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Citations for New England Biolabs :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB, S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100 T zymolyase (US Biological ...
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bioRxiv - Molecular Biology 2024Quote: ... The oligonucleotide-based DNA substrate used for the helicase and nuclease assays was generated by labeling the BIO100C oligonucleotide (see Table S1 for oligonucleotides sequence) at the 3’ end using terminal transferase (New England Biolabs) and [α-32P]dCTP (Hartmann Analytic ...
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bioRxiv - Molecular Biology 2024Quote: ... 1µg fragment DNA was end repaired using NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546). PCR adapters from Oxford Nanopore Technologies (ONT #SQK-PSK004 ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103). DNA was sequenced on Illumina HiSeq 2500 platform.
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bioRxiv - Molecular Biology 2024Quote: ... 3.2 U/ml Nt.CviPII (NEB #R0626S) and 64 U/ml E ...
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bioRxiv - Neuroscience 2024Quote: ... long amplification of the CHRFAM7A intron (exon A to exon 5) was carried out with the LongAmp® Taq DNA Polymerase Kit (ref. E5200S, New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 volumes 10 mM MnCl2 and 10 volumes of 10x PMP buffer (NEB). The phosphatase mix for comparing untreated with treated samples contained 1:20 λ-phosphatase in 1 mM MnCl2 and 1x PMP-buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 1 volume of λ-Phosphatase (NEB) with 1 volume of water ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with 4 units of Dam enzyme (NEB, M0222L). We use Damonly control samples to normalize for DNA accessibility and amplification biases as described (Vogel et al ...
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bioRxiv - Molecular Biology 2024Quote: A 2-fold excess of either m7G(5’)ppp(5’)G RNA Cap Structure Analog (New England Biolabs) or chemically synthesized cap4 hexa-nucleotide (see cap4 synthesis below ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 hour at 25°C with 20% PEG8000 (New England Biolabs). After ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... designed for small RNA library preparations (New England Biolabs, cat# N3032S). Primers ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was 5’-labelled with 20 U of T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB # M0236S) and 50 pmol of (gamma-32P)ATP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were subsequently ligated to deacylated and demethylated RNA templates using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 hour at 25°C with 20% PEG8000 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids encoding for NanoBiT TMD fusion proteins were cloned using the NEBuilder system (New England Biolabs, Ipswich, MA, USA). Promega NanoBiT plasmid backbone (pBiT_1.1-C[TK/LgBiT] ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Q5 High-Fidelity DNA Polymerase (M0491S, NEB) was used with 200 ng of template DNA in a total reaction volume of 20 µL.
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bioRxiv - Molecular Biology 2024Quote: ... was digested with Nucleoside Digestion Mix (NEB) at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 350 ng of purified DNA was restricted (according to manufacturer’s instructions) with DpnI (NEB) or DpnII (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 25 μg was then diluted to 100 μL with CP-1 buffer with or without 2 μg/mL proteinase K (New England Biolabs P8107S) and the indicated concentration of digitonin or 2% Triton ...
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bioRxiv - Molecular Biology 2024Quote: ... using NEBuilder HiFi DNA assembly kit (NEB). pBAD-His6-Ocr was obtained by cloning 0.3 gene from T7 phage into pBAD-HisB43 vector using Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pHERD30t with and without overlapping AluI/BREX site was purified from BREX+ (E. coli HS) or BREX- cells (E. coli BW25113) using Monarch Plasmid Purification Kit (NEB). 100 ng of plasmid was restricted with AluI (ThermoScientific ...
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bioRxiv - Molecular Biology 2024Quote: ... pBAD-His6-Ocr was obtained by cloning 0.3 gene from T7 phage into pBAD-HisB43 vector using Gibson Assembly Master Mix (NEB). Introduction of the overlapping BREX/AluI site to the pHERD30t vector was also achieved via Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Introduction of the overlapping BREX/AluI site to the pHERD30t vector was also achieved via Q5 site-directed mutagenesis kit (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... or DpnII (NEB) to monitor Dam site methylation status or with AluI (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Five micrograms of genomic DNA derived from G418R colonies were digested to completion with SspI or ApoI (NEB) in a total reaction volume of 50 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... Monarch RNA Cleanup kit (T2030L, NEB), Murine RNase inhibitor (M0314 ...
