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Citations for New England Biolabs :
5351 - 5400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 4.5 μl of 10mM dATP (NEB, N0440S), 4.5 μl of 10mM dGTP (NEB ...
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bioRxiv - Genomics 2024Quote: ... 15 μl of 400 U/μl T4 DNA ligase (NEB, M0202L), followed by 4h incubation at RT with gentle rocking ...
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bioRxiv - Genomics 2024Quote: ... 24μl of 5U/μl DNA polymerase I Large (Klenow; NEB, M0210L) and the samples were incubated for 1h at 37°C with rotation ...
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bioRxiv - Genomics 2024Quote: ... ligated with a hairpin adaptor (NEB), treated with USER enzyme (NEB ...
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bioRxiv - Genomics 2024Quote: ... Ligation was performed by addition to the sample of Ligation master mix (2007 μl of water, 300 μl of 10% Triton X-100, 18 μl of 10 mg/ml BSA (NEB, B9000S), 360 μl 10x T4 DNA ligase buffer (NEB ...
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bioRxiv - Genomics 2024Quote: ... 4.5 μl of 10mM dGTP (NEB, N0442S), 45 μl of 1mM biotin-16-dCTP (Jena Bioscience NU-809-BIO16-S) ...
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bioRxiv - Genomics 2024Quote: ... 4.5 μl of 10mM dTTP (NEB, N0443S), 4.5 μl of 10mM dATP (NEB ...
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bioRxiv - Genomics 2024Quote: ... and Exonuclease I (NEB). In vitro cleavage reactions were performed with 125 ng of exonuclease-treated circularized DNA ...
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bioRxiv - Genomics 2024Quote: ... and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB), using Kapa HiFi Polymerase (KAPA Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
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bioRxiv - Genomics 2024Quote: ... PCR amplifications were performed using phusion hot start flex 2X master mix (NEB, M0536S) and 20-100ng genomic DNA with the following conditions for HBG-sgRNA target (denaturation - 98°C for 3:00 ...
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bioRxiv - Genomics 2024Quote: ... Gap-repaired tagmented DNA was treated with USER enzyme (NEB) and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB) and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen) ...
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bioRxiv - Genomics 2024Quote: ... and Taq DNA ligase (NEB). Gap-repaired tagmented DNA was treated with USER enzyme (NEB ...
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bioRxiv - Genomics 2024Quote: ... and T4 polynucleotide kinase (NEB). Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... Lambda exonuclease (NEB) and Exonuclease I (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The work started with amplification of apramycin resistance cassette (Sequence S1) by Q5 polymerase (New England Biolabs) using the primer pair bamB-strep-F and bamB-strep-R (Table S4) ...
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bioRxiv - Immunology 2024Quote: ... and amplified using Next High-Fidelity 2x PCR Master Mix (New England Biolabs #M0541) with Nextera DNA CD Indexes ...
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bioRxiv - Molecular Biology 2024Quote: ... This incubation was repeated with a 1:200 pA-Dam solution (equivalent to nearly 60 Dam units, determined by calibration against Dam enzyme from NEB, #M0222L), followed by two wash steps ...
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bioRxiv - Molecular Biology 2024Quote: ... and 25 µL 2X NEBNext high-fidelity PCR master mix (NEB, M0541). To reduce GC/size bias and over amplification of libraries ...
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bioRxiv - Molecular Biology 2024Quote: ... Murine RNase inhibitor (M0314, NEB), Nitrocellulose membrane (1620115 ...
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bioRxiv - Molecular Biology 2024Quote: ... Streptavidin Magnetic Beads (S1420S, NEB), Streptavidin-IR800 (926-32230 ...
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bioRxiv - Molecular Biology 2024Quote: Visible LEAP bands were excised from agarose gels and cDNAs were purified using the Monarch DNA Gel Extraction Kit (NEB). Purified DNAs from excised gel slices were cloned into a pCR4Blunt-TOPO vector using a Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain NEB10-beta (New England Biolabs #C3019H) grown in LB-medium (Sigma-Aldrich #L3022 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were constructed using either T4 ligation (New England Biolabs #M0202L) or HiFi DNA Assembly methods (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... T4 polynucleotide kinase reaction buffer (B0201S, NEB), T4 RNA ligase (M0204S ...
