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Citations for New England Biolabs :
5501 - 5550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: dam enzyme (NEB, Cat# M0222S) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... IVT was performed following the manufacturer’s protocol for the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Polyadenylated RNA was added to the IVT RNA control at a ratio of 1:1000.
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was used as the template for IVT with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragmentation was performed using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs), an enzymatic fragmentation assay with an average fragment size of 380 bp ...
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bioRxiv - Genomics 2024Quote: ... The single-strandedness of the DNA oligos on the beads was restored by incubating the beads with a lambda exonuclease mix (NEB M0262L) for 30 minutes at 37° C ...
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bioRxiv - Immunology 2024Quote: ... 2 × RNA loading dye (NEB, #B0363S) were added to the system to stop the reaction and the RNA was separated by agarose gel electrophoresis.
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bioRxiv - Plant Biology 2024Quote: ... with primers listed in Supplementary Table S6and cut by BamHI and SalI (New England Biolabs). The DNA fragments were cloned into a pTV00 vector using T4 DNA ligase (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using 0.4 µg of RNA and the NEBNext ® UltraTM RNA Library Prep Kity for Illumina (NEB, USA). mRNA was purified via poly-T magnetic beads ...
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bioRxiv - Plant Biology 2024Quote: ... the cDNA was pre-amplified with 6 cycles using Phusion HF Mastermix (New England Biolabs) and size-selected using the ProNex Size-Selective Purification System (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... 260 to 300bp mRNA (CDS or UTR) sequences of enzymes were amplified by PCR using Q5® High-Fidelity DNA Polymerase (New England Biolabs) with primers listed in Supplementary Table S6and cut by BamHI and SalI (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... we used a 1:1 mix of Low Range ssRNA Ladder (New England Biolabs™, catalog no. N0364S) and 14-30 nt ssRNA Ladder Marker (Takara™ ...
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bioRxiv - Plant Biology 2024Quote: ... Purified PCR fragments were assembled using Gibson Assembly® Master Mix (New England Biolabs, MA, USA) following the manufacturer’s procedures.
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bioRxiv - Plant Biology 2024Quote: ... NLS-eGFP-FLAG-SRDX and NLS-eGFP-FLAG were assembled through NEBuilder (New England Biolabs) and cloned by BP reaction ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated from leaves 4 and 5 using Trizol and cDNA was synthesized using MuMLV reverse transcriptase (New England Biolabs, Inc.) primed with random hexamers ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid standard was linearised using SpeI restriction enzyme (New England Biolabs) and serially diluted ...
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bioRxiv - Synthetic Biology 2024Quote: ... up to 2 μg of plasmid DNA was incubated for 4 h at 37°C with 2 μl of CpG Methyltransferase from M.SssI (NEB) in the Methyltransferase Reaction Buffer supplemented with 2 μl of diluted SAM (6.4 mM) ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded libraries were made with NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs). Libraries were pooled and sequenced (single-end 75-bp reads ...
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bioRxiv - Immunology 2024Quote: ... MO were stored at 4°C in water at 1 mM and diluted for injections with 0.5X CutSmart Buffer (NEB) and 0.1% phenol red ...
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bioRxiv - Plant Biology 2024Quote: ... 10 mM Ribonucleoside Vanadyl Complex (NEB) and 100 U/ml Ribolock (Thermo Fisher Scientific)) ...
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bioRxiv - Plant Biology 2024Quote: ... we used NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7760L) protocol ...
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bioRxiv - Plant Biology 2024Quote: ... then transformed into DH5α competent Escherichia coli (C2987H, New England Biolabs, UK) following standard manufacturer protocol ...
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bioRxiv - Plant Biology 2024Quote: ... and assembled into the backbone (Kpn1 and BamH1 digested) of pCHYG by Gibson Assembly (NEB, UK). The resulting plasmids were transformed into Agrobacterium strain AgL1 and fungal transformation of isolate IPO323 was performed as per standard protocols (Motteram et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Flanking sequences and the hygromycin resistance gene were amplified from either genomic DNA or from plasmid pCHYG and using Phusion polymerase (NEB, UK). Fragments were gel purified using QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Plant Biology 2024Quote: ... Using the Gibson Master Mix (New England Biolabs, UK) the paired split marker fragments were ligated into the pGEM® -T Easy Vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... Frozen samples were ground and RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, UK). Equal amounts of RNA was used to synthesise cDNA with Revertaid cDNA synthesis kit (ThermoScientific ...