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bioRxiv - Molecular Biology 2024Quote: ... One microliter of the LEAP reaction then was amplified in a 25 μL PCR reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with primers specific for sequences within the SV40 promoter of the neoTet and mneoI reporter cassettes (NeoSV40_fwd ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’ adapters with 4 terminal randomized nucleotides at the 3’ end was added using T4 RNA ligase (New England Biolabs, cat# M0204S). Ligation was carried out for 1 hour at 25°C with 20% PEG8000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μL of the diluted cDNA reaction was used in PCR reactions (25 μL) using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with NeoSV40_fwd and 3′LEAPOuter primers ...
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bioRxiv - Molecular Biology 2024Quote: ... The restricted DNAs were ligated using 8 μL (3200U) of T4 DNA ligase (NEB) in a volume of 600 μL at 16°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μl of dTTP (1 mM, NEB Catalog # N443S), 1 μL of Tdt buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Other plasmids were cloned by Gibson Assembly using NEBuilder HiFi (New England Biolabs). Cloning strains used were NEB Stable (lentiviral ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Microbiology 2024Quote: ... amplified plasmid and insert PCR products were assembled using NEBuilder HiFi DNA Master Mix (New England Biolabs) following manufacturer’s protocols and transformed into Escherchia coli strain S-17 by electroporation for maintenance and replication ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs). The samples were sequenced using a NextSeq 500/550 Mid Output V2.5 kit (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... Library quantification was done using NEB Next® Library Quant Kit for Illumina® (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were built using an NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645S/L) with immunoprecipitated or input DNA ...
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bioRxiv - Microbiology 2024Quote: ... ligation-based library preparation was performed using a NEBNext Ultra II DNA Kit (NEB). The library was then subjected to Illumina NovaSeq paired-end sequencing (150-bp).
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bioRxiv - Microbiology 2024Quote: ... TdT reaction buffer (1.5 µl), CoCl2 (5 µl, 0.25 mM) (NEB #B0252), and filtered H2O (4.5 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... phosphorylated using the T4 polynucleotide kinase enzyme (NEB), and annealed by incubating at 95 °C for 5 min followed by a temperature ramp of −5 °C every minute ...
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bioRxiv - Molecular Biology 2024Quote: ... purified PCR products were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB). Assembled gRNA/Cas9 and donor plasmids were transformed into DH5α bacteria and final purified plasmids were checked by sanger sequencing (Genewiz).
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bioRxiv - Molecular Biology 2024Quote: ... A 1:200 dilution of the double-stranded gRNA was ligated into BsmBI-digested plasmids using T4 ligase (NEB). One µL of the ligated vector was then transformed into chemically competent XL Gold E ...
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bioRxiv - Microbiology 2024Quote: ... 75 μL amylose resin (#E8021S, New England Biolabs) was added to a Bio-Spin disposable chromatography column (#7326008 ...
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Microbiology 2024Quote: ... These flanking pieces were cloned into BamHI-digested pLBG1347 using NEBuilder (New England BioLabs) and transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... phosphorylated with T4 PNK (NEB) and cloned into the pU6-(BbsI)_CBh-Cas9-T2A-mCherry plasmid96 (a gift from Ralf Kuehn ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into the vector with T4 ligase (NEB). The multiple cloning sites allow adding new or replacing all features ...
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bioRxiv - Molecular Biology 2024Quote: ... berghei ANKA cl15cy1 genomic DNA and cloned into the pYCs and pYCm plasmids following digestion with KasI and StuI (NEB), using the NEBuilder HiFi DNA reaction master mix ...
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bioRxiv - Microbiology 2024Quote: ... The DNA fragments was mixed with 10 μL of streptavbidin coated meganetic beads (NEB Catalog # S1421S) and 500 μL of binding buffer (5 mM Tris-HCl ...