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bioRxiv - Molecular Biology 2024Quote: ... T4 RNA ligase 2 (M0239S, NEB), Leupeptin (PI78435 ...
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bioRxiv - Molecular Biology 2024Quote: ... or HiFi DNA Assembly methods (New England Biolabs, #E2621S). All plasmids used in this study are listed in Table S4.
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bioRxiv - Molecular Biology 2024Quote: ... T4 polynucleotide kinase (M0201S, NEB), T4 polynucleotide kinase reaction buffer (B0201S ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5 Site-Directed Mutagenesis Kit (E0554S, NEB), Recombinant protein G sepharose beads (101242 ...
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bioRxiv - Molecular Biology 2024Quote: ... T4 RNA ligase (M0204S, NEB), T4 RNA ligase 2 (M0239S ...
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bioRxiv - Molecular Biology 2024Quote: gBlock DNA was digested out of the TOPO vectors using BstXI (New England Biolabs), sites which are present in the vector on either side of the insert and are absent from gBlocks ...
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bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
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bioRxiv - Molecular Biology 2024Quote: ... 27.5 µl and 12.5 µl of NEBNext Sample Purification Beads (New England Biolabs) were used in the first and second steps ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The 50 µl PCR product with 10 µl of 6X Purple Loading Dye (New England Biolabs) was electrophoresed through a 50 ml gel – 1% SeaKem Agarose (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: DICER RNA substrates have been obtained using PCR synthetized linear DNA bearing T7 promoter and HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040S) following manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Length of DNA Fragments were determined by agarose gel electrophoresis and then purified with Monarch DNA Gel Extraction Kit (NEB # T1020L). DNA concentration has been assessed by Nanodrop.
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bioRxiv - Molecular Biology 2024Quote: T7 in vitro transcribed (IVT) RNA (DS2_AS) was dephosphorylated with Quick CIP (NEB # M0525S) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... ssDNA for DNA:RNA heteroduplex (400nt) has been prepared using phosphorylated primer and Lambda Exonuclease (NEB # M0262S). Length of DNA Fragments were determined by agarose gel electrophoresis and then purified with Monarch DNA Gel Extraction Kit (NEB # T1020L) ...
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bioRxiv - Immunology 2024Quote: ... library fragments were amplified using 1x NEB Next PCR master mix (New England BioLabs) and 1.25 μM of custom Nextera PCR primers forward and reverse ...
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bioRxiv - Microbiology 2024Quote: ... OneTaq HS Quick-Load (New England Biolabs) was used to amplify the genomic barcodes by PCR for 25 cycles ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the agarose gel fragments using a gel extraction kit (New England Biolabs, T1020L) following manufacturer instructions with modification ...
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bioRxiv - Microbiology 2024Quote: ... and dephosphorylated with rSAP (NEB). This resulted in a large plasmid with a single parS site ...
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bioRxiv - Microbiology 2024Quote: ... Labeled handles were ligated to the central part overnight using T4 DNA Ligase (NEB). The sample was then ready for use in MT experiments without further purification ...
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bioRxiv - Microbiology 2024Quote: ... the dsDNA duplex once annealed was digested with SfoI and HpaI (NEB). The digested dsDNA duplex was ligated into the linearized large plasmid ...
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bioRxiv - Microbiology 2024Quote: ... the dsDNA duplex with 2x parS sites digested with XhoI and SalI (NEB) was ligated into the plasmid with 3x parS site digested with XhoI and dephosphorylated ...
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bioRxiv - Immunology 2024Quote: ... We added proteinase K (8 U/ml, NEB #P8107S) immediately before use and incubated the gel at 37 °C for 1 hour in a 35 mm dish ...
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bioRxiv - Microbiology 2024Quote: ... Samples get 10-fold diluted and procedure was performed using Luna® Universal qPCR Master Mix (New England Biolabs, USA) and rpoD housekeeping gene as reference ...
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bioRxiv - Microbiology 2024Quote: The intermediate strain nAB094 was generated by transforming nAB019 with a plasmid assembled using Gibson Assembly (New England Biolabs) containing 4 fragments ...