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bioRxiv - Biophysics 2024Quote: ... and subsequent restriction digest of the plasmid DNA was carried out using the BamHI restriction enzyme (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... were deglycosylated by incubation with PNGase F (New England BioLabs) at 37 °C for one hour ...
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bioRxiv - Biochemistry 2024Quote: ... Twelve cycles of amplification were carried out with Q5 High Fidelity DNA Polymerase (NEB) using an annealing temperature of 68 °C and a 1 min extension at 65 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were then assembled via Gibson assembly (New England Biolabs, Cat # E2611L), followed by transformation of E ...
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bioRxiv - Neuroscience 2024Quote: ... The promoter was cloned using the Gibson Cloning Assembly Kit (New England BioLabs) following standard procedures ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR products of the ORF of CrHCF244 and linear vectors were assembled by Gibson assembly (Gibson et al., 2009) using Gibson Assembly® Master Mix (New England Biolabs). For the vector construct to transform the Arabidopsis HCF244 gene into Chlamydomonas ...
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bioRxiv - Neuroscience 2024Quote: ... The homology-directed repair (HDR) plasmid for C-terminal SAX-2::GFP(ju1831) was made using three-fragment Gibson Assembly (New England Biolabs) in which two sax-2 homology arms containing 498 bp upstream of the sax-2 TAA stop codon and 540 bp downstream of the sax-2 TAA stop codon plus the stop codon were amplified from N2 genomic DNA and fused such that they flanked GFP (pDD282) ...
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bioRxiv - Neuroscience 2024Quote: ... and the variable regions of the AAV library amplified using serotype-specific primers and the NEB Q5 2x Master Mix (New England Biolabs, Ipswich, MA). Amplicons were incorporated into recipient plasmids by Gibson assembly and transformed into Endura electrocompetent cells (LGC ...
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bioRxiv - Neuroscience 2024Quote: ... The four separate fragments were amplified and inserted into a vector containing the Tol2 sites35 using the Gibson Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... The lysate was then treated with 20k units of a2-3,6,8 neuraminidase (New England BioLabs) overnight at 37°C with end-over-end rotation ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Plant Biology 2024Quote: ... a NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted using the New England Biolabs Monarch Total RNA Miniprep Kit (NEB #T2010, Ipswich, MA, USA). Acetone was removed from the tubes under the dissecting scope ...
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bioRxiv - Plant Biology 2024Quote: ... The generated level 0 tRNA-sgRNA modules were then combined via another cut-ligation reaction using BsaI-HF®v2 (New England Biolabs) into two L1 transcription units (C441001 and C441002 ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were generated using the NEBNext Multiplex Small RNA Library Prep Set (#E7300S, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The Phusion High Fidelity DNA polymerase (New England Biolabs) was used for all the PCR reactions ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was further processed using the Monarch RNA cleanup Kit (New England Biolabs, Ipswich, MA). Protocol steps were executed according to manufacturer’s instructions with slight modifications and the RNA was finally eluted in a total volume of 40ul of RNase/DNase free water ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using the NEBNext® Enzymatic Methyl-seq Kit (NEB, #M7634) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 400 U Lambda Protein Phosphatase (New England Biolabs, Frankfurt, Germany) in NEBuffer ...
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bioRxiv - Plant Biology 2024Quote: ... and purified using Amylose Resin (New England Biolabs, Ipswich, MA). 0.5 - 1 μg purified GST-PIN3 cytoplasmic loop (CL ...
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bioRxiv - Neuroscience 2024Quote: ... BL21 Escherichia coli cells (New England Biolabs, Ipswich, MA) expressing recombinant CRMP5-GST were resuspended in 50 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... the guides and donor were combined into the pFW lentiviral backbone18 by NEB HIFI Assembly ...
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bioRxiv - Neuroscience 2024Quote: ... FLEx-mTagBFP2 was generated by NEB HIFI Assembly ...
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bioRxiv - Neuroscience 2024Quote: ... and T4 DNA ligase (New England Biolabs, USA). The plasmids were sequenced and SH-SY5Y cells were cultured and transfected as described above ...