In order to dephosphorylate the ends ...
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bioRxiv - Molecular Biology 2024Quote: ... each containing 50 ng of DNA (comprising both vector and insert at a 1:3 molar ratio) and high-concentration T4 DNA ligase (NEB Cat No. M0202T). The ligation mix was incubated at 16°C overnight and column-purified with molecular biology-grade water ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL of Disulfide Bond Enhancer 1 and 1 μL of Disulfide Bond Enhancer 2 (New England Biolabs Cat No. E6820S). The reactions were incubated at 37 °C for 8 hours and used immediately or stored at -80 °C ...
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bioRxiv - Molecular Biology 2024Quote: T4 RNA ligase I 10 U/µL (NEB, Cat#M0204S)
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bioRxiv - Molecular Biology 2024Quote: ... complementary synthesized oligonucleotides (Integrated DNA technologies) were annealed and ligated into a lentiGuide- Puro plasmid after BsmBI digestion (New England Biolabs). For the expression of MS2-sgRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... The two fragment products of rpfF and the linearized pK18mobsacB (Schäfer et al. 1994) were then isothermally assembled using the Gibson cloning kit (NEB; Gibson et al. 2009) following the manufacturer’s instructions in a 5:1 insert to backbone ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... Three independent transformations of the Gibson Assembly reactions were carried out using NEB 10-beta electrocompetent E.coli (NEB #C3020K) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: The lentiviral gRNA expression vector pKLV3-U6gRNA5(BbsI)-PGKpuroBFP-W-L1 (Ong et al. 2017) was digested with BbsI-HF (NEB #R3539) and then gel purified ...
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bioRxiv - Molecular Biology 2024Quote: RNase H 5 U/µL (NEB, Cat#M0297S)
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bioRxiv - Neuroscience 2024Quote: Genomic DNA from SH-SY5Y cells and iPSCs was extracted with the Monarch Genomic DNA Purification Kit (NEB). PCR was performed with Q5 Hot Start High-Fidelity Master Mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... or HiScribe T7 ARCA mRNA Kit (New England Biolabs), evaluated spectrophotometrically and by gel electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760L). Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (5067-5582 and 5067-5583) ...
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bioRxiv - Neuroscience 2024Quote: ... and hybridized with NEBNext rRNA Depletion Kit v2 (NEB E7400) to substantially diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep for Illumina (New England Biolabs, E7645S and E7645L) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... where biotin-labeled primer (Bio-R1) was supplemented together with 2x NEB Next Ultra II Q5 Master Mix (M0544L, NEB). LAM products were purified with Streptavidin C1 dynabeads (65001 ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Neuroscience 2024Quote: ... with AgeI-HF and EcoRI-HF and annealed shRNA sequences were ligated with Quick Ligase Buffer (NEB). Primers for oligos against the listed sequences followed the format:
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of total RNA was used with Luna Universal One-Step RT-qPCR (NEB, Ipswich, MA) to detect StayGold mRNA expression ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was eluted in 50 µl of nuclease free H2O (New England BioLabs). Subsequent cDNA generation was set up using the LunaScript® RT SuperMix Kit and its corresponding manual ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA constructs used for targeting mutations were made using the Gibson method (NEB, #E2621L) with details provided in the supplemental Materials and Methods.
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bioRxiv - Molecular Biology 2024Quote: ... a two-step process was used in which cDNA was first synthesized with oligo dT priming using the ProtoScript II First-Strand cDNA Synthesis kit (New England BioLabs, Ipswich, MA). To denature the template RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... after removing Cas9 with Restriction Endonucleases: NheI (NEB R0131L) and BamHI-HF (NEB R3136L) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 µl Q5 High-Fidelity DNA Polymerase (NEB, cat. no. M0491), 24.68 µl dH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... calf intestinal phosphatase (NEB), and antarctic phosphatase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was used with the EpiMark Bisulfite Conversion Kit (NEB) using the manufacturer instructions.
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bioRxiv - Neuroscience 2024Quote: ... or Q5 Site-Directed Mutagenesis Kit (New England Biolabs). To improve PCR yield with the GCrich hα1B template ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with Monarch PCR cleanup kit (NEB). Purified amplicons were sequenced by Plasmidsaurus ...
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bioRxiv - Neuroscience 2024Quote: ... The GfaABC1D-tdTom vector was linearized at NheI and NotI (New England Biolabs R3131S, R1089S) restriction sites for three hours at 37C and the linearized vector was purified from an agarose gel and cleaned using Nucleospin columns (Macherey-Nagel 740609.50) ...
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bioRxiv - Molecular Biology 2024Quote: ... before undergoing ligation using T4 DNA ligase (NEB). Constructs were sequenced by Sanger sequencing to check the IDRs had been correctly removed from the construct.
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bioRxiv - Molecular Biology 2024Quote: ... and CBPΔCFID-GFP for double mutants) were digested using NruI and PmlI (NEB). The digested constructs were dephosphorylated using Quick Calf Intestinal Phosphatase (CIP ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using the Monarch RNA Cleanup Kit (T2040L, New England Biolabs). Quality and size of the produced RNAs were assessed using an Agilent Fragment Analyzer.
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bioRxiv - Molecular Biology 2024Quote: ... the ends of the PCR fragments were then phosphorylated using T4 polynucleotide kinase (PNK, NEB) before undergoing ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragments were annealed using T4 DNA ligase (NEB) before sequencing to check for correct insertion and subsequently by whole plasmid sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... and the Yeast Inorganic Pyrophosphatase (Cat. No. M2403S, New England BioLabs) following the CleanCap protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... pHR-mCh-Cry2WT and the synthesised NLS were ligated using T4 DNA ligase (NEB) and the construct was checked by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... CBPwt-GFP and synthesised sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested CBPwt-GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a hygromycin resistance cassette using BamHI-HF and NotI-HF (NEB) restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... and Taq DNA polymerase (all NEB). After purification with the High Pure PCR Cleanup Micro Kit (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... consist of EcoRV-NdeI (NEB) pAG3 fragment as a plasmid backbone ...
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bioRxiv - Molecular Biology 2024Quote: ... the target site was amplified by Taq DNA polymerase:DeepVent polymerase 200:1 U (both NEB) with CRISPR efficiency primers and sequenced ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μM forward and reverse oligo pairs were combined with T4 polynucleotide kinase (PNK) (NEB) and 10X T4 ligation buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... These three DNA fragments were further assembled using the NEBuilder HiFi DNA Assembly cloning kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2024Quote: ... The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB) restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... before assembly using T4 DNA ligase (NEB). Correct insertion was checked by Sanger sequencing.