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Citations for New England Biolabs :
5651 - 5700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 µl m7G(5′)ppp(5′)G RNA Cap Structure Analog (10 μmol) (New England BioLabs, Ipswich, MA), 2 µg linearized DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... using NEBuilder HiFi DNA Assembly reaction protocol (NEB). CBP was amplified using primers ...
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bioRxiv - Molecular Biology 2024Quote: ... before the inserts and vectors were assembled using T4 DNA ligase (NEB). The assembled constructs were checked by Sanger sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... DNA was fragmented by DNA fragmentase (NEB) to obtain fragments 100-2000 bp long with the main density between 500-1000 bp on an agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... we ligated adaptors with Quick Ligase (NEB). Apart from the standard adaptor oligos Ion-Link-A and Ion-Link-Ba ...
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bioRxiv - Molecular Biology 2024Quote: ... gamma-mCherry or NaeI-EcoRV (NEB) pAG3-mCherry fragment containing proviral sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... in NcoI and SalI (NEB) restriction sites by In-Fusion® HD Cloning Kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... the MfeI-EcoRV (NEB) gamma-mCherry or NaeI-EcoRV (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl T7 RNA Polymerase (New England BioLabs, Ipswich, MA), 5 µl ATP (10 μmol) ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro RNA transcription was performed in 50 µl reactions: 5 µl RNAPol Reaction Buffer (New England BioLabs, Ipswich, MA), 2 µl T7 RNA Polymerase (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µl GTP (10 μmol) (New England BioLabs, Ipswich, MA), 7.5 µl m7G(5′)ppp(5′)G RNA Cap Structure Analog (10 μmol ...
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bioRxiv - Molecular Biology 2024Quote: ... apyrase (1 unit NEB) was added for an additional 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of purified RNA was immobilized on streptavidin magnetic beads (NEB) for 8 h at 4°C under orbital rotation in binding buffer (24 mM KCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA samples were then used for library preparation using NEBNext UltraII DNA Library prep kit (NEB, E7370L) and were sequenced at the iGE3 platform of the University of Geneva.
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were subject to end-repair and dA-tailing with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), followed by ligation with 50 pmol of adaptor 1 (Ad1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation products were purified by 1.1×DNA FragSelect XP Magnetic Beads and amplified by 12-15 cycles of PCR with NEBNext Ultra II Q5 Master Mix and NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... This dimer was introduced into pBECAb-apr using the NEBridge Golden Gate Assembly Kit (New England Biolabs, Ipswich, MA, USA) to generate pBECAb-apr-kmtA ...
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bioRxiv - Molecular Biology 2024Quote: ... Following restriction digestion with NotI and SbfI (NEB), pHR-mCh-Cry2WT and the synthesised NLS were ligated using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: T4 PNK 10 U/µL (NEB, Cat#M02021S)
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bioRxiv - Molecular Biology 2024Quote: ... The plasmids were transcribed in-vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs Cat No. E2050S) to produce RNA ...
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bioRxiv - Molecular Biology 2024Quote: RNA of translated using the PURExpress ΔRibosome Kit (New England Biolabs Cat No. E3313S). The translation reactions contained 0.4 μM mRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (New England Biolabs) in PDM media57 or Lysogeny Broth (LB) ...
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bioRxiv - Molecular Biology 2024Quote: ... A second adapter was then ligated to the 3’OH of the cDNAs (with NEB HC RNA Ligase in NEB ligation buffer plus 5% DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... All cloning was performed using Gibson assembly cloning (NEB). All cDNA constructs were confirmed by sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs) and validated by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested constructs were dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB), before the inserts and vectors were assembled using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Restriction enzymes including BsmBI-v2 (NEB, R0739), NheI-HF (NEB ...
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bioRxiv - Molecular Biology 2024Quote: 1µg of RNA was reverse transcribed into cDNA using random hexamers and Protoscript II Reverse Transcriptase (NEB). cDNA was diluted to 50µl ...
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bioRxiv - Molecular Biology 2024Quote: ... Quick change was performed using Q5® Site-Directed Mutagenesis Kit (NEB: E0554S) to mutate the lysines at position 26-27 to glutamines or alanines ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were run on 1.5% agarose gel alongside 50-bp DNA ladders (NEB #N0556S). Image Lab software (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was poly(A)-selected using the NEBNext® High Input Poly(A) mRNA Isolation Module (NEB, E3370S). The final elution step of this protocol was performed using RNase-free ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was carried out on the purified DNA using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced at 100 million reads per sample on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared by the EPFL Gene Expression Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760S), and sequenced on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: All consumable enzymes for restriction ligation and Gibson assembly were procured from NEB (New England Biolabs, Ipswich, MA) including BsaI-HF-v2 (NEB #R3733S) ...
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bioRxiv - Molecular Biology 2024Quote: ... MNase (NEB) was added to 2 x 106 nuclei in 50 µL reaction volume with 250– 2000 units of enzyme that was incubated for 5 minutes at 37 °C before 1 mM of CaCl2 was added and incubation continued for another 5 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: Replication deficient adenovirus plasmids were linearized using PacI enzyme (NEB, Cat# R0547) as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Inserts and fragments generated by PCR were synthesized using Q5 High Fidelity DNA Polymerase (NEB, M0491). All primers were synthesized by Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated DNA was washed once with 70% ethanol and suspended in 30μl 0.1 X TE (1mM Tris-HCl and 0.1mM EDTA) for library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Ligation of Unique Dual Index UMI Adaptors (NEB). Barcoding PCR was consistently carried out with NEBNext High-Fidelity 2X PCR Mix and subsequent size selection using agarose gel or beads (Ampure XP) ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 2X B&W buffer supplemented with 0.4 U/µL RNase Inhibitor (Murine) (NEB, M0314S) to a bead concentration of 5 ug/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Ultra II Q5 Master Mix (NEB, M0544S) in a 50 µL final reaction volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Final vectors were assembled using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create pGL3 plasmids containing VEGFAe ...
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bioRxiv - Molecular Biology 2024Quote: Omni-ATAC or CUT&Tag libraries assembled into the pLenti-STARR vector using HiFi assembly (NEB, E5520S), as previously described [Arnold et al ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed using NEBNext Polymerase (NEB) using the primer sets listed in Supplementary Table 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using ProtoScript II Reverse Transcriptase (New England Biolabs #M0368L) and poly(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... and ATP and m7GpppAm cap analog (New England Biolabs) at a 1:5 ratio.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM phosphoenolpyruvate and 0.15 mg/ml bovine serum albumin (New England Biolabs), in a final volume of 15 µl ...